The use of microfluidic sperm sorting (MFSS) systems in infertility treatment is increasing due to their practicality and ease of use. While often presented as highly effective, their efficacy in patients with varying sperm analysis results remains uncertain. In this study, we evaluated the effectiveness of MFSS compared with the swim-up (SU) technique in terms of oxygen radical levels and spermiogram parameters. Samples from each patient were processed using both methods, followed by assessments of sperm concentration, motility, morphology, DNA integrity, acrosomal status, and mitochondrial membrane potential. Participants were selected based on sperm analysis and categorized as normozoospermic (n = 40) or non-normozoospermic (n = 28). An analysis of separation techniques revealed no significant differences, except for a lower percentage of DNA-fragmented sperm in the MFSS group compared with SU within the non-normozoospermic cohort (SU: 10.0% vs. MFSS: 5.69%, p = 0.027). No differences were observed between SU and MFSS in normozoospermic men. The MFSS method is a simple technique, frequently used in laboratories, that yields good results but does not offer a substantial advantage over SU. The primary benefit of MFSS appears to be a significant reduction in the proportion of sperm with DNA fragmentation compared with SU in patients with abnormal sperm analysis results.

• MeSH
• analýza spermatu metody   MeSH
• dospělí MeSH
• fragmentace DNA MeSH
• intracytoplazmatické injekce spermie * metody   MeSH
• lidé MeSH
• membránový potenciál mitochondrií MeSH
• mikrofluidika * metody   MeSH
• motilita spermií * MeSH
• mužská infertilita terapie   MeSH
• separace buněk * metody   MeSH
• spermie * cytologie   metabolismus   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH

Obesity can adversely affect human health, including fertility. While obesity can disturb the hormonal profile of the female organism and is associated with fertility loss, little is known about what effect male obesity has on fertility. The present study analysed sperm samples of 153 donors. The men were selected from couples attending an infertility clinic, who had tried for 12 months or more to achieve pregnancy without success. The age of the men under investigation was recorded, and their body mass index (BMI) was calculated. All semen samples were assessed for volume, concentration, motility and morphology. Sperm chromatin integrity was measured by sperm chromatin structure assay. Quality of sperm chromatin condensation was assessed by toluidine blue, aniline blue and chromomycin A3 staining. We can conclude that the impact of elevated BMI on the parameters investigated (basic semen parameters, chromatin integrity and chromatin condensation) was not proven in this study. On the other hand, ejaculate quality appeared to be affected by ageing. The impact was reflected by chromatin integrity, which is a factor that can substantially affect fertility in men, rather than by basic sperm parameters.

• MeSH
• analýza spermatu MeSH
• chromatin genetika   MeSH
• dospělí MeSH
• fragmentace DNA MeSH
• index tělesné hmotnosti MeSH
• lidé středního věku MeSH
• lidé MeSH
• motilita spermií MeSH
• mužská infertilita etiologie   MeSH
• obezita komplikace   MeSH
• počet spermií MeSH
• sperma MeSH
• spermie cytologie   MeSH
• stárnutí MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Cíl studie: Vyhodnocením základních spermiologických parametrů podle standardů WHO u potenciálních dárců spermatu přispět k současné diskusi o mužském faktoru neplodnosti. Typ studie: Retrospektivní demografická studie. Název a sídlo pracoviště: Gyn.-por. klinika LF UP a FN Olomouc, Ústav biologie LF UP Olomouc. Metodika: V období leden 2000 – duben 2004 se dostavilo ke vstupnímu vyšetření celkem 108 potenciálních dárců spermií ve věku 18–35 let. Spermiologické vyšetření čerstvého semene získaného masturbací bylo provedeno podle standardů WHO. Vyhodnocovanými parametry byly objem ejakulátu, koncentrace spermií a celková motilita. Výsledky: Normospermie byla zjištěna jen u 46 mužů (42,6 %). U tří mužů (2,8 %) byla prokázána azoospermie. Nejčastějším patologickým nálezem byla astenospermie, která byla zjištěna u 49 (46,6 %) mužů. Závěr: Na základě rozboru získaných dat můžeme usuzovat na zřejmý pokles kvality spermiologických parametrů mladé české populace. Podle našeho názoru by provedení multicentrické analýzy se zaměřením na hodnocení plodnosti českých mužů bylo vysoce žádoucí.

