Significant hemolysis is present during irreversible electroporation of cardiomyocytes in vitro
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
39147303
DOI
10.1016/j.hrthm.2024.08.019
PII: S1547-5271(24)03138-2
Knihovny.cz E-resources
- Keywords
- Atrial fibrillation, Cardiomyocytes, Hemolysis, Irreversible electroporation, Pulsed field ablation,
- MeSH
- Electroporation * methods MeSH
- Atrial Fibrillation * surgery MeSH
- Hemolysis * MeSH
- Myocytes, Cardiac * pathology MeSH
- Catheter Ablation * methods adverse effects MeSH
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
BACKGROUND: Pulsed field ablation (PFA) of atrial fibrillation is a new method in clinical practice. Despite a favorable safety profile of PFA in atrial fibrillation ablation, rare cases of renal failure, probably due to hemolysis, have recently been reported. OBJECTIVE: The aim of this study was to determine the rate of hemolysis and cardiac cell death during in vitro PFA with different electric field intensities. METHODS: Blood samples from healthy volunteers and mouse HL-1 cardiomyocyte cell lines were subjected to in vitro irreversible electroporation using 216 bipolar pulses, each lasting 2 μs with intervals of 5 μs, repeated 20 times at a frequency of 1 Hz. These pulses varied from 500 V to 1500 V. Cell-free hemoglobin levels were assessed spectrophotometrically, and red blood cell microparticles were evaluated by flow cytometry. Cardiomyocyte death was quantified with propidium iodide. RESULTS: Pulsed field energy (1000 V/cm, 1250 V/cm, and 1500 V/cm) was associated with a significant increase in cell-free hemoglobin (0.32 ± 0.16 g/L, 2.2 ± 0.96 g/L, and 5.7 ± 0.39 g/L; P < .01) and similar increase in the concentration of red blood cell microparticles. Significant rates of cardiomyocyte death were observed at electric field strengths of 750 V/cm, 1000 V/cm, 1250 V/cm, and 1500 V/cm (26.5% ± 5.9%, 44.3% ± 6.2%, 55.5% ± 6.9%, and 74.5% ± 17.8% of cardiomyocytes; P < .01). CONCLUSION: The most effective induction of cell death in vitro was observed at 1500 V/cm. This intensity was also associated with a significant degree of hemolysis.
Department of Biochemistry Cell and Molecular Biology Charles University Prague Czech Republic
Institute for Biostatistical Analyses Masaryk University Brno Czech Republic
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