An LRR receptor kinase controls ABC transporter substrate preferences during plant growth-defense decisions
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
37146609
DOI
10.1016/j.cub.2023.04.029
PII: S0960-9822(23)00477-3
Knihovny.cz E-resources
- Keywords
- ABCG36, Arabidopsis thaliana, Botrytis cinerea, Fusarium oxysporum, IBA, PDR8, PEN3, Phytophtora infestans, QSK1, auxin, camalexin, defense, growth,
- MeSH
- ATP-Binding Cassette Transporters metabolism MeSH
- Arabidopsis * metabolism MeSH
- Phytoalexins MeSH
- Plant Diseases microbiology MeSH
- Arabidopsis Proteins * metabolism MeSH
- Gene Expression Regulation, Plant MeSH
- Thiazoles metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- ATP-Binding Cassette Transporters MeSH
- camalexin MeSH Browser
- Phytoalexins MeSH
- PDR8 protein, Arabidopsis MeSH Browser
- Arabidopsis Proteins * MeSH
- Thiazoles MeSH
The exporter of the auxin precursor indole-3-butyric acid (IBA), ABCG36/PDR8/PEN3, from the model plant Arabidopsis has recently been proposed to also function in the transport of the phytoalexin camalexin. Based on these bonafide substrates, it has been suggested that ABCG36 functions at the interface between growth and defense. Here, we provide evidence that ABCG36 catalyzes the direct, ATP-dependent export of camalexin across the plasma membrane. We identify the leucine-rich repeat receptor kinase, QIAN SHOU KINASE1 (QSK1), as a functional kinase that physically interacts with and phosphorylates ABCG36. Phosphorylation of ABCG36 by QSK1 unilaterally represses IBA export, allowing camalexin export by ABCG36 conferring pathogen resistance. As a consequence, phospho-dead mutants of ABCG36, as well as qsk1 and abcg36 alleles, are hypersensitive to infection with the root pathogen Fusarium oxysporum, caused by elevated fungal progression. Our findings indicate a direct regulatory circuit between a receptor kinase and an ABC transporter that functions to control transporter substrate preference during plant growth and defense balance decisions.
College of Life Sciences Ritsumeikan University Shiga 525 8577 Japan
Department of Biology ETH Zurich Universitätstrasse 2 8092 Zurich Switzerland
Department of Biology University of Fribourg 1700 Fribourg Switzerland
References provided by Crossref.org
HSP90 differentially stabilizes plant ABCB-type auxin transporters on the plasma membrane
