Preparative protein concentration from biofluids by epitachophoresis
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
36323110
DOI
10.1016/j.chroma.2022.463591
PII: S0021-9673(22)00782-8
Knihovny.cz E-resources
- Keywords
- Concentration, Epitachophoresis, Growth medium, Proteins, Urine,
- MeSH
- Cytochromes c MeSH
- HeLa Cells MeSH
- Isotachophoresis * methods MeSH
- Humans MeSH
- Proteins MeSH
- Body Fluids * MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Cytochromes c MeSH
- Proteins MeSH
With increasing demands on protein analyses in complex biological matrices, the insistence on developing new sample preparation techniques is rising. Recently, we introduced a new displacement electrophoresis technique (epitachophoresis) and instrumentation for preparative concentration and cleaning of DNA samples. This work describes the possibility of applying this device to protein samples. We have developed a method for the epitachophoretic concentration of proteins in a cationic mode and tested it by concentrating and collecting the protein zones from complex biological matrices (urine and growth medium). Under optimized conditions, we have obtained recoveries up to 99%. Furthermore, the applicability of the developed method was proven by concentrating and collecting the cytochrome c zone from a HeLa cell line growth medium, where the protein cytochrome c was released during cell apoptosis.
Czech Academy of Sciences Institute of Analytical Chemistry Veveří 967 97 Brno 602 00 Czech Republic
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