Diamond-like carbon (DLC) is a biocompatible material that has many potential biomedical applications, including in orthopaedics. DLC layers doped with Cr at atomic percent (at.%) of 0, 0.9, 1.8, 7.3, and 7.7 at.% were evaluated with reference to their osteoinductivity with human bone marrow mesenchymal stromal cells (hMSCs), immune activation potential with RAW 264.7 macrophage-like cells, and their effect on apoptosis in Saos-2 human osteoblast-like cells and neonatal human dermal fibroblasts (NHDFs). At mRNA level, hMSCs on DLC doped with 0.9 and 7.7 at.% of Cr reached higher maximum values of both RUNX2 and alkaline phosphatase. An earlier onset of mRNA production of type I collagen and osteocalcin was also observed on these samples; they also supported the production of both type I collagen and osteocalcin. RAW 264.7 macrophages were screened using a RayBio™ Human Cytokine Array for cytokine production. 10 cytokines were at a concentration more than 2 × as high as the concentration of a positive control, but the values for the DLC samples were only moderately higher than the values on glass. NHDF cells, but not Saos-2 cells, had a higher expression of pro-apoptotic markers Bax and Bim and a lower expression of anti-apoptotic factor BCL-XL in proportion to the Cr content. Increased apoptosis was also proven by annexin V staining. These results show that a Cr-doped DLC layer with a lower Cr content can act as an osteoinductive material with relatively low immunogenicity, but that a higher Cr content can induce cell apoptosis.
- MeSH
- aktiny metabolismus MeSH
- alkalická fosfatasa genetika metabolismus MeSH
- apoptóza účinky léků imunologie MeSH
- buněčná adheze účinky léků MeSH
- buněčná diferenciace účinky léků imunologie MeSH
- buněčné linie MeSH
- chrom farmakologie MeSH
- cytokiny metabolismus MeSH
- diamant farmakologie MeSH
- fibroblasty cytologie účinky léků MeSH
- kolagen typu I genetika metabolismus MeSH
- lidé MeSH
- makrofágy účinky léků metabolismus MeSH
- mezenchymální kmenové buňky cytologie účinky léků imunologie metabolismus MeSH
- myši MeSH
- osteogeneze účinky léků MeSH
- osteokalcin genetika metabolismus MeSH
- počet buněk MeSH
- proliferace buněk účinky léků MeSH
- protein PEBP2alfaA genetika metabolismus MeSH
- regulace genové exprese účinky léků MeSH
- RNA metabolismus MeSH
- tvar buňky účinky léků MeSH
- vápník metabolismus MeSH
- vinkulin metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- financování organizované MeSH
- Publikační typ
- abstrakty MeSH