A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.

DUI

D022521 MeSH Prohlížeč

CUI

M0358403

Předchozí užití

Gene Amplification (1980-2000)

Historická pozn.

2001

Veřejná pozn.

2001