Melanoma is the least common form of skin tumor, but it is potentially the most dangerous and responsible for the majority of skin cancer deaths. We suggest that the skin microbiome might be changed during the progression of melanoma. The aim of this study is to compare the composition of the skin microbiota between different locations (skin and melanoma) of a MeLiM (Melanoma-bearing Libechov Minipig) pig model (exophytic melanoma). Ninety samples were used for PCR-DGGE analysis with primers specifically targeting the V3 region of the 16S rRNA gene. The profiles were used for cluster analysis by UPGMA and principal coordinate analysis PCoA and also to calculate the diversity index (Simpson index of diversity). By comparing the obtained results, we found that both bacterial composition and diversity were significantly different between the skin and melanoma microbiomes. The abundances of Fusobacterium and Trueperella genera were significantly increased in melanoma samples, suggesting a strong relationship between melanoma development and skin microbiome changes.

• MeSH
• Bacteria klasifikace   izolace a purifikace   MeSH
• DNA bakterií genetika   MeSH
• DNA primery MeSH
• Fusobacterium genetika   izolace a purifikace   MeSH
• genetická variace MeSH
• kůže mikrobiologie   MeSH
• melanom mikrobiologie   patologie   MeSH
• mikrobiota * MeSH
• miniaturní prasata MeSH
• modely nemocí na zvířatech MeSH
• prasata MeSH
• RNA ribozomální 16S genetika   MeSH
• sekvenční analýza DNA MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

Intercellular interactions are able to influence the biological properties of many types of tumors including malignant melanoma. Differentiation pattern of melanoma cells is significantly influenced by the melanoma-associated fibroblasts but the information about interaction of these cells with other important element of melanoma microenvironment, resp. with keratinocytes, is limited. In this, study we tested the effect of fibroblasts isolated from malignant melanoma on phenotype of normal human keratinocytes, especially on their expression of vimentin, a cytoskeletal protein weakly expressed in normal human keratinocytes. The co-culture with normal dermal fibroblasts was used for comparison. The results demonstrated the high expression of vimentin in keratinocytes co-cultured with melanoma-associated fibroblasts compared with those co-cultured with normal dermal fibroblasts. These data suggest participation of melanoma-associated fibroblasts-keratinocyte crosstalk in formation of melanoma niche.

• MeSH
• biomedicínský výzkum metody   MeSH
• fenotyp MeSH
• fibroblasty * cytologie   mikrobiologie   patologie   MeSH
• imunohistochemie metody   využití   MeSH
• interakce genů a prostředí MeSH
• keratinocyty * cytologie   enzymologie   imunologie   MeSH
• kultivační techniky metody   využití   MeSH
• lidé MeSH
• melanom * genetika   imunologie   mikrobiologie   MeSH
• mezibuněčná komunikace fyziologie   genetika   imunologie   MeSH
• nádorové buňky kultivované cytologie   imunologie   metabolismus   MeSH
• statistika jako téma MeSH
• vimentin imunologie   izolace a purifikace   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• práce podpořená grantem MeSH