MALDI Mass Spectrometry Imaging of Lipids on Free-Floating Brain Sections and Immunohistochemically Colocalized Markers of Neurodegeneration
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Colocalization, Free-floating, Immunohistochemistry, MALDI MSI, Multimodal, Tissue section,
- MeSH
- Biomarkers MeSH
- Diagnostic Imaging MeSH
- Immunohistochemistry MeSH
- Lipids MeSH
- Brain * diagnostic imaging MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biomarkers MeSH
- Lipids MeSH
In mass spectrometry imaging (MSI), the essential steps in sample preparation include collection and storage. The most widely used preservation procedure for MSI consists in freezing samples and storing them at temperatures below -80 °C. On the other hand, the most common method for preserving biological samples in clinical practice is their fixation in paraformaldehyde. The storage of free-floating sections is a particular type of the preservation of paraformaldehyde-fixed tissues that is used in immunohistochemistry. This chapter describes the approach of the multimodal imaging of free-floating brain sections using the MSI of lipids and the immunohistochemistry of neurodegeneration markers.
See more in PubMed
Buchberger AR, DeLaney K, Johnson J, Li L (2018) Mass spectrometry imaging: a review of emerging advancements and future insights. Anal Chem 90(1):240–265 DOI
Goodwin RJA (2012) Sample preparation for mass spectrometry imaging: small mistakes can lead to big consequences. J Proteome 75(16):4893–4911 DOI
Norris JL, Caprioli RM (2013) Analysis of tissue specimens by matrix-assisted laser desorption/ionization imaging mass spectrometry in biological and clinical research. Chem Rev 113(4):2309–2342 DOI
Schwartz SA, Reyzer ML, Caprioli RM (2003) Direct tissue analysis using matrix-assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation. J Mass Spectrom 38(7):699–708 DOI
Werner M, Chott A, Fabiano A, Battifora H (2000) Effect of formalin tissue fixation and processing on immunohistochemistry. Am J Surg Pathol 24(7):1016–1019 DOI
Pietrowska M, Gawin M, Polańska J, Widłak P (2016) Tissue fixed with formalin and processed without paraffin embedding is suitable for imaging of both peptides and lipids by MALDI-IMS. Proteomics 16(11-12):1670–1677 DOI
Casadonte R, Kriegsmann M, Zweynert F, Friedrich K, Bretton G, Otto M, Deininger S-O, Paape R, Belau E, Suckau D, Aust D, Pilarsky C, Kriegsmann J (2014) Imaging mass spectrometry to discriminate breast from pancreatic cancer metastasis in formalin-fixed paraffin-embedded tissues. Proteomics 14(7-8):956–964 DOI
Groseclose MR, Massion PP, Chaurand P, Caprioli RM (2008) High-throughput proteomic analysis of formalin-fixed paraffin-embedded tissue microarrays using MALDI imaging mass spectrometry. Proteomics 8(18):3715–3724 DOI
Lemaire R, Desmons A, Tabet JC, Day R, Salzet M, Fournier I (2007) Direct analysis and MALDI imaging of formalin-fixed, paraffin-embedded tissue sections. J Proteome Res 6(4):1295–1305 DOI
Carter CL, McLeod CW, Bunch J (2011) Imaging of phospholipids in formalin fixed rat brain sections by matrix assisted laser desorption/ionization mass spectrometry. J Am Soc Mass Spectrom 22(11):1991 DOI
Harris A, Roseborough A, Mor R, Yeung KKC, Whitehead SN (2020) Ganglioside detection from formalin-fixed human brain tissue utilizing MALDI imaging mass spectrometry. J Am Soc Mass Spectrom 31(3):479–487 DOI
Potts EM, Coppotelli G, Ross JM (2020) Histological-based Stainings using free-floating tissue sections. J Vis Exp (162)
Bachman J (2013) Immunohistochemistry on freely floating fixed tissue sections. Methods Enzymol 533:207–215 DOI
Strnad Š, Pražienková V, Holubová M, Sýkora D, Cvačka J, Maletínská L, Železná B, Kuneš J, Vrkoslav V (2020) Mass spectrometry imaging of free-floating brain sections detects pathological lipid distribution in a mouse model of Alzheimer's-like pathology. Analyst 145(13):4595–4605 DOI
Stoeckli M, Staab D, Wetzel M, Brechbuehl M (2014) iMatrixSpray: a free and open source sample preparation device for mass spectrometric imaging. Chimia (Aarau) 68(3):146–149 DOI
Strnad Š, Pražienková V, Sýkora D, Cvačka J, Maletínská L, Popelová A, Vrkoslav V (2019) The use of 1,5-diaminonaphthalene for matrix-assisted laser desorption/ionization mass spectrometry imaging of brain in neurodegenerative disorders. Talanta 201:364–372 DOI
Sládková K, Houška J, Havel J (2009) Laser desorption ionization of red phosphorus clusters and their use for mass calibration in time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 23(19):3114–3118 DOI
