Probiotic bacteria are widely used in pharmaceutics to offer health benefits. Microencapsulation is used to deliver probiotics into the human body. Capsules in the stomach have to keep bacteria constrained until release occurs in the intestine. Once outside, bacteria must maintain enough motility to reach the intestine walls. Here, we develop a platform based on two label-free optical modules for rapidly screening and ranking probiotic candidates in the laboratory. Bio-speckle dynamics assay tests the microencapsulation effectiveness by simulating the gastrointestinal transit. Then, a digital holographic microscope 3D-tracks their motility profiles at a single element level to rank the strains.

Department of Optics Faculty of Science Palacky University 17 listopadu 12 Olomouc 77146 Czechia

Dipartimento di Ingegneria Chimica dei Materiali e della Produzione Industriale Università degli Studi di Napoli Federico 2 Piazzale Vincenzo Tecchio 80 Napoli 80125 Italy

Institute of Applied Sciences and Intelligent Systems E Caianiello National Research Council Via Campi Flegrei 34 Pozzuoli 80078 Italy

Istituto di Scienze dell'Alimentazione Consiglio Nazionale delle Ricerche Via Roma 64 Avellino 83100 Italy

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