We report an outbreak of SARS-CoV-2 lineage alpha in gorillas and felid species in a zoo in Prague, Czech Republic. The course of illness and clinical signs are described, as are the results of characterization of these particular SARS-CoV-2 variants by next-generation sequencing and phylogenetic analysis. The putative transmission routes are also discussed.

National Institute of Public Health Prague Czech Republic

Prague Zoo Prague Czech Republic

State Veterinary Institute Prague Prague Czech Republic

State Veterinary Institute Prague Sídlištní 136 24 165 03 Prague 6 Czech Republic

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