Submucosal injection is often required step during endoscopic mucosal resection (EMR). In clinical practice we have observed that the EMR injection solution containing hetastarch (HES) lead to selective increase of the neoplasms volume, facilitating their resection. The aim of this study was to explore the possible mechanisms of such behaviour, which was not reported elsewhere. The HCT116 cell line of human colon cancer was exposed to the same EMR solution in vitro. The significant volume increase of HCT116 cells was observed, but only for starving cell culture, suggesting that the starving is essential for the neoplasms-specific volume change. We suggest, that for the iso-oncotic composition of the EMR submucosa injection solution the HES component is crucial, as it can be subject of the starch hydrolysis followed by facilitated transport of resulting monosaccharides from the submucosa into the neoplastic tissue.

Department of Biophysics Institute of Physics Faculty of Science Pavol Jozef Šafárik University in Košice Jesenná 5 040 01 Košice Slovakia

Department of Gastroenterology Central Military Hospital Faculty Hospital Ružomberok Slovakia

Department of Pharmacology Faculty of Medicine Pavol Jozef Šafárik University in Košice Košice Slovakia

Department of Social and Clinical Pharmacy Faculty of Pharmacy in Hradec Králové Charles University Prague Czech Republic

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