Raman imaging monitors the time-resolved response of A. thaliana to the artificial inhibition of PSII
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
36623348
DOI
10.1016/j.saa.2022.122276
PII: S1386-1425(22)01424-X
Knihovny.cz E-zdroje
- Klíčová slova
- Arabidopsis thaliana, Bioimaging, Light stress, Phenotyping, Raman spectrometry, Repressor of silencing,
- MeSH
- Arabidopsis * metabolismus MeSH
- chlorofyl metabolismus MeSH
- fotosystém II (proteinový komplex) * antagonisté a inhibitory MeSH
- karotenoidy metabolismus MeSH
- listy rostlin metabolismus MeSH
- protoonkogenní proteiny metabolismus MeSH
- Ramanova spektroskopie MeSH
- světlo MeSH
- tyrosinkinasy metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chlorofyl MeSH
- fotosystém II (proteinový komplex) * MeSH
- karotenoidy MeSH
- protoonkogenní proteiny MeSH
- tyrosinkinasy MeSH
The short-term (0-96 h) response of A. thaliana to the oxidative stress induced by PSII inhibitor metribuzin was examined using Raman spectroscopy. Whole leaves of wildtype (WT, Col-0) and ros1 mutant were scanned and changes in carotenoids were examined. Strong differences in Raman intensity distributions between WT and ros1 were observed. A stronger decrease of carotenoid v1(C=C) band intensity across the leaf was observed in ros1 after 48 h of exposure to metribuzin. It can be assumed that higher sensitivity to oxidative stress in ros1 mutant results in significantly faster degradation of carotenoids.
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