Fanconi anemia (FA) is a chromosomal instability disorder of bone marrow associated with aplastic anemia, congenital abnormalities and a high risk of malignancies. The identification of more than two dozen FA genes has revealed a plethora of interacting proteins that are mainly involved in repair of DNA interstrand crosslinks (ICLs). Other important findings associated with FA are inflammation, oxidative stress response, mitochondrial dysfunction and mitophagy. In this work, we performed quantitative proteomic and metabolomic analyses on defective FA cells and identified a number of metabolic abnormalities associated with cancer. In particular, an increased de novo purine biosynthesis, a high concentration of fumarate, and an accumulation of purinosomal clusters were found. This was in parallel with decreased OXPHOS and altered glycolysis. On the whole, our results indicate an association between the need for nitrogenous bases upon impaired DDR in FA cells with a subsequent increase in purine metabolism and a potential role in oncogenesis.
- MeSH
- buněčné linie MeSH
- chromatografie kapalinová MeSH
- Fanconiho anemie metabolismus MeSH
- glykolýza MeSH
- lidé MeSH
- metabolické sítě a dráhy * MeSH
- metabolomika metody MeSH
- oprava DNA MeSH
- oxidativní fosforylace MeSH
- proteomika metody MeSH
- tandemová hmotnostní spektrometrie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- glukoneogeneze účinky léků MeSH
- glukosa analýza metabolismus MeSH
- glykogen metabolismus MeSH
- glykogenolýza * MeSH
- glykolýza * MeSH
- hypoxie buňky MeSH
- indoly farmakologie MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- nádorové kmenové buňky metabolismus MeSH
- nádorové mikroprostředí MeSH
- oxidativní fosforylace MeSH
- propanoly farmakologie MeSH
- triple-negativní karcinom prsu metabolismus patologie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
