In our clinic we performed in the period 1999-2008 more than 4000 sperm assays. Obtained values were analysed and they were compared with related IVF/ICSI cycles outcome. The 1738 related IVF/ICSI cycles were used for the assisted reproduction outcome analysis, in 1395 of them was treated by ICSI alone, 215 by IVF alone, and 128 with both methods. Material and methods: In concordance with WHO criteria, the volume, concentration, motility, morphology (using strict criteria in stained smears), total sperm count, total progressive motile sperm count, and total count of sperm with normal morphology were evaluated. They were parsed in the aspect of age, the cross correlation of above mentioned criteria, and the IVF and ICSI outcome (fertilization rate, embryo in vitro development, pregnancy rate, abortion rate). Results: Sperm assays of 3345 men were carried out. The mean age was 32.6 years (16.9 to 76.9 years), the sperm concentration 68.0 millions per ml (0 to 560 millions per ml), motility A 26.5% (0 to 93%), motility B 27.6% (0 to 93%), normal morphology 20.9% (0 to 100%). The 3% of men were azoospermic and 0.75% cryptozoospermic. The concentration, motility, and sperm morphology was age dependent with some maxima and minima, with the apparent decline in the group from 56 to 77 years old men. The fertilization rate, in vitro development, pregnancy rate and abortion rate were found to partially correlate with the sperm parameters. Conclusions: We found that the distribution of sperm values is rather logarithmic than normal, and set of examined men probably creates several independent subsets. The average sperm concentration, motility, and morphology exceed in our file the WHO criteria. The assisted reproduction outcome correlates only partially with the sperm parameters.
- MeSH
- hodnotící studie jako téma MeSH
- motilita spermií MeSH
- odběr spermií využití MeSH
- počet spermií MeSH
- preimplantační diagnóza využití MeSH
- spermie cytologie MeSH
- Check Tag
- mužské pohlaví MeSH
- Publikační typ
- abstrakty MeSH
Since 1994, when the first paper was published by Harper and Handyside summarizing the world experience of PGD, this method became a routine procedure both in precise diagnostic and therapy of infertility. The vast majority of PGD cycles for the chromosomal abnormity are performed with the major indication being aneuploidy. Nevertheless the number of PGD cycles for structural rearrangements are also growing up. Methods: The occurrence of non-disjunction during meiosis is a quite rare event in most organisms with the exception of the human species where approximately a half of oocytes and a tenth of spermatozoa carry chromosomal abnormity. In addition, other possible causes of chromosomal aneuploidy are considered, e.g. the dysfunction of the spindle during early mitosis. Results: The group of indications for PGD and PGD-AS (aneuploidy screening) is dynamically expanding, and the growing experience in PGD is generating a daunting myriad of innovations in assisted reproductive techniques and turns dramatically the angle of a view to a general concept on human infertility. Conclusions: The strengthening tendency to enlargement the numbers of PGD cycles is awaited for the future decade with the respect to the expected benefits for the clients. New methods simplifying the PGD diagnosis are considered and welcome. 19
Preimplantation genetic diagnosis (PGD) abandoned the group of experimental methods and became an unreplaceable and indespensable therapeutic procedure in selected group of infertile couples. Methods: The authors reffer the group of 58 PGD cycles for the male infertility factor performed since 2004 to 2005, with the major reason being teratospermia. The control group was created of 67 cycles for PGD-AS. Fluorescent in - situ hybridization (FISH) was used to detect embryo aneuploidy (up to 8 chromosomes). Other conditions and procedures did not differ in any respect. Results: More than 60 % of preimplantation embryos in MFI group carried chromosomal abnormalities. Both the blastocyst embryotransfers ratio (65 % and 78 %) and pregnancy rates per embryotransfer (19 % and 32 %) were significantly lower compared to the control group. Conclusions: Recent advances in PGD, and the widening success to cure infertility even in couples with poor fertility prognosis bring a positive outlook for improving the results in the whole field of reproductive medicine and genetics. The future focusation for the mail factor of infertility with respect to teratospermia is essential. Number of questions arising on the MFI might lead to designing next clinical studies.
- MeSH
- aneuploidie MeSH
- asistovaná reprodukce MeSH
- preimplantační diagnóza metody MeSH
- Publikační typ
- kongresy MeSH
