INTRODUCTION: The use of new psychoactive substances as drugs of abuse has dramatically increased over the last years. Hallucinogenic phenethylamines gained particular popularity as they have both stimulating and psychedelic effects. Although generally perceived as safe, these illicit drugs pose a serious health risk; they have been linked to cases of severe poisoning or even deaths. Therefore, simple, cost-effective and reliable methods are needed for rapid determination of abused hallucinogens. METHODS: For this purpose, two haptens derived from 2C-H were designed, synthesized and subsequently attached to a carrier protein. Polyclonal antibodies obtained from a rabbit immunized with one of the prepared immunogens were used for the development of two immunoassays. RESULTS: In this study, a lateral flow immunoassay (LFIA) and an enzyme linked immunosorbent assay (ELISA) for the detection of 2C-B and related hallucinogenic phenethylamines in urine were developed. The presented LFIA is primarily suitable for on-site monitoring as it is simple and can provide a visual evidence of 2C-B presence within a few minutes. Its reasonable sensitivity (LODLFIA = 15 ± 7 ng mL-1) allows detection of the drug presence in urine after acute exposure. For greater accuracy, highly sensitive ELISA (LODELISA = 6 ± 3 pg mL-1) is proposed for toxicological quantitative analyses of positive samples captured by the LFIA. DISCUSSION: The comparison of the ELISA with the well-established UHPLC-MS-MS method shows excellent agreement of results, which confirms good potential of the ELISA to be used for routine analyses of 2C-B and related hallucinogenic phenethylamines of both main sub-families.

• MeSH
• dimethoxyfenylethylamin analogy a deriváty   chemie   imunologie   moč   MeSH
• halucinogeny chemie   imunologie   moč   MeSH
• hapteny chemie   imunologie   MeSH
• imunoanalýza ekonomika   metody   MeSH
• lidé MeSH
• odhalování abúzu drog přístrojové vybavení   metody   MeSH
• reprodukovatelnost výsledků MeSH
• tandemová hmotnostní spektrometrie přístrojové vybavení   metody   MeSH
• zakázané drogy chemie   imunologie   moč   MeSH
• zdraví dobrovolníci pro lékařské studie MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• validační studie MeSH

• MeSH
• chemická stimulace MeSH
• dimethoxyfenylethylamin analýza   chemie   škodlivé účinky   MeSH
• finanční podpora výzkumu jako téma MeSH
• halucinogeny klasifikace   metabolismus   škodlivé účinky   MeSH
• krysa rodu rattus metabolismus   psychologie   MeSH
• lidé MeSH
• LSD chemie   škodlivé účinky   toxicita   MeSH
• N-methyl-3,4-methylendioxyamfetamin analýza   chemie   škodlivé účinky   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus metabolismus   psychologie   MeSH
• lidé MeSH
• zvířata MeSH