The design of favorable mechanical properties and suitable surface modifications of hydrogels in order to stimulate specific cell response is a great challenge. N-(2-Hydroxypropyl) methacryl-amide (HPMA) was utilized to form macroporous cryogel scaffolds for stem cell applications. Furthermore, one group of scaffolds was enhanced by copolymerization of HPMA with methacryloyl-GGGRGDS-OH peptide in an effort to integrate biomimetic adhesion sites. The cryogels were characterized by stiffness and equilibrium swelling measurements as well as by scanning electron microscopy. Cell culture experiments were performed with human adipose-derived stem cells and substrates were found completely non-toxic. Moreover, RGDS-enriched cryogels supported cell attachment, spreading and proliferation, so they can be considered suitable for designed aims.

• MeSH
• akrylamidy * MeSH
• biokompatibilní materiály MeSH
• biomimetika * MeSH
• buněčná adheze MeSH
• kmenové buňky * MeSH
• kryogely MeSH
• kultivované buňky MeSH
• lidé MeSH
• poréznost MeSH
• proliferace buněk MeSH
• pružnost MeSH
• tkáňové podpůrné struktury MeSH
• tukové buňky MeSH
• voda chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH