Q55451324
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Východiská: Vysoké hladiny prostaglandínov zistené v mnohých neoplastických tkanivách, hlavne u rakoviny hrubého čreva a prsníka, poukazujú na úlohu cyklooxygenázy v procese karcinogenézy. Materiál a metódy: Cieľom tejto štúdie bolo analyzovať chemopreventívny potenciál samostatne aplikovaného nesteroidného antiflogistika indometacínu a jeho kombinácie s pineálnym hormónom melatonínom v mamárnej karcinogenéze samíc potkanov indukovanej pomocou N-metyl-N-nitrozourey. Indometacín bol podávaný 3-krát a melatonín 4-krát v týždni, obe látky v koncentrácii 20 ?g/ml pitnej vody. Chemoprevencia začala približne 2 týždne pred aplikáciou karcinogénu a trvala do ukončenia experimentu ďalších 25 týždňov. Výsledky: Indometacín aplikovaný samostatne, ale aj v kombinácii s melatonínom stimuloval rast mamárnych tumorov, čo sa prejavilo signifikantným nárastom priemerného objemu nádorov o 126 %, resp. 104 % voči kontrolnej skupine. Samostatne podaný indometacín zvýšil incidenciu nádorov o 21,5 % (rovnako aj v kombinácii s melatonínom) a skrátil latenciu nádorov o 17 dní voči kontrole. Samotný melatonín znížil signifikantne objem nádorov porovnaním s kontrolnými zvieratami. Obe látky boli zvieratami počas dlhodobej aplikácie dobre tolerované. Záver: Indometacín, prevažný inhibítor cyklooxygenázy-1, prejavil signifikantné neoplastické účinky v prevencii N-metyl-N-nitrozoureou indukovanej mamárnej karcinogenézy u potkanov. Toto zistenie je v ostrom protiklade s našim predchádzajúcim experimentom, v ktorom sme mamárnu karcinogenézu u potkanov indukovali 7,12-dimetylbenzantracénom, pričom indometacín v tomto prípade preukázal veľmi výrazné chemopreventívne účinky.
Background: High levels of prostaglandins found in many neoplastic tissues, especially in colon cancer and breast cancer, suggest a role of cyclooxygenase in the process of carcinogenesis. Material and methods: The aim of this study was to analyse the chemopreventive potential of non-steroidal inflammatory drug indomethacin and its combination with pineal hormone melatonin in rat mammary carcinogenesis induced by N-methyl-N-nitrosourea. Indomethacin was administered 3 times a week and melatonin 4 times a week, both substances in a concentration of 20 ?g/ml of drinking water. Chemoprevention began approximately 2 weeks before carcinogen administration and lasted until the end of the experiment 25 weeks later. Results: Indomethacin administered alone and in combination with melatonin stimulated the growth of mammary tumors. We found a significant increase in the average tumor volume caused by indomethacin alone by 126%, and in combination with melatonin by 104% compared to the control group. Indomethacin administered alone increased the incidence of tumors by 21.5% (also in combination with melatonin) and reduced the tumor latency by 17 days compared to controls. Melatonin alone significantly reduced tumor volume in comparison with control animals. During the long-term administration, both substances were well tolerated by animals. Conclusion: Indomethacin, a predominant cyclooxygenase inhibitor-1, showed significant neoplastic effects in the prevention of N-methyl-N-nitrosourea induced rat mammary carcinogenesis. This finding is in strong contrast to our previous experiment, where indomethacin in 7,12-dimethylbenz(a)anthracene-induced rat mammary carcinogenesis revealed marked antineoplastic effects. Key words: rats – mammary carcinogenesis – chemoprevention – indomethacin – melatonin Submitted: 23. 2. 2012 Accepted: 30. 4. 2012
- MeSH
- antikarcinogenní látky terapeutické užití MeSH
- chemoprofylaxe MeSH
- experimentální nádory mléčných žláz * chemicky indukované prevence a kontrola MeSH
- financování organizované MeSH
- indomethacin * terapeutické užití MeSH
- melatonin terapeutické užití MeSH
- methylnitrosomočovina * MeSH
- potkani Sprague-Dawley * MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví * MeSH
- zvířata MeSH
The objective of the study was to determine some Cardiovascular Disease (CVD) risk factors in relation to cigarette smoking in 174 Roma children and adolescents (88 males and 86 females) and 131 non-Roma probands (males and females) aged 7-18 in central Slovakia. In this biethnic study, 26.4% of the Roma children and adolescents (more than twice contrary to the control group) were smokers. Among the studied ethnicities, the majority of smokers was Roma (79.3%, 46 subjects). Smoking Roma have higher means of TG, Lp(a) and WHR compared with non-smoking non-Roma. The most frequent CVD risk predictors of smoking Roma probands was low serum levels HDL-C, apo A (the Fisher test confirmed a significant relationship between cigarette smoking and HDL-C, apo A; p < 0.01). The results of the research should help to develop an effective preventative health education programs focused on Roma education (who live in a higher-risk environment compared to the majority population) in order to stem the spread of CVD as well as morbidity and mortality in this ethnic group living in Slovakia.
