UNLABELLED: TIMP1 (tissue inhibitor of metalloproteinases 1) regulates extracellular matrix turnover and also promotes cell growth and has anti-apoptotic activity, which promotes malignant processes in tumor tissue. The aim of our study was to evaluate the relation of plasma TIMP1 protein levels with prognosis in patients with liver metastases, with particular regard to possible early-prediction of recurrence of the disease. PATIENTS AND METHODS: We studied a group of 87 patients with metastatic liver disease (mostly from colorectal cancer) who underwent surgery for liver metastases, and assessed their preoperative plasma TIMP1 levels. These levels were evaluated according to prognosis. Furthermore, we measured plasma TIMP1 in the post-operative period and tried to relate the changes with the diagnosis of relapse. RESULTS: We found preoperative plasma TIMP1 levels to be related to overall survival in the group of all patients with metastatic liver disease (p=0.0047), with a higher level being associated with an adverse outcome; the cut-off value was set at 165 ng/ml. This applied to all patients, regardless of the type of surgery. Assessment of the post-operative dynamics of TIMP1 was not found to be statistically significant to indicate disease recurrence. CONCLUSION: We found there to be a relationship between higher plasma levels of TIMP1 and an adverse prognosis in patients with liver metastases. The assessment of plasma TIMP1 levels could help the detection of patients with worse outcome.
- MeSH
- dospělí MeSH
- kolorektální nádory krev mortalita patologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- lokální recidiva nádoru krev mortalita MeSH
- nádorové biomarkery krev MeSH
- nádory jater krev mortalita sekundární chirurgie MeSH
- prognóza MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- tkáňový inhibitor metaloproteinasy 1 krev MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Cathepsins S and H are present in immune cells and tissues and may play a role in the activation of an adoptive immune response. Our goal was to assess their protein levels in pleural fluids from 82 patients who underwent thoracentesis or thoracoscopy for therapeutic or diagnostic reasons and to relate them to an inflammatory, neoplastic or hemodynamic origin. Pleural effusions were also analyzed for a panel of 13 inflammatory or proliferative markers to test possible links to a nonspecific host reaction. Increased levels of cathepsin S were found in parainflammatory and cancer-related effusions compared to transudates. Cathepsin H levels were elevated only in parainflammatory effusions, whereas the levels in cancer-related effusions were comparable to transudates. Cathepsin S values significantly correlated with LDH, alpha-1-AT, VEGF, sICAM, sVCAM, MPO, uPA, MMP-9/TIMP-1, IL-8 and MCP-1, but not with CRP, IL-10 or cathepsin H. In contrast to cathepsin S, cathepsin H values did not correlate with markers of inflammation, indicating a specific role for cathepsin H in the pleural host response. In conclusion, the estimation of cathepsin S and cathepsin H may help to distinguish between effusions of different etiology.
- MeSH
- biologické markery krev metabolismus MeSH
- cysteinové endopeptidasy krev metabolismus MeSH
- kathepsin H MeSH
- kathepsiny krev metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- maligní pleurální výpotek enzymologie MeSH
- nádorové biomarkery krev metabolismus MeSH
- nádory plic enzymologie MeSH
- paraneoplastické syndromy enzymologie MeSH
- pleurální výpotek enzymologie MeSH
- pleuritida enzymologie MeSH
- senioři MeSH
- zánět enzymologie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- práce podpořená grantem MeSH
OBJECTIVE: An anaerobic type of glycolysis exemplified by hyperproduction of the lactate dehydrogenase (LDH) subunit M has been detected in lung tumours, while a similar pattern has been found in concomitant pleural effusions (PE). The aim of this study was to verify the presence of the LDH subunit M in PEs of different aetiology and to compare its expression with markers of inflammation. MATERIAL AND METHODS: LDH isoenzymes were estimated and the LDH5/LDH1 coefficient was calculated in paraneoplastic PEs (n = 99), including subgroups with a different tumour ultrastructure, origin and pleural involvement. The expression pattern was compared with parainflammatory PEs (n = 21), transudates (n = 16) and with the expression of 13 inflammatory markers in PEs. RESULTS: The LDH5/LDH1 coefficient was higher in PEs associated with non-small-cell lung cancer (NSCLC) and with pleura-invading tumours, and lower in PEs of small-cell lung cancer and tumours without a confirmed pleural involvement. The LDH5/LDH1 coefficient positively correlated with uPA, IL-8, IL-10, sICAM, sVCAM, MPO and MMP-9. CONCLUSIONS: In accordance with inflammatory markers, it appears that the expression of LDH and its isoenzymes in PEs reflects the host reaction in pleural space and, in NSCLC, may also feature the anaerobic phenotype of cancer cells.
