BACKGROUND: The objective is to study the effect of after-birth oral colonization by a probiotic Escherichia coli strain in infants of allergic mothers to reduce occurrence of allergy later in life. METHODS: In a controlled clinical trial, 158 infants were randomly divided into groups of (i) 56 colonized infants of allergic mothers, (ii) 57 control infants of allergic mothers, and (iii) 45 control infants of healthy mothers. Incidence rates of bacterial pathogens in stool and levels of anti-E. coli immunoglobulins and some cytokines in serum were determined, and secretory IgA was monitored in stool filtrates and maternal milk. Clinical check-ups of infants aged 4 days, 3 and 6 months, 2, 3 and 5 years were carried out and clinical symptoms of allergy were monitored. One milliliter of the probiotic E. coli strain was administered to infants of allergic mothers at first within 48 h after birth and subsequently 3 times a week over a period of 4 weeks. Control infants of allergic and healthy mothers were monitored in these intervals as well. RESULTS: Presence of the E. coli strain was monitored in stool samples throughout the study. At the conclusion of the study, allergy symptoms were found in 14 infants of control allergic mothers, 7 infants of healthy mothers, and in 2 colonized infants of allergic mothers. Colonization affected levels of several cytokines and specific anti-E. coli antibodies. CONCLUSIONS: After birth, targeted colonization of the intestine by a probiotic E. coli strain can be an effective means of allergy prevention in infants of allergic mothers.

• MeSH
• alergie prevence a kontrola   MeSH
• cytokiny krev   MeSH
• dospělí MeSH
• Escherichia coli MeSH
• imunoglobulin E krev   MeSH
• lidé MeSH
• novorozenec MeSH
• probiotika terapeutické užití   MeSH
• střeva mikrobiologie   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• novorozenec MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• randomizované kontrolované studie MeSH

Perorální osídlení probiotickým kmenem E. coli (vakcina COLINFANT) dětí alergických matek po narození snížilo významně: 1) výskyt bakteriálních patogenů ve střevě, především druhů Klebsiella, Pseudomonas aeruginosa a Staphyloccocus epidermidis. 2) výskyt klinických příznaků alergie - u osídlené skupiny dětí alergických matek sledovaných do 3 let věku byla alergie prokázána pouze u jednoho dítěte, zatímco u kontrolních, neosídlených dětí byla alergie prokázána u 14 dětí (36%, p< 0,001). První případy se u kontrolní skupiny vyskytly již ve věku 3 měsíců. 3) Hladiny IgE byly zvýšené u 83% a 84% skupin alergických matek, 16% dětí osídlené sklupiny a 8% dětí kontrolní skupiny alergických matek. 4) Ovlivnilo hladiny některých cytokinů (IL-4,IL-5, IL-6 a IL-13). Hladinu těchto cytokinů u osídlených dětí alergických matek zůstaly podobné jako u zdravých matek, zatímco u kontrolních dětí alergických matek jejich hladiny mezi 2-3 rokem stoupaly. Ve studii bylo prokázáno, že osídlení probiotickýcm kmenem E. coli po narození je účinným preventivním opatřením u dětí alergických matek.

Oral colonization with a probiotic E.coli strain afer birth in infa nts of allergic mothers decrea sed significantly: 1) The numb er of bacterial pathogens in the intestine, particularly strains of Pseudo monas aeruginosa, Klebsiella and Staphyloccocus epidermidis. 2) Incid ence of clinical symptoms of allergy – from the colonized group only 1 child su ffered from alllergy, while in control infants of allergic mother s allergies appeared in l4 infants (36 %, p < 0.00l). 3) IgE levels were increased in 83 % and 84 % of allergic mothers, l6 % of infants of colonize d and 8 % of control allergic mothers. 4) Levels of some cytokines ( IL-4, IL-5, IL-6 and IL-l3) equaled those of children from healthy mothers, but increased in infants of allergic control mothers between years 2-3. Targeted colonization of the intestine after birth with a probiotic E. coli strain may be a useful preventive measure in infant s of allergic mothers.