- MeSH
- cholesterol krev MeSH
- dítě MeSH
- financování organizované MeSH
- index tělesné hmotnosti MeSH
- kardiovaskulární nemoci epidemiologie etnologie krev MeSH
- komorbidita MeSH
- kouření epidemiologie etnologie krev MeSH
- krevní tlak MeSH
- lidé MeSH
- lipoproteiny krev MeSH
- mladiství MeSH
- poměr pasu a boků MeSH
- prevalence MeSH
- rizikové faktory MeSH
- Romové statistika a číselné údaje MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Geografické názvy
- Slovenská republika MeSH
The objective of the study was to determine some Cardiovascular Disease (CVD) risk factors in 174 Roma children and adolescents (88 males and 86 females) aged 7-18 in 3 Central Slovakian cities (44 from Zilina, 39 from Bansk, Bystrica and 91 from Rimavská Sobota). Venous blood samples were drawn in the morning, after a 12 hour overnight fast for biochemical analysis. Total cholesterol (TC) and triglycerides (TG) were determined enzymatically. HDL-cholesterol (HDL-C) after selective precipitation lipoproteins containing apolipoprotein B and LDL-cholesterol (LDL-C) was calculated by the Friedewald Formula. Serum levels of apolipoproteins (apo A, apo B) were analyzed immunochemically. Concentration of lipoprotein a [Lp(a)] was analyzed by immunonephelometric method (Beckman-Coulter System). Anthropometric measurements, including weight, height, waist and hip circumference were used to calculate the sum of the body mass index (BMI) and waist to hip ratio (WHR). Measured blood pressure (BP) was used to classify for hypertension. Significant differences were determined in serum levels of LDL-C (p < 0.05; by Tukey HSD test multiple comparison more significant difference was determined between Zilina and Rimavská Sobota p < 0.046), TG (p = 0.008), apo A (p < 0.001), Lp(a) (p = 0.042), WHR (p < 0.001), BMI (p < 0.001), sBP (p < 0.001) and dBP (p = 0.012) in Roma individuals of all locality groups. The Roma population from Rimavsk, Sobota had (in comparison to the examined populations) statistically higher values of TC, TG, LDL-C, lower HDL-C. The population showed significant relation of TG and stress at home (p = 0.03) and at school (p = 0.01), HDL-C and cigarette smoking (p = 0.004), apo A and cigarette smoking (p = 0.02) and socioeconomic status (p = 0.006), WHR and cigarette smoking (p = 0.02). Risk values of WHR, apo B and Lp(a) were mostly determined in Zilina's population (WHR significantly connected with family history CVD p = 0.03, cigarette smoking p = 0.02 and leisure time physical activity p < 0.001) and BMI, apo A and BP in Banská Bystrica. WHR was positively correlated to BP and negatively to HDL-C and TG only in Roma participants from Rimavská Sobota. BMI was positively correlated to systolic BP in populations from Banská Bystrica and Rimavská Sobota. The results of the study should improve the paediatric health treatment and prevention of CVD risk predictors for Roma from different cities.
- MeSH
- adipozita MeSH
- charakteristiky bydlení MeSH
- dítě MeSH
- financování organizované MeSH
- kardiovaskulární nemoci etnologie prevence a kontrola MeSH
- lidé MeSH
- lipidy krev MeSH
- mladiství MeSH
- rizikové faktory MeSH
- Romové statistika a číselné údaje MeSH
- životní styl MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- multicentrická studie MeSH
- Geografické názvy
- Slovenská republika MeSH
- MeSH
- antiflogistika nesteroidní aplikace a dávkování MeSH
- cholesterol krev metabolismus MeSH
- glukosa metabolismus MeSH
- krysa rodu rattus MeSH
- tma MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
