Transcatheter Aortic Valve Implantation (TAVI) has revolutionized severe aortic stenosis treatment, but risk stratification remains challenging. This systematic review examined the association between computed tomography (CT)-derived adipose tissue parameters and TAVI outcomes. We searched major databases for studies on visceral (VAT), subcutaneous (SAT), and intramuscular (IMAT) adipose tissue parameters and post-TAVI outcomes. Fourteen studies (9692 patients) were included. Higher SAT area/volume was consistently associated with better survival (5 studies, HR range: 0.83-2.77, p < 0.05). Lower SAT and VAT density also correlated with better survival (5 and 4 studies, respectively, HR range: 1.31-1.46, p < 0.05). VAT area showed mixed results. A VAT:SAT ratio < 1 was associated with better cardiovascular outcomes in one study. Lower IMAT index correlated with shorter hospital stays in a single study. This review reveals complex relationships between adipose tissue parameters and TAVI outcomes. Lower adipose tissue density and higher subcutaneous adiposity were most consistently associated with better outcomes. These findings suggest that detailed analysis of adipose tissue characteristics may enhance risk stratification in TAVI candidates.

• MeSH
• Aortic Valve Stenosis * surgery   mortality   diagnostic imaging   MeSH
• Humans MeSH
• Tomography, X-Ray Computed * methods   MeSH
• Postoperative Complications * etiology   mortality   MeSH
• Transcatheter Aortic Valve Replacement * adverse effects   mortality   MeSH
• Adipose Tissue * diagnostic imaging   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Review MeSH
• Systematic Review MeSH

Nucleotide excision repair (NER) is crucial for repairing bulky lesions and crosslinks in DNA caused by exogenous and endogenous genotoxins. The number of studies that have considered DNA repair as a biomarker is limited, and therefore one of the primary objectives of the European COST Action hCOMET (CA15132) was to assemble and analyse a pooled database of studies with data on NER activity. The database comprised 738 individuals, gathered from 5 laboratories that ran population studies using the comet-based in vitro DNA repair assay. NER activity data in peripheral blood mononuclear cells were normalized and correlated with various host-related factors, including sex, age, body mass index (BMI), and smoking habits. This multifaceted analysis uncovered significantly higher NER activity in female participants compared to males (1.08 ± 0.74 vs. 0.92 ± 0.71; P = .002). Higher NER activity was seen in older subjects (>30 years), and the effect of age was most pronounced in the oldest females, particularly those over 70 years (P = .001). Females with a normal BMI (<25 kg/m2) exhibited the highest levels of NER, whereas the lowest NER was observed in overweight males (BMI ≥ 25 kg/m2). No independent effect of smoking was found. After stratification by sex and BMI, higher NER was observed in smoking males (P = .017). The biological implication of higher or lower repair capacity remains unclear; the inclusion of DNA repair as a biomarker in molecular epidemiological trials should elucidate the link between health and disease status.

• MeSH
• Adult MeSH
• Excision Repair MeSH
• Body Mass Index MeSH
• Comet Assay MeSH
• Smoking MeSH
• Leukocytes, Mononuclear metabolism   MeSH
• Middle Aged MeSH
• Humans MeSH
• DNA Repair * MeSH
• DNA Damage MeSH
• Aged MeSH
• Sex Factors MeSH
• Age Factors MeSH
• Check Tag
• Adult MeSH
• Middle Aged MeSH
• Humans MeSH
• Male MeSH
• Aged MeSH
• Female MeSH
• Publication type
• Journal Article MeSH

Cholinesterases, specifically acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), play critical roles in neurotransmission and are key targets for inhibitors with therapeutic and toxicological significance. This review focuses on the development and application of fluorometric and colorimetric biosensors for the detection of cholinesterase inhibitors. These biosensors take advantage of the unique properties of AChE and BChE to provide sensitive and selective detection methods essential for environmental monitoring, food safety, and clinical diagnostics. Recent advances in assay techniques, including the use of gold nanoparticles, pseudoperoxidase nanomaterials, and innovative enzyme-substrate interactions, are highlighted. This review also discusses challenges and future directions for optimizing these biosensors for practical applications, emphasizing their potential to enhance public health and safety.

• MeSH
• Acetylcholinesterase chemistry   metabolism   MeSH
• Biosensing Techniques * methods   MeSH
• Butyrylcholinesterase chemistry   metabolism   MeSH
• Cholinesterase Inhibitors * analysis   MeSH
• Fluorometry * methods   MeSH
• Colorimetry * methods   MeSH
• Metal Nanoparticles chemistry   MeSH
• Humans MeSH
• Gold chemistry   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Review MeSH

PURPOSE: This study analyzed the kinematics and muscle activity during the stand-up paddleboarding (SUP) under different visual focus points in three conditions: i) eyes on the board nose, ii) looking at the turn buoy, and iii) free choice. METHODS: Fourteen male paddleboarders (24.2 ± 7.1 years) performed three trials covering 65 m, and the electromyographic (EMG) activation patterns and kinematic parameters in four cycle strokes for the left and right sides were analyzed. Surface EMG of the upper trapezius, biceps brachii, triceps brachii, tibialis anterior, and gastrocnemius medialis were recorded. The data were processed according to the percentage of maximum voluntary contraction (%MVC). Speed, stroke frequency (SF), stroke length, and stroke index (SI) were analyzed. RESULTS: The speed, SF, and SI (p < 0.01, η2 ≥ 0.42) showed significant variance between conditions, with the free condition achieving the highest speed (1.20 ± 0.21 m/s), SF (0.65 ± 0.13 Hz) and SI (2.25 ± 0.67 m2/s). This condition showed greater neuromuscular activity, particularly in the triceps brachii during both the left (42.25 ± 18.76 %MVC) and right recoveries (32.93 ± 16.06 %MVC). During the pull phase, the free choice presented higher biceps brachii activity (8.51 ± 2.80 %MVC) compared to the eyes on the board nose (6.22 ± 2.41 %MVC; p < 0.01), while showing lower activity in the triceps brachii (10.02 ± 4.50 %MVC vs. 16.52 ± 8.45 %MVC; p < 0.01) and tibialis anterior (12.24 ± 7.70 %MVC vs. 17.09 ± 7.73 %MVC; p < 0.01) compared to looking at the turn buoy. CONCLUSION: These results suggest that a free visual focus allows paddleboarders to enhance their kinematics and muscle activation, highlighting the significance of visual focus strategies in improving both competitive and recreational SUP performance.

• MeSH
• Biomechanical Phenomena MeSH
• Adult MeSH
• Electromyography MeSH
• Muscle, Skeletal * physiology   MeSH
• Humans MeSH
• Young Adult MeSH
• Muscle Contraction physiology   MeSH
• Check Tag
• Adult MeSH
• Humans MeSH
• Young Adult MeSH
• Male MeSH
• Publication type
• Journal Article MeSH

INTRODUCTION: Sacrococcygeal teratoma (SCT) is the most common congenital tumor. The incidence of malignant types is rare but increases with late detection or in case of relapse. Prenatal diagnosis is based on ultrasound examination and magnetic resonance imaging (MRI). Since this is a rare congenital anomaly, we should report all cases to improve prenatal diagnosis and postnatal management. MATERIAL AND METHODS: Retrospective analysis of sixteen cases of sacrococcygeal teratoma delivered and treated at the University Hospital Brno between 2005 and 2020. The following criteria were evaluated: gestational week of the primary diagnosis, exact description of ultrasound findings, pregnancy management, delivery mode, correlation of prenatal ultrasound with postnatal findings in the newborn, as well as the occurrence of early and late complications in newborns and children. RESULTS: Out of sixteen cases, seven cases (43.8%) were indicated for pregnancy termination based on ultrasound findings, the parent's decision, and an estimation of an adverse pregnancy outcome. In nine cases (56.2%), the pregnancy continued and was ended by delivery. In one case, there was an early postnatal death of a newborn after birth in the 25th week of gestation. In eight cases, live fetuses were born in which the tumor was surgically removed between day 1 and 14 months after birth. There was a strong correlation between the tumor description made by prenatal ultrasound diagnosis and related severe complications in newborns. The incidence of severe early and late complications in ongoing pregnancies was very low-only one case of infection in the surgical wound requiring reoperation (12.5%) was described. In two patients (25%), a transient stoma establishment was necessary for secondary ileus. One case of recurrence of the disease at two years of age occurred, requiring the administration of chemotherapy (12.5%), and one patient has mild persistent urinary incontinence. CONCLUSION: Sacrococcygeal teratoma is one of the rarest congenital malformations. A detailed prenatal ultrasound examination is essential to estimate the pregnancy prognosis. The most predictive ultrasound predictor of favorable early and late postnatal outcomes and long-term child development is the presence of cystic sacrococcygeal formation, the most common tumor type, and the absence of signs of cardiac failure due to fetal anemia. In these cases, with early surgical treatment provided, the incidence of severe complications and long-term consequences in children is very low, and parents should be informed during prenatal counseling. It is necessary to register all the SCT cases due to the rarity of this congenital anomaly for further statistical analysis of the importance of ultrasound markers.

• MeSH
• Adult MeSH
• Gestational Age MeSH
• Humans MeSH
• Magnetic Resonance Imaging MeSH
• Infant, Newborn MeSH
• Prognosis MeSH
• Retrospective Studies MeSH
• Sacrococcygeal Region * diagnostic imaging   MeSH
• Pregnancy MeSH
• Teratoma * diagnostic imaging   surgery   congenital   MeSH
• Ultrasonography, Prenatal * MeSH
• Pregnancy Outcome MeSH
• Check Tag
• Adult MeSH
• Humans MeSH
• Infant, Newborn MeSH
• Pregnancy MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Review MeSH

The aim of this study was to determine prevalence of smokers and ex-smokers in the older diabetes population in Europe and to evaluate the relationship with various sociodemographic and lifestyle risk factors. This epidemiological study used Wave 8 of the multidisciplinary and cross-national SHARE database, which includes cross-sectional data on health, socio-economic status and social and family networks of individuals aged 50 and over from 27 European countries. Among the 6,903 participants with diabetes, 12.2% were current smokers, 29.9% were former smokers and 57.9% had never smoked. Among countries' diabetes populations, the highest prevalence of ex-smokers (57.6%) with a low prevalence of smokers (9.5%) was seen in the Netherlands. The highest prevalence of never-smokers (84.2%) was found in Latvia, with the lowest prevalence of ex-smokers (7.9%). Austria had the highest prevalence of current smokers (17.9%) along with a below-average prevalence of ex-smokers (26.5%) was seen in Austria. Normal weight, being overweight, lower education, living without a partner, living in larger cities, drinking alcohol in the last 7 days, lower age, and being male were associated with a higher risk of being a current smoker. The study shows high prevalence of smoking among the older diabetes population in Europe and provides data on the association of smoking with several risk factors and country-specific differences in smoking prevalence.

• MeSH
• Diabetes Mellitus * epidemiology   MeSH
• Smoking * epidemiology   MeSH
• Middle Aged MeSH
• Humans MeSH
• Smoking Cessation * statistics & numerical data   MeSH
• Prevalence MeSH
• Cross-Sectional Studies MeSH
• Risk Factors MeSH
• Aged, 80 and over MeSH
• Aged MeSH
• Life Style MeSH
• Check Tag
• Middle Aged MeSH
• Humans MeSH
• Male MeSH
• Aged, 80 and over MeSH
• Aged MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Geographicals
• Europe MeSH

Alterations in the excitability of dorsal root ganglion (DRG) neurons are critical in the pathogenesis of acute and chronic pain. Neurotransmitter release from the terminals of DRG neurons is regulated by cannabinoid receptor 1 (CB1) and transient receptor potential vanilloid 1 (TRPV1), both activated by anandamide (AEA). In our experiments, the AEA precursor N-arachidonoylphosphatidylethanolamine (20:4-NAPE) was used to study the modulation of nociceptive DRG neurons excitability using K+-evoked Ca2+ transients. Intrathecal administration was used to evaluate in vivo effects. Application of 20:4-NAPE at lower concentrations (10 nM - 1 μM) decreased the excitability of DRG neurons, whereas the higher (10 μM) increased it. Both effects of 20:4-NAPE were blocked by the N-acylphosphatidylethanolamine phospholipase D (NAPE-PLD) inhibitor LEI-401. Similarly, lower concentrations of externally applied AEA (1 nM - 10 nM) inhibited DRG neurons, whereas higher concentration (100 nM) did not change it. High AEA concentration (10 μM) evoked Ca2+ transients dependent on TRPV1 activation in separate experiments. Inhibition of the CB1 receptor by PF514273 (400 nM) prevented the 20:4-NAPE- and AEA-induced inhibition, whereas TRPV1 inhibition by SB366791 (1 μM) prevented the increased DRG neuron excitability. In behavioral tests, lower 20:4-NAPE concentration caused hyposensitivity, while higher evoked mechanical allodynia. Intrathecal LEI-401 prevented both in vivo effects of 20:4-NAPE. These results highlight anti- and pro-nociceptive effects of 20:4-NAPE mediated by CB1 and TRPV1 in concentration-dependent manner. Our study underscores the complexity of endocannabinoid signaling in pain transmission modulation and highlights 20:4-NAPE as a potential therapeutic target, offering new insights for developing analgesic strategies.

