Nanoparticles offer targeted delivery of drugs with minimal toxicity to surrounding healthy tissue and have great potential in the management of human papillomavirus (HPV)-related diseases. We synthesized lipid-modified AS1411 aptamers capable of forming nanoaggregates in solution containing Mg2+. The nanoaggregates presented suitable properties for pharmaceutical applications such as small size (100 nm), negative charge, and drug release. The nanoaggregates were loaded with acridine orange derivative C8 for its specific delivery into cervical cancer cell lines and HPV-positive tissue biopsies. This improved inhibition of HeLa proliferation and cell uptake without significantly affecting healthy cells. Finally, the nanoaggregates were incorporated in a gel formulation with promising tissue retention properties aiming at developing a local delivery strategy of the nanoaggregates in the female genital tract. Collectively, these findings suggest that the nanoformulation protocol has great potential for the delivery of both anticancer and antiviral agents, becoming a novel modality for cervical cancer management.
- MeSH
- antitumorózní látky * chemie farmakokinetika farmakologie MeSH
- antivirové látky * chemie farmakokinetika farmakologie MeSH
- aptamery nukleotidové * chemie farmakokinetika farmakologie MeSH
- HeLa buňky MeSH
- lidé MeSH
- nádory děložního čípku farmakoterapie metabolismus MeSH
- oligodeoxyribonukleotidy * chemie farmakokinetika farmakologie MeSH
- proliferace buněk účinky léků MeSH
- systémy cílené aplikace léků * MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