- MeSH
- biologické markery metabolismus MeSH
- financování organizované MeSH
- izoenzymy krev metabolismus MeSH
- L-laktátdehydrogenasa krev metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- mediátory zánětu analýza MeSH
- metastázy nádorů MeSH
- nádory plic enzymologie patologie ultrastruktura MeSH
- pleurální výpotek enzymologie etiologie MeSH
- podjednotky proteinů krev metabolismus MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
OBJECTIVE: Progelatinase B/proMMP-9 has recently been identified as an indicator of pleural inflammation, presumably originating from granulocytes. The aim of this study was to verify the origin of progelatinase B by simultaneous estimation of specific markers of neutrophil recruitment and activation in pleural effusions following induced pleurisy and pleural injury. MATERIAL AND METHODS: Sixty-three samples of pleural fluid from patients undergoing therapeutic talc pleurodesis (n = 8) and explorative thoracoscopy (n = 3) collected before and at different time intervals after the intervention were analyzed for progelatinase B and neutrophil gelatinase-associated lipocalin (NGAL)-gelatinase complex by substrate electrophoresis, for myeloperoxidase (MPO) and interleukin-8 (IL-8) by immunoadsorbent sandwich assay, as well as for leukocyte count, C-reactive protein (CRP) and total protein (TP). RESULTS: A significant increase in free and NGAL-complexed progelatinase B, MPO and IL-8 was recorded within 48 h following treatment in all subjects. Progelatinase B was strongly correlated with NGAL-gelatinase complex (r = 0.88, p = 0.001), MPO (r = 0.81, p = 0.001), neutrophil count (r = 0.75, p = 0.01) and IL-8 (r = 0.71, p = 0.001), but not with CRP and TP. CONCLUSIONS: The results support the neutrophil origin of the proenzyme, which confirms progelatinase B as an indicator of a local inflammatory reaction. Quantifying the inflammatory reaction may be helpful in the evaluation of both the technical variants of therapeutic pleurodesis and finer discrimination of paraneoplastic effusions.
- MeSH
- biologické markery metabolismus MeSH
- degranulace buněk fyziologie MeSH
- financování organizované MeSH
- interleukin-8 metabolismus MeSH
- kolagenasy biosyntéza MeSH
- lidé středního věku MeSH
- lidé MeSH
- lipokaliny MeSH
- matrixová metaloproteinasa 9 MeSH
- metaloendopeptidasy biosyntéza MeSH
- neutrofily enzymologie fyziologie patologie MeSH
- peroxidasa metabolismus MeSH
- pleura enzymologie patologie zranění MeSH
- pleurální výpotek enzymologie patologie MeSH
- pleuritida enzymologie patologie MeSH
- prekurzory enzymů biosyntéza MeSH
- proteiny akutní fáze metabolismus MeSH
- protoonkogenní proteiny metabolismus MeSH
- senioři MeSH
- želatinasy biosyntéza MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
Práce uvádí rozdělení a klasifikaci nejdůležitějších pneumokonióz (silikóza, uhlokopská pneumokonióza, azbestóza, pneumokonióza ze sváření, pneumokonióza z tvrdokovů), riziková pracoviště i nejčastější komplikace (plicní hypertenze, zánětlivá onemocnění plic, tuberkulóza, karcinom plic).
The authors analyze the classification of the most important pneumoconioses (silicosis, coal worker's pneumoconiosis, asbestosis, welder's pneumoconiosis, hard metal interstitial lung disease), risk workplaces and most frequent complications (pulmonary hypertension, inflammatory lung diseases, tuberculosis, lung cancer).
- MeSH
- lidé MeSH
- pneumokonióza diagnóza klasifikace patologie MeSH
- Check Tag
- lidé MeSH
