- MeSH
- endokarditida diagnóza farmakoterapie klasifikace prevence a kontrola MeSH
- hypertenze * diagnóza farmakoterapie klasifikace MeSH
- ischemická choroba srdeční diagnóza etiologie farmakoterapie klasifikace MeSH
- kardiomyopatie diagnóza klasifikace MeSH
- nemoci srdce * diagnóza etiologie farmakoterapie klasifikace MeSH
- nemoci srdečních chlopní diagnóza klasifikace terapie MeSH
- perikard patologie MeSH
- srdeční arytmie diagnóza etiologie klasifikace MeSH
- srdeční selhání diagnóza etiologie klasifikace MeSH
- Publikační typ
- přehledy MeSH
Pericardial fluid (PF) has been suggested as a reservoir of molecular targets that can be modulated for efficient repair after myocardial infarction (MI). Here, we set out to address the content of this biofluid after MI, namely in terms of microRNAs (miRs) that are important modulators of the cardiac pathological response. PF was collected during coronary artery bypass grafting (CABG) from two MI cohorts, patients with non-ST-segment elevation MI (NSTEMI) and patients with ST-segment elevation MI (STEMI), and a control group composed of patients with stable angina and without previous history of MI. The PF miR content was analyzed by small RNA sequencing, and its biological effect was assessed on human cardiac fibroblasts. PF accumulates fibrotic and inflammatory molecules in STEMI patients, namely causing the soluble suppression of tumorigenicity 2 (ST-2), which inversely correlates with the left ventricle ejection fraction. Although the PF of the three patient groups induce similar levels of fibroblast-to-myofibroblast activation in vitro, RNA sequencing revealed that PF from STEMI patients is particularly enriched not only in pro-fibrotic miRs but also anti-fibrotic miRs. Among those, miR-22-3p was herein found to inhibit TGF-β-induced human cardiac fibroblast activation in vitro. PF constitutes an attractive source for screening diagnostic/prognostic miRs and for unveiling novel therapeutic targets in cardiac fibrosis.
- MeSH
- fibroblasty metabolismus MeSH
- fibróza * MeSH
- infarkt myokardu s elevacemi ST úseků metabolismus patologie genetika MeSH
- infarkt myokardu * metabolismus genetika patologie MeSH
- interleukin-1 receptor-like 1 protein metabolismus genetika MeSH
- lidé středního věku MeSH
- lidé MeSH
- mikro RNA * genetika metabolismus MeSH
- myokard metabolismus patologie MeSH
- perikardiální tekutina * metabolismus MeSH
- senioři MeSH
- transformující růstový faktor beta metabolismus MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- fibrilace síní * chirurgie terapie MeSH
- kardiochirurgické výkony metody škodlivé účinky MeSH
- katetrizační ablace * metody MeSH
- lidé středního věku MeSH
- lidé MeSH
- perikard chirurgie MeSH
- senioři MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
BACKGROUND: Sodium-glucose cotransporter 2 inhibitors (SGLT-2i) are glucose-lowering agents used for the treatment of type 2 diabetes mellitus, which also improve heart failure and decrease the risk of cardiovascular complications. Epicardial adipose tissue (EAT) dysfunction was suggested to contribute to the development of heart failure. We aimed to elucidate a possible role of changes in EAT metabolic and inflammatory profile in the beneficial cardioprotective effects of SGLT-2i in subjects with severe heart failure. METHODS: 26 subjects with severe heart failure, with reduced ejection fraction, treated with SGLT-2i versus 26 subjects without treatment, matched for age (54.0 ± 2.1 vs. 55.3 ± 2.1 years, n.s.), body mass index (27.8 ± 0.9 vs. 28.8 ± 1.0 kg/m2, n.s.) and left ventricular ejection fraction (20.7 ± 0.5 vs. 23.2 ± 1.7%, n.s.), who were scheduled for heart transplantation or mechanical support implantation, were included in the study. A complex metabolomic and gene expression analysis of EAT obtained during surgery was performed. RESULTS: SGLT-2i ameliorated inflammation, as evidenced by the improved gene expression profile of pro-inflammatory genes in adipose tissue and decreased infiltration of immune cells into EAT. Enrichment of ether lipids with oleic acid noted on metabolomic analysis suggests a reduced disposition to ferroptosis, potentially further contributing to decreased oxidative stress in EAT of SGLT-2i treated subjects. CONCLUSIONS: Our results show decreased inflammation in EAT of patients with severe heart failure treated by SGLT-2i, as compared to patients with heart failure without this therapy. Modulation of EAT inflammatory and metabolic status could represent a novel mechanism behind SGLT-2i-associated cardioprotective effects in patients with heart failure.
