The aim of this work was to compare in vitro nitric oxide (NO) production by rat, bovine and porcine macrophages. NO production was induced by lipopolysaccharide (LPS) or by phorbol 12-myristate 13-acetate (PMA) with ionomycin or recombinant interferon gamma (rIFN-gamma) and was assessed by Griess reaction. NO synthase type II (NOS II) expression was quantified by immunocytochemistry, Western blot and real-time polymerase chain reaction (RT-PCR). There were differences in NO production by pulmonary alveolar macrophages (PAM) in all species tested. The largest amounts of NO were produced by rat PAM. Less NO was produced by bovine PAM. Moreover, PAM in rats and cows differed in their abilities to respond to various stimulators. Neither porcine PAM nor Kupffer cells produced NO. Stimulation of porcine PAM with alternative concentrations of LPS did not lead to inducing NO production. Stimulation of porcine PAM with rIFN-gamma together with LPS led to a significant increase in the expression of NOS II mRNA, albeit without detectable NO production or NOS II expression on the protein level.

• MeSH
• alveolární makrofágy metabolismus   patologie   MeSH
• biotest MeSH
• ethylendiaminy MeSH
• financování organizované MeSH
• imunohistochemie MeSH
• interferon gama toxicita   MeSH
• ionomycin toxicita   MeSH
• krysa rodu rattus MeSH
• kultivované buňky MeSH
• lipopolysacharidy toxicita   MeSH
• messenger RNA metabolismus   MeSH
• oxid dusnatý analýza   metabolismus   MeSH
• polymerázová řetězová reakce s reverzní transkripcí MeSH
• prasata MeSH
• regulace genové exprese enzymů MeSH
• skot MeSH
• sulfanilamidy MeSH
• synthasa oxidu dusnatého, typ II genetika   metabolismus   MeSH
• tetradekanoylforbolacetát toxicita   MeSH
• western blotting MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• skot MeSH
• zvířata MeSH