Objective: To contribute to the contemporary discussion on the male factor infertility through the evaluation of the basic spermatological parameters in men recruiting for semen donation. Design: A retrospective demographic study. Setting: Dept. of Obstetrics and Gynaecology, Dept. of Biology, Faculty of Medicine, Palacký University, Olomouc. Methods: A total of 108 men aged 18 to 35 years came to the preliminary examination for the potential semen donors between January 2000 and April 2004. Spermatological analysis of the fresh ejaculates obtained by masturbation was performed according to WHO guidelines. The following parameters were evaluated: the volume of ejaculate, sperm concentration and total percent motility. Results: Normospermia was found in only 46 men (42.6%). Three males (2.8%) were azoospermic. The most frequent problem was asthenospermia, which was documented in 49 (46.6%) men. Conclusion: The analysis of the obtained data may suggest a marked semen quality decline in the Czech male population. In our opinion, further multicentric epidemiological studies concerning fertility potential in Czech male population seem highly needed.

• MeSH
• dospělí MeSH
• finanční podpora výzkumu jako téma MeSH
• lidé MeSH
• motilita spermií MeSH
• mužská infertilita diagnóza   patologie   MeSH
• počet spermií MeSH
• reprodukční lékařství MeSH
• retrospektivní studie MeSH
• spermabanky MeSH
• žijící dárci MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH

The factors affecting the inter-individual differences in sperm freezability is a major line of research in spermatology. Poor sperm freezability is mainly characterised by a low sperm velocity, which in turn is associated with low fertility rates in most animal species. Studies concerning the implications of sperm morphometry on freezability are quite limited, and most of them are based on sperm head size regardless of the structural parts of the flagellum, which provides sperm motility. Here, for the first time, we determined the volumes of the flagellum structures in fresh epididymal red deer spermatozoa using a stereological method under phase contrast microscopy. Sperm samples from thirty-three stags were frozen and classified as good freezers (GF) or bad freezers (BF) at two hours post-thawing using three sperm kinetic parameters which are strongly correlated with fertility in this species. Fourteen stags were clearly identified as GF, whereas nineteen were BF. No significant difference in sperm head size between the two groups was found. On the contrary, the GF exhibited a lower principal piece volume than the BF (6.13 µm3 vs 6.61 µm3, respectively, p = 0.006). The volume of the flagellum structures showed a strong negative relationship with post-thawing sperm velocity. For instance, the volume of the sperm principal piece was negatively correlated with sperm velocity at two hours post-thawing (r = -0.60; p<0.001). Our results clearly show that a higher volume of the sperm principal piece results in poor freezability, and highlights the key role of flagellum size in sperm cryopreservation success.

• MeSH
• analýza hlavních komponent MeSH
• bičík spermie fyziologie   MeSH
• epididymis cytologie   MeSH
• hlavička spermie fyziologie   MeSH
• kinetika MeSH
• kryoprezervace metody   MeSH
• mikroskopie fázově kontrastní MeSH
• motilita spermií fyziologie   MeSH
• organely fyziologie   MeSH
• shluková analýza MeSH
• spermie klasifikace   fyziologie   MeSH
• uchování spermatu metody   MeSH
• vysoká zvěř MeSH
• zmrazování * MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Sexually mature males (BW = 1600 ± 150 g and TL = 235 ± 30 mm) of northern pike (Esox lucius L.) were randomly selected from a pond to record changes in their sperm quality parameters (spermatozoa morphology, sperm volume, density, and motility parameters) during the spawning season. The morphological and motility parameters changed significantly during the reproductive season with following trends. Only, head width was not changed during the spawning season. The longest spermatozoa and its flagellar length were found at the middle of spawning period (TL = 38.24 ± 0.37 μm and 35.14 ± 0.26 μm) and shortest at the beginning of spawning period (TL = 34.81 ± 0.29 μm and 32.53 ± 0.18 μm). Other morphological characters were always the lowest at the beginning of spawning period. Sperm volume was changed from 0.33 ± 0.3 ml in February, 0.43 ± 0.2 ml in March to 0.24 ± 0.1 ml in April, and density from 16.2 ± 0.2 × 109 spermatozoa ml-1 in February, 19.4 ± 0.2 × 109 spermatozoa ml-1 in March to 4.8 ± 0.2 × 109 spermatozoa ml-1 in April. Same sperm velocity was observed in all spawning terms at 10 and 20 s after activation. Higher velocity was found at 30 and 40 s after activation in sperm collected at the middle and the end of spawning period. Significantly, higher percentage of motile sperm was observed at 20, 30, and 40 s after activation in sperm sampled at the end of spawning period. This study supports the hypothesis that longer spermatozoa swim faster.

