BACKGROUND: Hematopoietic stem cell transplantation (HSCT) can induce serious oral complications, including oral mucositis (OM). The presence of periodontal inflammation before HSCT is believed to be associated with OM. The aim of our study was to determine the prevalence and severity of OM in patients undergoing HSCT and its relation to periodontal status. PATIENTS AND METHODS: This is a retrospective study of patients who underwent HSCT and a detailed dental examination between 2007 and 2015. The dental and periodontal status of all patients was evaluated by clinical and radiographic examination prior to HSCT. Oral health was assessed with the gingival index, the the community periodontal index, presence of plaque-related gingivitis, and marginal periodontitis. During the HSCT period, patients were examined daily for the presence of OM, which was graded according to World Health Organization (WHO) classification if present. The patients were assigned to the groups according to type of transplantation: autologous HSCT, myeloablative allogeneic HSCT, and non-myeloablative allogeneic HSCT. RESULTS: A total of 496 patients were included in the study. OM was present in 314 of 496 patients (63.3%): 184/251 (73.3%) in the autologous group, 100/151 (66.2%) in the myeloablative allogeneic group, and 30/94 (31.9%) in the nonmyeloablative allogeneic group. Significantly more patients suffered from OM in the autologous and myeloablative groups versus the nonmyeloablative conditioning group (p < 0.001). The presence of periodontal inflammation did not significantly differ among the groups. There was only a borderline trend for the higher prevalence of OM in the non-myeloablative allogeneic nonmyeloablative group when periodontal inflammation was present (0.073939). CONCLUSIONS: Oral mucositis prevalence and severity after stem cell transplantation is not widely affected by the oral hygiene and periodontal disease presence before HSCT. We confirmed the wide-known connection of the conditioning regimen intensity to the prevalence of OM.

• Publikační typ
• časopisecké články MeSH

Hyaluronic acid (HA) and dental pulp stem cells (DPSCs) are attractive research topics, and their combined use in the field of tissue engineering seems to be very promising. HA is a natural extracellular biopolymer found in various tissues, including dental pulp, and due to its biocompatibility and biodegradability, it is also a suitable scaffold material. However, low molecular weight (LMW) fragments, produced by enzymatic cleavage of HA, have different bioactive properties to high molecular weight (HMW) HA. Thus, the impact of HA must be assessed separately for each molecular weight fraction. In this study, we present the effect of three LMW-HA fragments (800, 1600, and 15,000 Da) on DPSCs in vitro. Discrete biological parameters such as DPSC viability, morphology, and cell surface marker expression were determined. Following treatment with LMW-HA, DPSCs initially presented with an acute reduction in proliferation (p < 0.0016) and soon recovered in subsequent passages. They displayed significant size reduction (p = 0.0078, p = 0.0019, p = 0.0098) while maintaining high expression of DPSC markers (CD29, CD44, CD73, CD90). However, in contrast to controls, a significant phenotypic shift (p < 0.05; CD29, CD34, CD90, CD106, CD117, CD146, CD166) of surface markers was observed. These findings provide a basis for further detailed investigations and present a strong argument for the importance of HA scaffold degradation kinetics analysis.

• MeSH
• buněčná diferenciace účinky léků   MeSH
• kmenové buňky cytologie   účinky léků   MeSH
• kultivované buňky MeSH
• kyselina hyaluronová farmakologie   MeSH
• lidé MeSH
• molekulová hmotnost MeSH
• proliferace buněk účinky léků   MeSH
• tkáňové inženýrství MeSH
• zubní dřeň účinky léků   růst a vývoj   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

INTRODUCTION: Oral manifestations of deficiency of iron, vitamin B12 and folic acid are thought to be common. Prevalence of these deficiencies among patients with compatible symptoms is not well known. The goal of this study was to summarize evidence from a dental practice of iron, vitamin B12 and folic acid deficiency in patients presenting with compatible oral manifestations. METHODS: 250 patients who presented with burning mouth syndrome, angular cheilitis, recurrent aphthous stomatitis, papillar atrophy of the tongue dorsum or mucosal erythema were identified. Patients underwent clinical examination, and the blood samples were taken. RESULTS: 250 patients (208 females; 42 males, mean age 44.1 years) with at least one corresponding symptom or sign were identified. The nutritional deficiency of one or more nutrients was found in 119 patients (47.6%). Seven times more females than males were noted to have one type of deficiency (104 females, 15 males). Iron deficiency as defined was diagnosed in 62 patients (24.8%), vitamin B12 or folic acid deficiency in 44 patients (17.6%) and both deficiencies (iron + vitamin B12/folic acid) in 13 patients (5.2%). The only predictive factor was gender and only for iron deficiency. The presence of more than one deficiency was noted in 10 patients (4.9%). CONCLUSION: The most commonly observed deficiency in dental practice over the course of 11 years was an iron deficiency in the female population. Age, diet and reported co-morbidities did not show statistically significant predictable value in recognizing these deficiencies.

