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Autor
Beraldo, Flavio 1 Calabrese, Evan 1 Caron, Marc G 1 Dzirasa, Kafui 1 Fan, Ping 1 Ginzel, Joshua D 1 Guzman Lenis, Monica S 1 Hatina, Jiri 1 Ichim, Gabriel 1 Johnson, G Allan 1 Kumar, Sunil 1 Nicholls, P J 1 Ostapchenko, Valeriy G 1 Pack, Thomas F 1 Prado, Marco A M 1 Prado, Vania F 1 Roberts, Wendy L 1 Snyder, Joshua C 1 Spasojevic, Ivan 1 Turu, Gabor 1
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Pracoviště
Center for In Vivo Microscopy Departm... 1 Center for Research on Cancer in Lyon... 1 Department of Anatomy and Cell Biolog... 1 Department of Biomedical Engineering ... 1 Department of Cell Biology Duke Unive... 1 Department of Medicine Duke Universit... 1 Department of Neurobiology Duke Unive... 1 Department of Pharmacology and Cancer... 1 Department of Physics Duke University... 1 Department of Physiology and Pharmaco... 1 Department of Psychiatry and Behavior... 1 Department of Surgery Duke University... 1 Institute of Biology Charles Universi... 1 Robarts Research Institute University... 1
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Autor
Beraldo, Flavio 1 Calabrese, Evan 1 Caron, Marc G 1 Dzirasa, Kafui 1 Fan, Ping 1 Ginzel, Joshua D 1 Guzman Lenis, Monica S 1 Hatina, Jiri 1 Ichim, Gabriel 1 Johnson, G Allan 1 Kumar, Sunil 1 Nicholls, P J 1 Ostapchenko, Valeriy G 1 Pack, Thomas F 1 Prado, Marco A M 1 Prado, Vania F 1 Roberts, Wendy L 1 Snyder, Joshua C 1 Spasojevic, Ivan 1 Turu, Gabor 1
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Pracoviště
Center for In Vivo Microscopy Departm... 1 Center for Research on Cancer in Lyon... 1 Department of Anatomy and Cell Biolog... 1 Department of Biomedical Engineering ... 1 Department of Cell Biology Duke Unive... 1 Department of Medicine Duke Universit... 1 Department of Neurobiology Duke Unive... 1 Department of Pharmacology and Cancer... 1 Department of Physics Duke University... 1 Department of Physiology and Pharmaco... 1 Department of Psychiatry and Behavior... 1 Department of Surgery Duke University... 1 Institute of Biology Charles Universi... 1 Robarts Research Institute University... 1
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PubMed Central od 2014
Europe PubMed Central od 2014
Open Access Digital Library od 2014-01-01
PubMed
36635920
DOI
10.1523/eneuro.0147-21.2022
Knihovny.cz E-zdroje
The protease caspase-3 is a key mediator of apoptotic programmed cell death. But weak or transient caspase activity can contribute to neuronal differentiation, axonal pathfinding, and synaptic long-term depression. Despite the importance of sublethal, or nonapoptotic, caspase activity in neurodevelopment and neural plasticity, there has been no simple method for mapping and quantifying nonapoptotic caspase activity (NACA) in rodent brains. We therefore generated a transgenic mouse expressing a highly sensitive and specific fluorescent reporter of caspase activity, with peak signal localized to the nucleus. As a proof of concept, we first obtained evidence that NACA influences neurophysiology in an amygdalar circuit. Then focusing on the amygdala, we were able to quantify a sex-specific persistent elevation in caspase activity in females after restraint stress. This simple in vivo caspase activity reporter will facilitate systems-level studies of apoptotic and nonapoptotic phenomena in behavioral and pathologic models.
- MeSH
- apoptóza * fyziologie MeSH
- kaspasa 9 MeSH
- mozek * MeSH
- myši transgenní MeSH
- myši MeSH
- neuroplasticita MeSH
- zvířata MeSH
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- mužské pohlaví MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
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