A total 355 of Staphylococcus cohnii isolates from hospital environment, patients (newborns), medical staff and from non-hospital environment were tested for hemolytic activity. Ninety-one % of S. cohnii ssp. cohnii and 74.5 % S. cohnii ssp. urealyticus strains exhibited hemolysis synergistic to S. aureus ATCC 25923 strain. Crude preparations of hemolysins of both bacterial subspecies presented delta-hemolysin, but not alpha- and beta-toxin activity. Highly pure hemolysins were obtained by semipreparative SDS-PAGE or by organic solvent extraction from the freeze-dried crude preparations. Native-PAGE and 2D-PAGE showed their high heterogeneity. Molar masses of single hemolysin units estimated by the Tris-Tricine-SDS-PAGE were calculated as 3.47 kDa for S. cohnii ssp. cohnii and 3.53 kDa for S. cohnii ssp. urealyticus.

• MeSH
• dospělí MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• hemolyziny izolace a purifikace   metabolismus   MeSH
• infekce spojené se zdravotní péčí mikrobiologie   MeSH
• isoelektrická fokusace MeSH
• kůže mikrobiologie   MeSH
• lidé MeSH
• molekulová hmotnost MeSH
• novorozenec MeSH
• personál nemocniční MeSH
• stafylokokové infekce mikrobiologie   MeSH
• Staphylococcus chemie   izolace a purifikace   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• novorozenec MeSH
• Geografické názvy
• Polsko MeSH