• MeSH
• dospělí MeSH
• kyselina listová krev   MeSH
• lidé MeSH
• nedostatek kyseliny listové * MeSH
• nedostatek vitaminu B12 * MeSH
• nemoci úst * MeSH
• podvýživa MeSH
• sexuální faktory MeSH
• vitamin B 12 krev   MeSH
• vzorové postupy ve stomatologii statistika a číselné údaje   MeSH
• železo * MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH

AIM: The aim of this study was to assess the perception of undergraduate dentistry students at Charles University, Faculty of Medicine in Hradec Králové, the Czech Republic regarding their endodontic education within the context of the Undergraduate Curriculum Guidelines for Endodontology by the European Society of Endodontology (ESE). The secondary aim was to compare this perception among students in the Czech and English groups. METHODOLOGY: A questionnaire survey was conducted among fifth year students at the very end of their studies. RESULTS: The students returned 60 filled questionnaires, making the response rate of 75.9%. More than two thirds of the respondents declared that they were competent at or had knowledge of most of the major competencies defined by the ESE. Eighty seven percent of respondents felt competent to perform a root canal treatment on anterior teeth; 86.7% on premolars; and 48.3% on molars. Nearly all respondents (98.3%) recommended more opportunities to practice on patients. CONCLUSIONS: The overall perception of the students was that their endodontic education was sufficient and largely conformed to the guidelines. Insufficient exposure to endodontic practice on patients was identified as a deficiency. There were no significant differences in perceptions between the two study groups.

• MeSH
• dospělí MeSH
• endodoncie výchova   MeSH
• jazyk (prostředek komunikace) MeSH
• klinické kompetence * MeSH
• kurikulum MeSH
• lidé MeSH
• mladý dospělý MeSH
• percepce MeSH
• průzkumy a dotazníky MeSH
• sebeuplatnění * MeSH
• studenti stomatologie * psychologie   MeSH
• studium stomatologie normy   MeSH
• terapie kořenového kanálku MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH

This article is the second part of an evaluation of musculoskeletal disorders (MSDs) prevalence among dental students. As the majority of complaints are in the back region, there is an endeavor to analyze objectively the disorders in this region. One of the non-invasive and non-radiation methods is the spinal curve mapping using the Spinal Mouse® device (Idiag AG, Fehraltorf, Switzerland). The aim of this study was to determine a correlation between subjectively described complaints and the results of an objective examination of the spine using the Spinal Mouse® device. Information about the participants is given in the first part of the article. All the participants were examined with the Spinal Mouse® device in several body positions. Further, the Matthiass test was performed to evaluate neuromuscular stabilization of the axial skeleton in static conditions. Musculoskeletal pain occurred more often in students who had a higher range of motion (ROM) and had worse static stabilization of spine. Other assessed factors or measured parameters did not have any influence on musculoskeletal pain. Some of the parameters measured with the Spinal Mouse® device showed a correlation with the prevalence of musculoskeletal.

• MeSH
• bolest krku MeSH
• bolesti zad MeSH
• design vybavení MeSH
• diagnostické zobrazování přístrojové vybavení   MeSH
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• muskuloskeletální nemoci diagnostické zobrazování   epidemiologie   patologie   MeSH
• nemoci z povolání diagnostické zobrazování   epidemiologie   patologie   MeSH
• počítačové zpracování obrazu MeSH
• prevalence MeSH
• reprodukovatelnost výsledků MeSH
• rozsah kloubních pohybů fyziologie   MeSH
• studenti stomatologie statistika a číselné údaje   MeSH
• vyšetření u lůžka MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH

Musculoskeletal disorders (MSDs) frequently occur among dentists and dental students. The first aim of this study was to gather and analyze information about the prevalence of symptoms of MSDs. The second aim was to determine a correlation between subjectively described complaints and the results of an objective examination of the spine using the Spinal Mouse® device; that part is described in the second section of this article. Dental students of the first, third and fifth years at Charles University, Faculty of Medicine in Hradec Králové were included. The participants filled out a questionnaire regarding the prevalence of symptoms of MSDs and the potential risk factors. From a total of 182 students that participated in this study, 71 reported musculoskeletal pain (prevalence 39%). The musculoskeletal pain was more frequent among women. During the studies its prevalence increased. The most frequent areas of pain were neck, lower back and upper back. The results indicate that musculoskeletal pain often begins and continues to develop throughout the dentistry studies.

• MeSH
• lidé MeSH
• mladý dospělý MeSH
• muskuloskeletální nemoci epidemiologie   MeSH
• nemoci z povolání epidemiologie   MeSH
• prevalence MeSH
• průzkumy a dotazníky MeSH
• rizikové faktory MeSH
• sexuální faktory MeSH
• studenti stomatologie * MeSH
• Check Tag
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH