Scoring thermal limits in small insects using open-source, computer-assisted motion detection
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't
Grant support
1010996
National Institute of Food and Agriculture
1826689
Office of Integrative Activities
1010996, 2017-33522-27068
National Institute of Food and Agriculture
OIA-1826689
National Science Foundation
CZ.02.2.69/0.0/0.0/18_053/0016979
Ministry of Education, Youth and Sports
PubMed
37902137
DOI
10.1242/jeb.246548
PII: 334476
Knihovny.cz E-resources
- Keywords
- Automated particle tracking, Automatic scoring, Bioassay, Motor performance, Thermal limits,
- MeSH
- Acclimatization * MeSH
- Insecta MeSH
- Computers MeSH
- Motion MeSH
- Software * MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
Scoring thermal tolerance traits live or with recorded video can be time consuming and susceptible to observer bias, and as with many physiological measurements, there can be trade-offs between accuracy and throughput. Recent studies show that automated particle tracking is a viable alternative to manually scoring videos, although some of the software options are proprietary and costly. In this study, we present a novel strategy for automated scoring of thermal tolerance videos by inferring motor activity with motion detection using an open-source Python command line application called DIME (detector of insect motion endpoint). We apply our strategy to both dynamic and static thermal tolerance assays, and our results indicate that DIME can accurately measure thermal acclimation responses, generally agrees with visual estimates of thermal limits, and can significantly increase throughput over manual methods.
Department of Entomology University of Kentucky Lexington KY 40508 USA
Department of Mechanical Engineering University of Kentucky Lexington KY 40508 USA
References provided by Crossref.org
Dryad
10.5061/dryad.cfxpnvxc2
