21584782 OR Molecular evidence of Plesiomonas shigelloides as a possible zoonotic agent
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The most frequently used method for establishing epidemiological relationships between Plesiomonas shigelloides strains is O:H serotyping. However, a number of strains are not serotypeable and isolates from diverse sources can display the same serovar. Moreover, since the zoonotic nature of Plesiomonas has been suggested and this hypothesis is based on the identical serovars found in animals and humans, we intend to use four DNA-based techniques: random amplified polymorphic DNA-PCR, enterobacterial repetitive intergenic consensus-PCR, repetitive extragenic palindromic-PCR, and pulsed field gel electrophoresis in order to screen 24 strains belonging to nine O:H serovars isolated from humans, animals, and the environment. In general, P. shigelloides showed a high genetic heterogeneity. Three pairs of strains, each containing a human and an animal isolate, displayed similar genotypes. This is the first report that provides molecular evidence that P. shigelloides may be zoonotic.
- MeSH
- genetická variace MeSH
- gramnegativní bakteriální infekce mikrobiologie přenos veterinární MeSH
- intergenová DNA MeSH
- lidé MeSH
- mikrobiologie životního prostředí MeSH
- molekulární typizace metody MeSH
- obrácené repetice MeSH
- Plesiomonas klasifikace genetika izolace a purifikace MeSH
- pulzní gelová elektroforéza metody MeSH
- repetitivní sekvence nukleových kyselin MeSH
- technika náhodné amplifikace polymorfní DNA metody MeSH
- zoonózy mikrobiologie přenos MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH