INTRODUCTION: Stress and burnout can negatively affect performance, mental health, and the overall well-being of healthcare workers. The study aims to examine the prevalence of stress and burnout, and investigate links between stress, burnout, mental state, and well-being among healthcare workers in the Czech Republic. METHODS: A cross-sectional survey was conducted in the Czech Republic, focusing on healthcare professionals working in various healthcare settings. A total of 1,064 healthcare workers participated in the study. A standardized questionnaire battery was used, consisting of the Maslach Burnout Inventory (MBI), Perceived Stress Scale (PSS) and Supso-7 measuring mental state. Separate correlation and multiple regression analyses were conducted. RESULTS: 46.24% of the healthcare workers reported high levels of emotional exhaustion, 25.56% reported high levels of depersonalization, 24.15% reported low levels of personal accomplishment, while 11.18% reported high levels of perceived stress. The findings revealed that emotional exhaustion, a core component of burnout, was associated with increased feelings of anxiety and depression. Perceived stress was also linked to anxiety and depression, while personal accomplishment appeared to mitigate depression and support positive psychological well-being. CONCLUSION: The study provides promising evidence suggesting that addressing stress and emotional exhaustion, while fostering a sense of personal achievement, could lead to improvements in the mental health and work performance of healthcare workers. These findings highlight the importance of addressing burnout and stress management strategies to support the overall well-being of healthcare professionals.

Department of Humanities and Social Sciences Faculty of Health Science Palacký University Olomouc Olomouc Czechia

Department of Nursing Midwifery and Health Faculty of Health and Life Sciences Northumbria University Newcastle upon Tyne United Kingdom

Department of Psychology and Abnormal Psychology Faculty of Education Palacký University Olomouc Olomouc Czechia

Department of Psychology Faculty of Arts Palacký University Olomouc Olomouc Czechia

Science and Research Centre Faculty of Health Science Palacký University Olomouc Olomouc Czechia

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