• Klíčová slova
• Colinfant,
• MeSH
• alergie diagnóza   krev   prevence a kontrola   MeSH
• finanční podpora výzkumu jako téma MeSH
• imunita získaná od matky účinky léků   účinky záření   MeSH
• lidé MeSH
• matky MeSH
• novorozenec MeSH
• probiotika aplikace a dávkování   farmakologie   MeSH
• střevní sliznice mikrobiologie   MeSH
• vakcíny proti Escherichia coli aplikace a dávkování   farmakologie   imunologie   MeSH
• Check Tag
• lidé MeSH
• novorozenec MeSH

Allergic diseases belong to one of the most common diseases with steadily increasing incidence even among young children. There is an urgent need to identify a prognostic marker pointing to increased risk of allergy development enabling early preventive measures introduction. It has been shown that administration of selected probiotic strains or mixtures could prevent allergy development. In our study, we have tested the capacity of probiotic strain Escherichia coli O83:K24:H31 (E. coli O83) to promote dendritic cell (DC) maturation and polarisation of immune responses. Increased presence of activation marker CD83 was observed on DC stimulated by E. coli O83 and DC of newborns of allergic mothers have significantly more increased cell surface presence of CD83 in comparison to children of healthy mothers. Increased gene expression and secretion of IL-10 was detected in DC stimulated with E. coli O83 being higher in DC of newborns of healthy mothers in comparison to allergic ones. Generally, increased presence of intracellular cytokines (IL-4, IL-13, IFN-gamma, IL-17A, IL-22, IL-10) was detected in CD4+ T cells cocultured with DC of children of allergic mothers in comparison to healthy ones. E. coli O83 primed DC significantly increased IL-10 and IL-17A in CD4 T cells of newborns of healthy mothers in comparison to the levels detected in CD4 T cells cocultured with control non-stimulated DC. We can conclude E. coli O83 induces dendritic cell maturation and IL-10 production in DC. Newborns of allergic mothers have generally increased reactivity of both DC and CD4 T cells which together with decreased capacity of DC of newborns of allergic mothers to produce IL-10 could support inappropriate immune responses development after allergen encounter.

• MeSH
• aktivace lymfocytů MeSH
• alergie imunologie   MeSH
• buněčná diferenciace MeSH
• CD4-pozitivní T-lymfocyty imunologie   MeSH
• dendritické buňky mikrobiologie   fyziologie   MeSH
• dospělí MeSH
• Escherichia coli fyziologie   MeSH
• imunita získaná od matky MeSH
• interleukin-10 metabolismus   MeSH
• interleukin-17 metabolismus   MeSH
• interleukiny metabolismus   MeSH
• kokultivační techniky MeSH
• kultivované buňky MeSH
• lidé MeSH
• matky MeSH
• novorozenec MeSH
• probiotika MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• novorozenec MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Probiotics are believed to prevent or reduce allergy development but the mechanism of their beneficial effect is still poorly understood. Immune characteristics of regulatory T cells (Tregs) in peripheral blood of perinatally probiotic-supplemented children of allergic mothers (51 children), non-supplemented children of allergic mothers (42 children), and non-supplemented children of healthy mothers (28 children) were compared at the age of 6-7 years. A first dose of a probiotic Escherichia coli strain (E. coli O83:K24:H31) was administered within 2 days after the birth and then 12 times during the first months of life and children were followed longitudinally. Proportion and functional properties of Tregs were estimated by flow cytometry in relation to the children's allergy status. Proportion of Tregs in the peripheral blood of children suffering from allergy tends to be higher whereas median of fluorescence intensity (MFI) of FoxP3 was significantly decreased in allergic group. Intracellular presence of regulatory cytokine interleukin (IL)-10 was also lower in allergic children. Immune functions of Tregs reflected by both MFI of FoxP3 and IL-10 in the group of probiotic-supplemented children of allergic mothers were nearly comparable with children of healthy mothers while probiotic non-supplemented children of allergic mothers have decreased immune function of Tregs. Supplementation by probiotic E. coli strain decreases allergy incidence in high-risk children. In contrast to our expectation, proportion of Tregs has not been increased in probiotic supplemented children. Beneficial effect of probiotics on newborn immature immune system could be, at least partially, explained by the modulating immune function of Tregs. In summary, we detected increased proportion of Tregs in peripheral blood of allergic children, their functional properties were decreased in comparison with the Tregs of healthy children. A unifying hypothesis for these findings is that Treg numbers in allergic children are increased in order to compensate for decreased function.

• MeSH
• alergie prevence a kontrola   MeSH
• cytokiny krev   imunologie   MeSH
• dítě MeSH
• Escherichia coli * MeSH
• interleukin-10 krev   imunologie   MeSH
• kojenec MeSH
• lidé MeSH
• novorozenec MeSH
• předškolní dítě MeSH
• probiotika aplikace a dávkování   MeSH
• průtoková cytometrie MeSH
• regulační T-lymfocyty imunologie   MeSH
• Check Tag
• dítě MeSH
• kojenec MeSH
• lidé MeSH
• mužské pohlaví MeSH
• novorozenec MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH