Microfluidic device for concentration and SERS-based detection of bacteria in drinking water
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Bacteria, Drinking water, Microfluidics, Porous membrane, Surface-enhanced Raman spectrometry,
- MeSH
- Equipment Design MeSH
- Metal Nanoparticles chemistry MeSH
- Lab-On-A-Chip Devices * MeSH
- Microfluidic Analytical Techniques instrumentation MeSH
- Drinking Water microbiology MeSH
- Spectrum Analysis, Raman instrumentation MeSH
- Silver chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Drinking Water MeSH
- Silver MeSH
There is a constant need for the development of easy-to-operate systems for the rapid and unambiguous identification of bacterial pathogens in drinking water without the requirement for time-consuming culture processes. In this study, we present a disposable and low-cost lab-on-a-chip device utilizing a nanoporous membrane, which connects two stacked perpendicular microfluidic channels. Whereas one of the channels supplies the sample, the second one attracts it by potential-driven forces. Surface-enhanced Raman spectrometry (SERS) is employed as a reliable detection method for bacteria identification. To gain the effect of surface enhancement, silver nanoparticles were added to the sample. The pores of the membrane act as a filter trapping the bodies of microorganisms as well as clusters of nanoparticles creating suitable conditions for sensitive SERS detection. Therein, we focused on the construction and characterization of the device performance. To demonstrate the functionality of the microfluidic chip, we analyzed common pathogens (Escherichia coli DH5α and Pseudomonas taiwanensis VLB120) from spiked tap water using the optimized experimental parameters. The obtained results confirmed our system to be promising for the construction of a disposable optical platform for reliable and rapid pathogen detection which couples their electrokinetic concentration on the integrated nanoporous membrane with SERS detection.
Department Solar Materials Helmholtz Centre for Environmental Research GmbH Leipzig Germany
Institute of Analytical Chemistry Czech Academy of Sciences Brno Czech Republic
Institute of Analytical Chemistry Leipzig University Leipzig Germany
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