Intricate balance of dually-localized catalase modulates infectivity of Leptomonas seymouri (Kinetoplastea: Trypanosomatidae)
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
38663543
DOI
10.1016/j.ijpara.2024.04.007
PII: S0020-7519(24)00077-8
Knihovny.cz E-resources
- Keywords
- Catalase, Hydrogen peroxide, Trypanosomatids,
- MeSH
- Cytoplasm MeSH
- Catalase * metabolism MeSH
- Microbodies metabolism MeSH
- Hydrogen Peroxide * metabolism MeSH
- Trypanosomatina * enzymology genetics MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Catalase * MeSH
- Hydrogen Peroxide * MeSH
Nearly all aerobic organisms are equipped with catalases, powerful enzymes scavenging hydrogen peroxide and facilitating defense against harmful reactive oxygen species. In trypanosomatids, this enzyme was not present in the common ancestor, yet it had been independently acquired by different lineages of monoxenous trypanosomatids from different bacteria at least three times. This observation posited an obvious question: why was catalase so "sought after" if many trypanosomatid groups do just fine without it? In this work, we analyzed subcellular localization and function of catalase in Leptomonas seymouri. We demonstrated that this enzyme is present in the cytoplasm and a subset of glycosomes, and that its cytoplasmic retention is H2O2-dependent. The ablation of catalase in this parasite is not detrimental in vivo, while its overexpression resulted in a substantially higher parasite load in the experimental infection of Dysdercus peruvianus. We propose that the capacity of studied flagellates to modulate the catalase activity in the midgut of its insect host facilitates their development and protects them from oxidative damage at elevated temperatures.
Biotechnology Applied to Pathogens Instituto René Rachou Fundação Oswaldo Cruz Belo Horizonte Brazil
Department of Biochemistry Faculty of Natural Sciences Comenius University Bratislava Slovakia
Department of Parasitology Faculty of Science Charles University Prague Czechia
Institute of Parasitology Biology Centre Czech Academy of Sciences České Budějovice Czechia
Life Science Research Centre Faculty of Science University of Ostrava Ostrava Czechia
Zoological Institute Russian Academy of Sciences St Petersburg Russia
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