• MeSH
• analýza spermatu veterinární   MeSH
• Esocidae anatomie a histologie   růst a vývoj   fyziologie   MeSH
• motilita spermií * MeSH
• roční období MeSH
• rozmnožování MeSH
• sexuální chování zvířat * MeSH
• spermatogeneze * MeSH
• spermie fyziologie   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• dospělí MeSH
• fertilizace in vitro MeSH
• kadmium krev   metabolismus   škodlivé účinky   MeSH
• kouření MeSH
• lidé MeSH
• motilita spermií MeSH
• mužská infertilita diagnóza   etiologie   MeSH
• oligospermie MeSH
• spermatogeneze MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• přehledy MeSH
• srovnávací studie MeSH

The effects of heavy metals (Cd, Cr and Cd+Cr) on the motility parameters and oxidative stress of sterlet (Acipenser ruthenus) sperm were investigated in vitro. Sturgeon sperm were exposed for 2h to heavy metals at environmental related concentrations (0.1mgL(-1) Cr, 0.001mgL(-1) Cd, 0.1mgL(-1) Cr+0.001mgL(-1) Cd) and higher concentrations (5.0mgL(-1) Cr, 0.05mgL(-1) Cd, 5.0mgL(-1) Cr+0.05mgL(-1) Cd). Results revealed that environmental concentrations of heavy metals had no significant influence on motility parameters and antioxidant responses indices in sturgeon sperm, except for LPO level and SOD activity. But higher concentrations of these metals induced oxidative tress in sturgeon sperm in vitro, associated with sperm motility parameters inhibition. Our results suggest that using of sperm in vitro assays may provide a novel and efficiently means for evaluating the effects of residual heavy metals in aquatic environment on sturgeon.

• MeSH
• analýza hlavních komponent MeSH
• antioxidancia metabolismus   MeSH
• časové faktory MeSH
• chemické látky znečišťující vodu toxicita   MeSH
• monitorování životního prostředí metody   MeSH
• motilita spermií účinky léků   MeSH
• oxidační stres účinky léků   MeSH
• ryby MeSH
• spermie cytologie   účinky léků   enzymologie   metabolismus   MeSH
• těžké kovy toxicita   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• životní prostředí MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

In this study, we examined different computer-assisted sperm analysis (CASA) systems (CRISMAS, Hobson Sperm Tracker, and Image J CASA) on the exact same video recordings to evaluate the differences in sperm motility parameters related to the specific CASA used. To cover a wide range of sperm motility parameters, we chose 12-second video recordings at 25 and 50 Hz frame rates after sperm motility activation using three taxonomically distinct fish species (sterlet: Acipenser ruthenus L.; common carp: Cyprinus carpio L.; and rainbow trout: Oncorhynchus mykiss Walbaum) that are characterized by essential differences in sperm behavior during motility. Systematically higher values of velocity and beat cross frequency (BCF) were observed in video recordings obtained at 50 Hz frame frequency compared with 25 Hz for all three systems. Motility parameters were affected by the CASA and species used for analyses. Image J and CRISMAS calculated higher curvilinear velocity (VCL) values for rainbow trout and common carp at 25 Hz frequency compared with the Hobson Sperm Tracker, whereas at 50 Hz, a significant difference was observed only for rainbow trout sperm recordings. No significant difference was observed between the CASA systems for sterlet sperm motility at 25 and 50 Hz. Additional analysis of 1-second segments taken at three time points (1, 6, and 12 seconds of the recording) revealed a dramatic decrease in common carp and rainbow trout sperm speed. The motility parameters of sterlet spermatozoa did not change significantly during the 12-second motility period and should be considered as a suitable model for longer motility analyses. Our results indicated that the CASA used can affect motility results even when the same motility recordings are used. These results could be critically altered by the recording quality, time of analysis, and frame rate of camera, and could result in erroneous conclusions.

