A device for investigation of natural cell mobility and deformability
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
Strategy program (Qualitas)
Akademie Věd České Republiky - International
17-01995s
Grantová Agentura České Republiky - International
PubMed
32358820
DOI
10.1002/elps.201900357
Knihovny.cz E-zdroje
- Klíčová slova
- Cell deformability, Cell mobility, Confocal microscopy, Metastasis, Microfluidics,
- MeSH
- buněčné kultury přístrojové vybavení MeSH
- design vybavení MeSH
- dimethylpolysiloxany chemie MeSH
- HeLa buňky MeSH
- konfokální mikroskopie MeSH
- lidé MeSH
- mikrofluidní analytické techniky přístrojové vybavení MeSH
- pohyb buněk fyziologie MeSH
- tvar buňky fyziologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- baysilon MeSH Prohlížeč
- dimethylpolysiloxany MeSH
A microfluidic device made of polydimethylsiloxane was developed for continuous evaluation of natural migration mobility of many eukaryotic cells in relaxed and deformed state. The device was fabricated by standard photolithography and soft lithography techniques using the SU-8 3010 negative photoresist on a glass wafer as the master mold. The simple flow-free device exploits the chemotactic movement of cells through a set of mechanical barriers in the direction of concentration gradients of attractants. The barriers are formed by arrays of circular cross-section pillars with decreasing spacing 7, 5, and 3 μm. To pass through the obstacles, the cells are deformed and change their cytoskeletal architecture. The instantaneous migration velocities of cells are monitored in a time-lapse setup of the scanning confocal microscope. Thus, the cellular deformability and migratory activity can easily be evaluated. The functionality of the device was tested with model HeLa cells stably transfected with fluorescent Premo FUCCI Cell Cycle Sensor. The designed device has the potential to be implemented for testing the tendency of patients' tumors to metastasis.
Faculty of Science Masaryk University Brno Czech Republic
Institute of Analytical Chemistry of the Czech Academy of Sciences Brno Czech Republic
Institute of the Biophysics of the Czech Academy of Sciences Brno Czech Republic
Zobrazit více v PubMed
Mayor, R., Etienne-Manneville, S., Nat. Rev. Mol. Cell Biol. 2016, 17, 97-109.
Paul, C. D., Mistriotis, P., Konstantopoulos, K., Nat. Rev. Cancer 2017, 17, 131-140.
Wolf, K., te Lindert, M., Krause, M., Alexander, S., te Riet, J., Willis, A. L., Hoffman, R. M., Figdor, C. G., Weiss, S. J., Friedl, P., J. Cell Biol. 2013, 201, 1069-1084.
Shankar, J., Messenberg, A., Chan, J., Underhill, T. M., Foster, L. J., Nabi, I. R., Cancer Res. 2010, 70, 3780-3790.
Wyckoff, J. B., Pinner, S. E., Gschmeissner, S., Condeelis, J. S., Sahai, E., Curr. Biol. 2006, 16, 1515-1523.
Roussos, E. T., Condeelis, J. S., Patsialou, A., Nat. Rev. Cancer 2011, 11, 573-587.
Kramer, N., Walzl, A., Unger, C., Rosner, M., Krupitza, G., Hengstschlager, M., Dolznig, H., Mutat. Res. 2013, 752, 10-24.
Somaweera, H., Ibraguimov, A., Pappas, D., Anal. Chim. Acta 2016, 907, 7-17.
Kovarik, M. L., Brown, P. J. B., Kysela, D. T., Berne, C., Kinsella, A. C., Brun, Y. V., Jacobson, S. C., Anal. Chem. 2010, 82, 9357-9364.
Mosadegh, B., Lockett, M. R., Minn, K. T., Simon, K. A., Gilbert, K., Hillier, S., Newsome, D., Li, H., Hall, A. B., Boucher, D. M., Eustace, B. K., Whitesides, G. M., Biomaterials 2015, 52, 262-271.
Zhao, S. J., Gao, R. C., Devreotes, P. N., Mogilner, A., Zhao, M., Cell Biol. Int. 2013, 37, 995-1002.
Vasaturo, A., Caserta, S., Russo, I., Preziosi, V., Ciacci, C., Guido, S., PLoS One 2012, 7.
Chaw, K. C., Manimaran, M., Tay, E. H., Swaminathan, S., Lab Chip 2007, 7, 1041-1047.
Denais, C. M., Gilbert, R. M., Isermann, P., McGregor, A. L., te Lindert, M., Weigelin, B., Davidson, P. M., Friedl, P., Wolf, K., Lammerding, J., Science 2016, 352, 353-358.
Davidson, P. M., Sliz, J., Isermann, P., Denais, C., Lammerding, J., Integr. Biol. 2015, 7, 1534-1546.
Davidson, P. M., Denais, C., Bakshi, M. C., Lammerding, J., Cell. Mol. Bioeng. 2014, 7, 293-306.
Elacqua, J. J., McGregor, A. L., Lammerding, J., PLoS One 2018, 13, e0195664.
Krause, M., Yang, F. W., te Lindert, M., Isermann, P., Schepens, J., Maas, R. J. A., Eid, K., Venkataraman, C., Lammerding, J., Madzvamuse, A., Hendriks, W., te Riet, J., Wolf, K., Philos. Trans. R. Soc. B 2019, 374, 20180225.
Sakaue-Sawano, A., Kurokawa, H., Morimura, T., Hanyu, A., Hama, H., Osawa, H., Kashiwagi, S., Fukami, K., Miyata, T., Miyoshi, H., Imamura, T., Ogawa, M., Masai, H., Miyawaki, A., Cell 2008, 132, 487-498.
Suchankova, J., Kozubek, S., Legartova, S., Sehnalova, P., Kuntziger, T., Bartova, E., Biol. Cell 2015, 107, 440-454.
Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., Tinevez, J. Y., White, D. J., Hartenstein, V., Eliceiri, K., Tomancak, P., Cardona, A., Nat. Methods 2012, 9, 676-682.
Ngalim, S. H., Magenau, A., Zhu, Y., Tonnesen, L., Fairjones, Z., Gooding, J. J., Bocking, T., Gaus, K., J. Vis. Exp. 2013, 74, e50310.
Zhang, L. J., Wang, T. T., Wen, X. M., Wei, Y., Peng, X. C., Li, H., Wei, L., Eur. J. Pharmacol. 2007, 563, 69-76.
Walker, G. M., Sai, J. Q., Richmond, A., Stremler, M., Chung, C. Y., Wikswo, J. P., Lab Chip 2005, 5, 611-618.
Moazzam, F., DeLano, F. A., Zweifach, B. W., SchmidSchonbein, G. W., Proc. Natl. Acad. Sci. USA 1997, 94, 5338-5343.
Fukuda, S., Schmid-Schonbein, G. W., Proc. Natl. Acad. Sci. USA 2003, 100, 13152-13157.
