Nuclear myosin I (NMI) is a single-headed member of myosin superfamily localized in the cell nucleus which participates along with nuclear actin in transcription and chromatin remodeling. We demonstrate that NMI is present in cell nuclei of all mouse tissues examined except for cells in terminal stages of spermiogenesis. Quantitative PCR and western blots demonstrate that the expression of NMI in tissues varies with the highest levels in the lungs. The expression of NMI is lower in serum-starved cells and it increases after serum stimulation. The lifespan of NMI is longer than 16 h as determined by cycloheximide translation block. A homologous protein is expressed in human, chicken, Xenopus, and zebrafish as shown by RACE analysis. The analysis of genomic sequences indicates that almost identical homologous NMI genes are expressed in mammals, and similar NMI genes in vertebrates.

• MeSH
• buněčné jádro metabolismus   MeSH
• buněčné linie MeSH
• exprese genu MeSH
• financování organizované MeSH
• fylogeneze MeSH
• genetická transkripce MeSH
• konzervovaná sekvence MeSH
• lidé MeSH
• myosin typu I genetika   chemie   izolace a purifikace   metabolismus   MeSH
• myši MeSH
• obratlovci genetika   metabolismus   MeSH
• sekvence aminokyselin MeSH
• sekvenční homologie nukleových kyselin MeSH
• sérum chemie   MeSH
• techniky amplifikace nukleových kyselin MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH