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Applied Molecular Virology Laboratory... 1 Department of Chemistry and Biochemis... 1 Department of Experimental Biology Fa... 1 Division of Bio Medical Science and T... 1 Institute of Parasitology Biology Cen... 1 KP Therapeutics s r o Purkyňova 649 1... 1 Screening Discovery Platform Institut... 1 Veterinary Research Institute Hudcova... 1
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Applied Molecular Virology Laboratory... 1 Department of Chemistry and Biochemis... 1 Department of Experimental Biology Fa... 1 Division of Bio Medical Science and T... 1 Institute of Parasitology Biology Cen... 1 KP Therapeutics s r o Purkyňova 649 1... 1 Screening Discovery Platform Institut... 1 Veterinary Research Institute Hudcova... 1
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- Duraisamy, Ganesh Selvaraj
- Jo, Eunji
- Huvarová, Ivana
- Park, Kyu-Ho P
- Heger, Zbyněk
- Adam, Vojtěch
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Růžek, Daniel, 1981-
Author Authority ORCID Veterinary Research Institute, Hudcova 296/70, CZ-621 00, Brno, Czech Republic Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 1160/31, CZ-370 05, České Budějovice, Czech Republic Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 735/5, CZ-625 00, Brno, Czech Republic
- Windisch, Marc P
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Miller, Andrew D
Author Miller, Andrew D Veterinary Research Institute, Hudcova 296/70, CZ-621 00, Brno, Czech Republic Department of Chemistry and Biochemistry, Mendel University in Brno, Zemědělská 1665/1, CZ-613 00, Brno, Czech Republic KP Therapeutics (Europe) s.r.o., Purkyňova 649/127, CZ-612 00, Brno, Czech Republic
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PubMed
36110238
DOI
10.1016/j.heliyon.2022.e10465
Knihovny.cz E-resources
Ginsenosides are a class of natural steroid glycosides and triterpene saponins found in Panax ginseng. After screening of a commercial ginsenoside compound library for low cellular cytotoxicity and the ability to mediate efficient reductions in hepatitis B virus (HBV) mRNA expression levels in HepG2.2.15 cells, three ginsenosides (Rg6, Rh4, and Rb3) are selected. Thereafter, using the same cellular model, all three ginsenosides are shown to mediate efficient, selective inhibition of HBV mRNA expression levels, and also interfere with the secretion of both HBV particles and hepatitis B surface antigen (HBsAg). Drug combination studies are performed in both HepG2.2.15 and HBV-infected HepG2-NTCPsec+ cell models with the selected ginsenosides and lamivudine (LMV), a nucleoside analogue used to treat chronic hepatitis B (CHB) infections. These studies, involving RT-qPCR and ELISA, suggest that Rh4/LMV combinations in particular act synergistically to inhibit the secretion of HBV particles and HBsAg. Therefore, on the assumption that appropriate in vivo data are in future agreement, Rh4, in particular, might be used in combination with nucleoside/nucleotide analogues (NUCs) to devise an effective, cost-efficient combination therapy for the treatment of patients with CHB infections.
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