- MeSH
- antiflogistika terapeutické užití farmakologie MeSH
- biologické markery krev MeSH
- diabetes mellitus 2. typu farmakoterapie metabolismus diagnóza MeSH
- epikardiální adipózní tkáň MeSH
- funkce levé komory srdeční účinky léků MeSH
- glifloziny * terapeutické užití farmakologie škodlivé účinky MeSH
- lidé středního věku MeSH
- lidé MeSH
- mediátory zánětu * metabolismus MeSH
- metabolomika MeSH
- perikard * metabolismus účinky léků MeSH
- srdeční selhání * metabolismus patofyziologie farmakoterapie MeSH
- stupeň závažnosti nemoci * MeSH
- tepový objem účinky léků MeSH
- tuková tkáň * účinky léků metabolismus MeSH
- výsledek terapie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- cysty mediastina * diagnóza patologie MeSH
- lidé MeSH
- perikard diagnostické zobrazování patologie MeSH
- senioři MeSH
- výsledek terapie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- Publikační typ
- kazuistiky MeSH
Zánět osrdečníku neboli perikarditida je relativně vzácné onemocnění srdce. V našich podmínkách bývají nejčastějším etiologickým agens viry. Mezi vzácnější agens patří Mycobacterium tuberculosis, typičtější spíše v rozvojových zemích. Komplikace nebývají časté, mezi nejčastější komplikace perikarditidy však patří vznik výpotku, rozvoj srdeční tamponády, konstriktivní perikarditida nebo rozvoj myoperikarditidy. Uvádíme případ 52letého pacienta, u něhož byla indikována totální perikardektomie pro tuberkulózní konstriktivní perikarditidu.
Pericarditis is a relatively rare heart disease. In our conditions, the most common etiological agent is viruses. Rarer agents include Mycobacterium tuberculosis, more typical in developing countries. Complications are not frequent, but the most common complications of pericarditis include the formation of an effusion, the development of cardiac tamponade, constrictive pericarditis or the development of myopericarditis. We present the case of a 52-year-old patient indicated for total pericardectomy for tuberculous constrictive pericarditis.
- MeSH
- dospělí MeSH
- elektrokardiografie metody MeSH
- konstriktivní perikarditida * diagnóza MeSH
- lidé MeSH
- Mycobacterium tuberculosis patogenita MeSH
- perikard chirurgie mikrobiologie patologie MeSH
- perikardektomie metody MeSH
- tuberkulóza mikrobiologie terapie virologie MeSH
- tuberkulózní perikarditida diagnóza MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
Introduction: Obesity is increasingly reported to be a contributing factor to vascular diseases resulting in increased patient morbidity and mortality rates leading to increase healthcare expenses. More precisely, the lipid deposition in cardiac tissues is interesting, due to their direct contribution to the disease initiation, prognosis, and all subsequent patients' fate. We do focus on the determination of the link between changes in body mass index (BMI) and epicardial fat deposition concerning gastric operation.Objective: The objective of this work was to study the relationship between weight reduction and "epicardial thickness" after laparoscopic-sleeve gastrectomy.Methods: Critically-ill patients were recruited from private clinics and out-patients hospital clinics. Forty-two patients undergoing laparoscopic-sleeve gastrectomy due to morbid obesity were included in this research. Patients followed for four months after the operation.Results: The results of this work find there is significant weight reduction in these patients in parallel with a reduction of epicardial fat thickness.Conclusion: Patients who experience laparoscopic-sleeve gastrectomy showed a reduction in weight in a parallel reduction in epicardial fat thickness.
- MeSH
- bariatrická chirurgie metody MeSH
- gastrektomie * metody MeSH
- hmotnostní úbytek MeSH
- kardiovaskulární nemoci etiologie MeSH
- laparoskopie metody MeSH
- lidé MeSH
- morbidní obezita chirurgie epidemiologie komplikace MeSH
- následné studie MeSH
- perikard * patologie MeSH
- tuková tkáň * chirurgie patologie MeSH
- výsledek terapie MeSH
- Check Tag
- lidé MeSH
(1) Background: C1q TNF-related protein 3 (CTRP3) is an adipokine with anti-inflammatory and cardioprotective properties. In our study, we explored changes in serum CTRP3 and its gene expression in epicardial (EAT) and subcutaneous (SAT) adipose tissue in patients with and without coronary artery disease (CAD) and type 2 diabetes mellitus (T2DM) undergoing elective cardiac surgery. (2) Methods: SAT, EAT, and blood samples were collected at the start and end of surgery from 34 patients: (i) 11 without CAD or T2DM, (ii) 14 with CAD and without T2DM, and (iii) 9 with both CAD and T2DM. mRNA levels of CTRP3 were assessed by quantitative reverse transcription PCR. Circulating levels of CTRP3 and other factors were measured using ELISA and Luminex Multiplex commercial kits. (3) Results: Baseline plasma levels of TNF-α and IL6 did not differ among the groups and increased at the end of surgery. Baseline circulating levels of CTRP3 did not differ among the groups and decreased after surgery. In contrast, baseline CTRP3 mRNA levels in EAT were significantly decreased in CAD/T2DM group, while no differences were found for TNF-α and IL6 gene expression. (4) Conclusions: Our data suggest that decreased EAT mRNA levels of CTRP3 could contribute to higher risk of atherosclerosis in patients with CAD and T2DM.
- MeSH
- diabetes mellitus 2. typu * komplikace genetika chirurgie MeSH
- interleukin-6 metabolismus MeSH
- kardiochirurgické výkony * MeSH
- lidé MeSH
- messenger RNA metabolismus MeSH
- nemoci koronárních tepen * komplikace genetika chirurgie MeSH
- perikard metabolismus MeSH
- TNF-alfa genetika metabolismus MeSH
- tuková tkáň metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
BACKGROUND: Cardiovascular surgery is confronted by a lack of suitable materials for patch repair. Acellular animal tissues serve as an abundant source of promising biomaterials. The aim of our study was to explore the bio-integration of decellularized or recellularized pericardial matrices in vivo. METHODS: Porcine (allograft) and ovine (heterograft, xenograft) pericardia were decellularized using 1% sodium dodecyl sulfate ((1) Allo-decel and (2) Xeno-decel). We used two cell types for pressure-stimulated recellularization in a bioreactor: autologous adipose tissue-derived stromal cells (ASCs) isolated from subcutaneous fat of pigs ((3) Allo-ASC and (4) Xeno-ASC) and allogeneic Wharton's jelly mesenchymal stem cells (WJCs) ((5) Allo-WJC and (6) Xeno-WJC). These six experimental patches were implanted in porcine carotid arteries for one month. For comparison, we also implanted six types of control patches, namely, arterial or venous autografts, expanded polytetrafluoroethylene (ePTFE Propaten® Gore®), polyethylene terephthalate (PET Vascutek®), chemically stabilized bovine pericardium (XenoSure®), and detoxified porcine pericardium (BioIntegral® NoReact®). The grafts were evaluated through the use of flowmetry, angiography, and histological examination. RESULTS: All grafts were well-integrated and patent with no signs of thrombosis, stenosis, or aneurysm. A histological analysis revealed that the arterial autograft resembled a native artery. All other control and experimental patches developed neo-adventitial inflammation (NAI) and neo-intimal hyperplasia (NIH), and the endothelial lining was present. NAI and NIH were most prominent on XenoSure® and Xeno-decel and least prominent on NoReact®. In xenografts, the degree of NIH developed in the following order: Xeno-decel > Xeno-ASC > Xeno-WJC. NAI and patch resorption increased in Allo-ASC and Xeno-ASC and decreased in Allo-WJC and Xeno-WJC. CONCLUSIONS: In our setting, pre-implant seeding with ASC or WJC had a modest impact on vascular patch remodeling. However, ASC increased the neo-adventitial inflammatory reaction and patch resorption, suggesting accelerated remodeling. WJC mitigated this response, as well as neo-intimal hyperplasia on xenografts, suggesting immunomodulatory properties.
