Myh6 promoter-driven Cre recombinase excises floxed DNA fragments in a subset of male germline cells
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem
Grantová podpora
R01 HL102368
NHLBI NIH HHS - United States
R01 HL146810
NHLBI NIH HHS - United States
Odkazy
PubMed
36584478
PubMed Central
PMC9974737
DOI
10.1016/j.yjmcc.2022.12.005
PII: S0022-2828(22)00588-0
Knihovny.cz E-zdroje
- Klíčová slova
- Cre, Germline cells, Myh6 promoter, tdTom reporter,
- MeSH
- integrasy * genetika metabolismus MeSH
- myši transgenní MeSH
- myši MeSH
- promotorové oblasti (genetika) genetika MeSH
- zárodečné buňky * metabolismus MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
- Názvy látek
- Cre recombinase MeSH Prohlížeč
- integrasy * MeSH
Myh6-Cre transgenic mouse line was known to express Cre recombinase only in the heart. Nevertheless, during breeding Myh6-Cre to Rosa26fstdTom reporter (tdTom) mouse line, we observed that a significant part of their F2 tdTom/+ offspring had tdTom reporter gene universally activated. Our results show that Myh6-Cre transgenic mice have Cre recombinase activity in a subpopulation of the male germline cells, and that Myh6 gene transcripts are enriched in the interstitial Leydig cells and the undifferentiated spermatogonia stem cells. In summary, the current study confirms that the previously known "heart-specific" Myh6 promoter drives Cre expression in the testis.
Department of Human Genetics University of Michigan Ann Arbor MI USA
Masonic Medical Research Institute 2150 Bleecker Street Utica NY 13501 United States of America
Houda A, Nyaz S, Sobhy BM, Bosilah AH, Romeo M, Michael JP et al. (2021). Seminiferous Tubules and Spermatogenesis. In: Male Reproductive Anatomy eds.), IntechOpen,