• MeSH
• Endocannabinoids pharmacology   metabolism   MeSH
• Phosphatidylethanolamines * pharmacology   MeSH
• Phospholipase D * metabolism   antagonists & inhibitors   MeSH
• TRPV Cation Channels metabolism   MeSH
• Rats MeSH
• Arachidonic Acids * pharmacology   MeSH
• Neurons * drug effects   metabolism   MeSH
• Polyunsaturated Alkamides pharmacology   MeSH
• Rats, Sprague-Dawley MeSH
• Receptor, Cannabinoid, CB1 metabolism   MeSH
• Ganglia, Spinal * drug effects   metabolism   cytology   MeSH
• Calcium metabolism   MeSH
• Animals MeSH
• Check Tag
• Rats MeSH
• Male MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH

Epilepsy is a neurological disease characterized by epileptic seizures, which commonly manifest with pronounced frequency and amplitude changes in the EEG signal. In the case of focal seizures, initially localized pathological activity spreads from a so-called "onset zone" to a wider network of brain areas. Chimeras, defined as states of simultaneously occurring coherent and incoherent dynamics in symmetrically coupled networks are increasingly invoked for characterization of seizures. In particular, chimera-like states have been observed during the transition from a normal (asynchronous) to a seizure (synchronous) network state. However, chimeras in epilepsy have only been investigated with respect to the varying phases of oscillators. We propose a novel method to capture the characteristic pronounced changes in the recorded EEG amplitude during seizures by estimating chimera-like states directly from the signals in a frequency- and time-resolved manner. We test the method on a publicly available intracranial EEG dataset of 16 patients with focal epilepsy. We show that the proposed measure, titled Amplitude Entropy, is sensitive to the altered brain dynamics during seizure, demonstrating its significant increases during seizure as compared to before and after seizure. This finding is robust across patients, their seizures, and different frequency bands. In the future, Amplitude Entropy could serve not only as a feature for seizure detection, but also help in characterizing amplitude chimeras in other networked systems with characteristic amplitude dynamics.

• MeSH
• Adult MeSH
• Electroencephalography methods   MeSH
• Entropy MeSH
• Epilepsies, Partial * physiopathology   MeSH
• Humans MeSH
• Brain * physiopathology   MeSH
• Seizures * physiopathology   MeSH
• Check Tag
• Adult MeSH
• Humans MeSH
• Male MeSH
• Female MeSH
• Publication type
• Journal Article MeSH

Stuart, A, Vetrovsky, T, Cochrane-Snyman, KC, Vieira, A, and Tufano, JJ. Investigating the consistency in countermovement jump performance after high-velocity and high-force PAPE: A multiday analysis. J Strength Cond Res 39(7): e843-e853, 2025-Postactivation performance enhancement (PAPE) is widely acknowledged in both practical application and research. However, studies have primarily focused on single experimental PAPE sessions, neglecting the repeatability of protocols with the same individuals over time. The purpose of this study was to determine whether PAPE, involving high-velocity and high-force conditioning activities, could be observed at consistent time points across multiple sessions. Sixteen elite speed skaters (9 men: 23.1 ± 2.6 years and 7 women: 24.2 ± 4.7 years) participated in 6 sessions for each independent study. For all sessions, a standardized warm-up was performed, followed by 3 baseline bodyweight countermovement jumps (CMJ), and a potentiation protocol of 5 repeated band-assisted countermovement jumps at approximately 20% bodyweight reduction, or a single 5-second maximal effort isometric squat. Subsequently, subjects rested for 3, 5, and 7 minutes before performing a single bodyweight CMJ at each time point. Multiday analyses of variance showed mixed results for both protocols, with most effect sizes ranging from trivial to small. Linear mixed models indicated that at the individual level, subjects did not consistently jump higher at any specific post-PAPE rest time. The variance between baseline and post-PAPE jump height at the rest time that produced the greatest result was neither statistically significant nor statistically different ( p > 0.05), compared with other rest times. Postactivation performance enhancement was identifiable across sessions but variability in jump performance and inconsistent effects made it difficult to observe repeatable responses within individuals over time, thus questioning the idea of "individualizing" PAPE in practice.

• MeSH
• Skating * physiology   MeSH
• Adult MeSH
• Muscle, Skeletal physiology   MeSH
• Humans MeSH
• Young Adult MeSH
• Plyometric Exercise * MeSH
• Athletic Performance * physiology   MeSH
• Muscle Strength physiology   MeSH
• Warm-Up Exercise physiology   MeSH
• Check Tag
• Adult MeSH
• Humans MeSH
• Young Adult MeSH
• Male MeSH
• Female MeSH
• Publication type
• Journal Article MeSH