- MeSH
- allogeneické buňky MeSH
- arteriae carotides MeSH
- cévní protézy MeSH
- hyperplazie MeSH
- lidé MeSH
- ovce MeSH
- perikard MeSH
- prasata MeSH
- remodelace cév * MeSH
- skot MeSH
- tkáňové inženýrství MeSH
- transplantace hematopoetických kmenových buněk * MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Kontext: Vzhledem k omezenému množství údajů stále ještě panuje značná nejistota ohledně významu a korelace mezi záhybem ušního lalůčku (earlobe crease, ELC) a zátěží koronárních tepen aterosklerózou plus adipozitou perikardu. Cíl: Cílem naší studie bylo zhodnotit souvislost mezi ELC a zátěží koronárních tepen aterosklerózou hodnocenou výpočtem kalciového skóre koronárních tepen (coronary artery calcium score, CAC), přítomností plátů a závažností koronárních stenóz i mezi ELC a objemem perikardiálního tuku (pericardial fat volume, PFV) u pacientů s podezřením na ischemickou chorobu srdeční. Metody: Do studie bylo zařazeno 70 pacientů odeslaných na naše oddělení pro klinické podezření na ischemickou chorobu srdeční, u nichž bylo provedeno vyšetření 64řádkovou CT koronarografií; z uvedeného počtu jich 33 (47 %) mělo ELC. Výsledky: Přítomnost ELC vykazovala po adjustaci na klasické faktory rizika vzniku koronárních příhod statisticky významně nezávislou korelaci se zvýšenou hodnotou CAC (OR [CI] = 3 [2-10]; p < 0,01), významnou koronární stenózou (OR [CI] = 3 [2-8]; p < 0,01) a s přítomností koronárních plátů (OR [CI] = 4 [3,5-9]; p < 0,01). Po adjustaci na klasické faktory rizika vzniku koronárních příhod byla přítomnost ELC (p < 0,01) nezávisle a významně spojena se zvýšenou hodnotou PFV. Závěr: Přítomnost ELC je významně a nezávisle spojena se zátěží aterosklerózy koronárních tepen a zvýšenou adipozitou perikardu.
Background: There is still uncertainty or limited data regarding the relevance of earlobe crease (ELC) to coronary atherosclerosis burden and pericardial adiposity. Objective: We aimed to assess the association between ELC with coronary atherosclerotic burden, including coronary artery calcium score (CAC), plaque presence, and coronary stenosis severity and pericardial fat volume (PFV) in patients with suspected coronary artery disease. Methods: Seventy patients referred owing to clinical suspicion of coronary artery disease who underwent 64-slice coronary CT angiography, 33 of whom (47%) had ELC, were enrolled. Results: ELC presence showed a significant independent association with increased CAC (OR [CI] = 3 [2-10], p <0.01), significant coronary stenosis (OR [CI] = 3 [2-8], p <0.01), and coronary plaque presence (OR [CI] = 4 [3.5-9], p <0.01) after adjustment for conventional coronary risk factors. ELC presence (p <0.01) was independently and significantly associated with increased PFV following adjustment for conventional coronary risk factors. Conclusion: ELC is significantly and independently associated with coronary atherosclerotic burden and in- creased pericardial adiposity.
- Klíčová slova
- ušní lalůček, záhyb ušního lalůčku,
- MeSH
- adipozita MeSH
- CT angiografie metody MeSH
- ischemická choroba srdeční diagnostické zobrazování patologie MeSH
- koronární angiografie metody MeSH
- lidé středního věku MeSH
- lidé MeSH
- nemoci koronárních tepen * diagnostické zobrazování patologie MeSH
- nemoci ucha epidemiologie etiologie MeSH
- perikard patologie MeSH
- průřezové studie MeSH
- regresní analýza MeSH
- rizikové faktory kardiovaskulárních chorob MeSH
- statistika jako téma MeSH
- zevní ucho * patologie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
