- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
The diseases associated with tobacco smoking affect miRNAs and small single-stranded non-coding RNAs. However, there are no data on urinal miRNAs in healthy smokers. We searched for the possible effect of smoking and smoking cessation on miRNA urine expression. For screening, Affymetrix miRNA 4.0 arrays were used in 33 urine samples obtained from six never smokers and from current smokers in three time-points before smoking cessation (n = 10), after short time abstinence (3-8 weeks), and after long-term abstinence (1 year). For validation, a quantitative (q) polymerase chain reaction (PCR) method was used in 93 urine samples obtained from 18 never smokers and 25 current smokers in three time-points before smoking cessation, after short time abstinence (3-8 weeks), and after long-term abstinence (1 year). In screening analysis, 5 miRNAs (hsa-miR-3620-5p, hsa-miR-3613-5p, hsa-miR-3921, hsa-miR-5094, and hsa-miR-337-3p) were dysregulated in current vs. never smokers after multiple testing corrections. Smoking cessation was accompanied by miRNA dysregulation that did not reach a significant level after a multiple testing correction. In validation analysis, three miRNAs correlated with cotinine, but they were affected neither after smoking cessation nor between current and never smokers. Our whole-genome screening of 2.578 miRNAs and validation suggest that tobacco smoking has no or only a small effect on urinal miRNAs.
- Publikační typ
- časopisecké články MeSH
- MeSH
- lékařská onkologie * MeSH
- pneumologie * MeSH
- Publikační typ
- biografie MeSH
- nekrology MeSH
- O autorovi
- Kolek, Vítězslav, 1953-2020 Autorita
- O autorovi
- Kolek, Vítězslav, 1953-2020 Autorita
- O autorovi
- Kolek, Vítězslav, 1953-2020 Autorita
- MeSH
- antituberkulotika MeSH
- diferenciální diagnóza MeSH
- nádory kůže MeSH
- senioři MeSH
- tuberkulóza kůže * diagnostické zobrazování farmakoterapie MeSH
- výsledek terapie MeSH
- Check Tag
- mužské pohlaví MeSH
- senioři MeSH
- Publikační typ
- kazuistiky MeSH
