The ileal Peyers patches (IPP) of newborn germfree (GF) piglets were isolated into blind loops and the piglets colonized with a defined probiotic microflora. After 5 weeks, IgA levels in the intestinal lavage (IL) of loop piglets remained at GF levels and IgM comprised ∼70% while in controls, IgA levels were elevated 5-fold and comprised ∼70% of total Igs. Loop piglets also had reduced serum IgA levels suggesting the source of serum IgA had been interrupted. The isotype profile for loop contents was intermediate between that in the IL of GF and probiotic controls. Surprisingly, colonization alone did not result in repertoire diversification in the IPP. Rather, colonization promoted pronounced proliferation of fully switched IgA(+)IgM(-) B cells in the IPP that supply early, non-diversified "natural" SIgA antibodies to the gut lumen and a primary IgA response in serum.

• MeSH
• aktivace lymfocytů MeSH
• B-lymfocyty fyziologie   MeSH
• buněčná diferenciace MeSH
• gnotobiologické modely MeSH
• ileum imunologie   MeSH
• imunoglobulin A sekreční genetika   MeSH
• imunoglobulin M genetika   MeSH
• imunologická paměť MeSH
• kultivované buňky MeSH
• novorozená zvířata MeSH
• Peyerovy pláty imunologie   MeSH
• prasata imunologie   MeSH
• přesmyk imunoglobulinových tříd MeSH
• probiotika aplikace a dávkování   MeSH
• proliferace buněk MeSH
• rozmanitost protilátek MeSH
• střevní mikroflóra imunologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, U.S. Gov't, Non-P.H.S. MeSH