Metal-organic frameworks (MOFs) have been widely used as porous nanomaterials for different applications ranging from industrial to biomedicals. An unpredictable one-pot method is introduced to synthesize NH2-MIL-53 assisted by high-gravity in a greener media for the first time. Then, porphyrins were deployed to adorn the surface of MOF to increase the sensitivity of the prepared nanocomposite to the genetic materials and in-situ cellular protein structures. The hydrogen bond formation between genetic domains and the porphyrin' nitrogen as well as the surface hydroxyl groups is equally probable and could be considered a milestone in chemical physics and physical chemistry for biomedical applications. In this context, the role of incorporating different forms of porphyrins, their relationship with the final surface morphology, and their drug/gene loading efficiency were investigated to provide a predictable pattern in regard to the previous works. The conceptual phenomenon was optimized to increase the interactions between the biomolecules and the substrate by reaching the limit of detection to 10 pM for the Anti-cas9 protein, 20 pM for the single-stranded DNA (ssDNA), below 10 pM for the single guide RNA (sgRNA) and also around 10 nM for recombinant SARS-CoV-2 spike antigen. Also, the MTT assay showed acceptable relative cell viability of more than 85% in most cases, even by increasing the dose of the prepared nanostructures.
- MeSH
- buňky Hep G2 MeSH
- buňky PC12 MeSH
- COVID-19 diagnóza MeSH
- CRISPR-Cas systémy MeSH
- dusík chemie MeSH
- guide RNA, Kinetoplastida MeSH
- HEK293 buňky MeSH
- HeLa buňky MeSH
- jednovláknová DNA MeSH
- krysa rodu rattus MeSH
- lidé MeSH
- limita detekce MeSH
- nanokompozity MeSH
- nanostruktury MeSH
- porézní koordinační polymery chemie MeSH
- poréznost MeSH
- porfyriny chemie MeSH
- povrchové vlastnosti MeSH
- RNA virová metabolismus MeSH
- SARS-CoV-2 MeSH
- senzitivita a specificita MeSH
- testování na COVID-19 MeSH
- vodíková vazba MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