• MeSH
• analýza spermatu metody   veterinární   MeSH
• motilita spermií * MeSH
• počítačové zpracování obrazu přístrojové vybavení   MeSH
• ryby * MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH

Cíl: Analýza spermiogramů onkologických pacientů, posouzení vztahu patologie spermatu s onkologickou diagnózou, stanovení počtu zemřelých a využití zamrazeného spermatu za 11 let provozu onkologické spermabanky. Typ studie: Retrospektivní klinická studie. Název a sídlo pracoviště: Gynekologicko-porodnická klinika LF MU Brno. Metodika: V letech 1995–2005 bylo odesláno na zamražení spermatu před terapií maligního nádoru chemoterapií, aktinoterapií nebo orchidektomií celkem 521 mužů ve věku od 14 do 64 let (26,0±6,7 roku). Hodnocení spermiogramu bylo provedeno podle manuálu Světové zdravotnické organizace. Kryonkonzervace spermatu s kryoprotektivním médiem byla provedena na přístroji Planer Kryo F10 standardní křivkou ochlazování. Data byla hodnocena základní popisnou statistikou, vztahy mezi parametry spermiogramu a diagnózou byly testovány Kruskal-Wallis ANOVA. Výsledky: Zhoubný nádor varlete byl diagnostikován u 45,7 % pacientů, maligní onemocnění mízní a krvetvorné tkáně v 32,0 %, z toho Hodgkinův lymfom v 16,5 %, non-Hodgkinův lymfom v 7,1 % a leukémie v 8,4 %. K dalším diagnózám patřila zhoubná onemocnění kosti, pojiva, gastrointestinálního traktu, ledvin a retroperitonea. Koncentraci spermií ? 20 mil/ml mělo 45,9 % mužů. Těžkou oligospermii ? 1 mil/ml mělo 23,2 % mužů a azoospermii 10,0 % pacientů. Těžká astenospermie ?10 % byla prokázána u 60,8 % mužů. Ve skupině 30 chlapců ve věku 14 až 16 let byly spermie získány v 76,7 % případů, z toho v 46,7 % byla koncentrace nižší než 1 mil/ml a v 26,1 % pohyb nižší než 1 %. Zamrazené sperma bylo dosud využito u 30 pacientů (5,8 %), z toho u 22 v našem centru. Ve všech případech byla použita technika ICSI, bylo dosaženo 7 klinických těhotenství a 5 porodů. Koncentrace spermií byla signifikantně nižší u mužů se zhoubným nádorem varlete (p = 0,002), rozdíly motility nebyly signifikantní. V souboru pacientů bylo evidováno celkem 62 úmrtí (11,9 %). Specifickou otázkou je žádost o využití spermatu zemřelých, velmi důležitá je spolupráce s etickou komisí a právním oddělením. Závěr: U onkologických pacientů byly prokázány ve vysokém procentu těžké patologie spermatu. Nejnižší koncentrace spermií jsou u mužů se zhoubným nádorem varlete. Kryokonzervované spermie jsou schopny s využitím metod asistované reprodukce dosáhnout těhotenství, zmrazené sperma však využije pro léčbu neplodnosti jen malé procento pacientů. Celkem zemřelo 11,9 % mužů odeslaných k zamražení spermatu. Program onkologické spermabanky vyžaduje úzkou spolupráci centra asistované reprodukce s onkologickým pracovištěm.

Introduction: The aim of this study was to analyze possible relation of sperm pathology and diagnosis of malignant disease and present our experience of sperm banking programme for cancer patients. Design: Retrospective clinical study. Setting: Department of Obstetrics and Gynecology, Masaryk University, Brno. Material and methods: 521 patients (age 26.0±6.7 years) were referred to our unit by oncology specialists for semen cryopreservation before cancer treatment during 1995–2005. Sperm samples obtained by masturbation were analysed according to guidelines of WHO. Cryoprotective medium and Planer Kryo F 10 were used for standard cryopreservation. National cancer register was used to analyze patients survival. Basic statistics was used for data description and relations among sperm parameters and diagnosis were tested by Kruskal-Wallis ANOVA. Standard intracytoplasmic sperm injection (ICSI) using frozen spermatozoa was used for infertility treatment following malignant disease recovery. Results: Testicular cancer was diagnosed in 45.7% patients, malignant disease of lymphatic and haemopoetic tissue in 32.0% cases – out of them 16.5% Hodgkinęs lymphoma. Sperm concentration ? 20 mil/ml had 45.9% men. Severe oligospermia ? 1 mil/ml was found in 23.2% men and azoospermia in 10.0% patients. Severe asthenospermia ?10% was in 60,8% men. Sperms were retrieved in 76.7% of 30 boys 14 to 16 years old, in 46.7% was concentration lower than 1 mil/ml and in 26.1% sperm motility lower than 1%. Sperm concentration was significantly lower in men with testicular cancer (p = 0.002), differences in sperm motility were insignificant. Cryopreserved samples were used in 30 patients (5.8%), 22 of them in our centre. Intracytoplasmatic sperm injection was used in all cases, 7 clinical pregnancies (31.8%) and 5 deliveries were achieved. In the group of all patients died 62 men (11.9%). Conclusion: Cancer patients have high frequency of severe sperm pathology. The lowest sperm concentration was in men with testicular cancer. Only minority of patients return for fertility treatment. Cryopreserved spermatozoa of cancer patients are able to achieve pregnancy by assisted reproduction techniques.

• MeSH
• fertilizace in vitro metody   trendy   využití   MeSH
• financování organizované MeSH
• kryoprezervace metody   využití   MeSH
• lidé MeSH
• nádory mužských pohlavních orgánů komplikace   terapie   MeSH
• nádory terapie   MeSH
• protinádorové látky škodlivé účinky   MeSH
• sperma MeSH
• spermabanky metody   trendy   využití   MeSH
• těhotenství MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• těhotenství MeSH
• ženské pohlaví MeSH

OBJECTIVE: The aim of this study was to analyse the long-term radiation effects on human sperm. METHODS: In total, 104 samples of male donors from 2 regions of Ukraine were tested. Group 1 consisted of 32 donors from the Ivano-Frankivsk region, group 2 included 72 volunteers from the Zhytomyr region. The average age of donors in both groups was 35 ± 6 years (range 24-49). To assess the level of apoptosis, membrane mitochondrial potential, concentration of reactive oxygen species, and ploidy of sperm, flow cytometry was performed. RESULTS: The individual equivalent dose of group 1 was < 0.4 mSv and of group 2 ≥ 0.4 mSv. Live spermatozoa with signs of apoptosis were significantly higher (p < 0.05) in group 2 in comparison to group 1 (15.6% and 11.2%, respectively). Spermatozoa without violating integrity were 73.2% in group 1 and approximately 16% higher than the indices of group 2. The percentage of dead necrotic spermatozoa was twice as high in men with a predicted equivalent dose of ≥ 0.4 mSv than in comparison group. A higher percentage of spermatozoa with low mitochondrial membrane potential, di- and tetraploid was found in group 2. CONCLUSIONS: An equivalent individual dose of ≥ 0.4 mSv can cause a decrease in mitochondrial potential, an increase in the production of spermatozoa with pathological ploidy, as well as to provoke increasing apoptosis in cells.

• MeSH
• černobylská havárie * MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• průtoková cytometrie MeSH
• reaktivní formy kyslíku farmakologie   MeSH
• sperma MeSH
• spermie patologie   účinky záření   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH