Bridging basic science and applied diagnostics: Comprehensive viral diagnostics enabled by graphene-based electronic biosensor technology advancements
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
Grant support
P 35103
Austrian Science Fund FWF - Austria
PubMed
39341071
DOI
10.1016/j.bios.2024.116807
PII: S0956-5663(24)00813-3
Knihovny.cz E-resources
- Keywords
- COVID-19, Diagnostics, Graphene field-effect transistor, Infectivity, Nucleocapsid protein, SARS-CoV-2,
- MeSH
- Biosensing Techniques * instrumentation methods MeSH
- COVID-19 * diagnosis virology MeSH
- Equipment Design MeSH
- Transistors, Electronic MeSH
- Phosphoproteins MeSH
- Graphite * chemistry MeSH
- Coronavirus Nucleocapsid Proteins isolation & purification MeSH
- Humans MeSH
- Limit of Detection MeSH
- Nasopharynx virology MeSH
- RNA, Viral * isolation & purification analysis MeSH
- SARS-CoV-2 * isolation & purification genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Phosphoproteins MeSH
- Graphite * MeSH
- Coronavirus Nucleocapsid Proteins MeSH
- nucleocapsid phosphoprotein, SARS-CoV-2 MeSH Browser
- RNA, Viral * MeSH
This study presents a graphene field-effect transistor (gFET) biosensor with dual detection capabilities for SARS-CoV-2: one RNA detection assay to confirm viral positivity and the other for nucleocapsid (N-)protein detection as a proxy for infectiousness of the patient. This technology can be rapidly adapted to emerging infectious diseases, making an essential tool to contain future pandemics. To detect viral RNA, the highly conserved E-gene of the virus was targeted, allowing for the determination of SARS-CoV-2 presence or absence using nasopharyngeal swab samples. For N-protein detection, specific antibodies were used. Tested on 213 clinical nasopharyngeal samples, the gFET biosensor showed good correlation with RT-PCR cycle threshold values, proving its high sensitivity in detecting SARS-CoV-2 RNA. Specificity was confirmed using 21 pre-pandemic samples positive for other respiratory viruses. The gFET biosensor had a limit of detection (LOD) for N-protein of 0.9 pM, establishing a foundation for the development of a sensitive tool for monitoring active viral infection. Results of gFET based N-protein detection corresponded to the results of virus culture in all 16 available clinical samples and thus it also proved its capability to serve as a proxy for infectivity. Overall, these findings support the potential of the gFET biosensor as a point-of-care device for rapid diagnosis of SARS-CoV-2 infection and indirect assessment of infectiousness in patients, providing additional information for clinical and public health decision-making.
BioSensor Technologies Austrian Institute of Technology Vienna Austria
Department of Biotechnology BOKU University Vienna Austria
Department of Urology Medical University of Vienna Vienna Austria
Institute Krems Bioanalytics IMC Krems University of Applied Sciences Krems Austria
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