Two new lung-dwelling nematode species of the genus Rhabdias Stiles et Hassall, 1905 were discovered in Caxiuanã National Forest, Pará state, Brazil. Rhabdias galactonoti sp. n. was found in a dendrobatid frog Adelphobates galactonotus (Steindachner). The species is characterised by the regularly folded inner surface of the anterior part of the buccal capsule seen in apical view, flask-shaped oesophageal bulb and narrow, elongated tail. Rhabdias stenocephala sp. n. from two species of leptodactylid frogs, Leptodactylus pentadactylus (Laurenti) (type host) and L. paraensis (Heyer), is characterised by a narrow anterior end that is separated from the remaining body by a constriction. Both species possess six small but distinct lips, a cuticle that is inflated along the whole body, a doliiform buccal capsule separated into a longer anterior and a shallow, ring-shaped posterior part, lateral pores in the body cuticle and zones of spermatogenesis in the syngonia. Rhabdias galactonoti sp. n. is the first species of the genus found in Dendrobatidae; R. stenocephala sp. n. is the second species described from Leptodactylidae in eastern Amazonia.
- MeSH
- druhová specificita MeSH
- plíce parazitologie MeSH
- Rhabditoidea anatomie a histologie klasifikace MeSH
- žáby parazitologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Brazílie MeSH
Based on material collected from Rhinella cf. margaritifera (Laurenti) and Rhi. marina (Linnaeus) (Anura: Bufonidae) during a parasite survey of the herpetofauna of French Guiana, updated descriptions of Rhabdias androgyna Kloss, 1971 and R. fuelleborni Travassos, 1926 are presented. In addition to metrical data, which may overlap in closely related species, emphasis is placed on qualitative characters. Rhabdias androgyna is distinguished by the unique presence of an outer and inner cephalic cuticular inflation, a shoulder-like broadening of the body at the anterior end, a wide and shallow buccal capsule (average buccal ratio 0.36) with serrated lumen in apical view, a prominent anterior dilatation of the oesophagus, and the presence of an additional posterior dilatation anterior to the oesophageal bulb. Characters that may help to differentiate R. fuelleborni from closely related species parasitising the Rhi. marina species group are the presence of six relatively uniform lips, and the division of the buccal capsule into an anterior and posterior segment, with differently structured walls. Both the presence of R. androgyna and R. fuelleborni in French Guiana constitute new geographic records. A single specimen of Rhabdias sp. is described from Pristimantis chiastonotus (Lynch et Hoegmood) (Anura: Craugastoridae). This species differs from all its Neotropical congeners by the distinct globular swelling of its head, similar to that seen in only one Palaearctic and one Afrotropical Rhabdias species. A list of species of Rhabdias parasitising amphibians in the Netropical Realm is also provided.
- MeSH
- plíce parazitologie MeSH
- Rhabdiasoidea * anatomie a histologie klasifikace MeSH
- ropuchy parazitologie MeSH
- žáby parazitologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Francouzská Guyana MeSH
Undercooked meat containing tissue cysts is one of the most common sources of Toxoplasma gondii infection in humans. Goats are very susceptible to clinical toxoplasmosis, and especially kids are common food animals, thereby representing a risk for human infection. A sequence-specific magnetic capture method was used for isolation of T. gondii DNA from tissue samples from experimentally infected goat-kids and real-time PCR for the 529 bp repeat element allowed quantification of T. gondii DNA. The contamination level in different types of tissue and in two groups of goats euthanized 30 and 90 dpi was compared. The highest concentration of T. gondii DNA in both groups of goats was found in lung tissue, but only the higher parasite count in lung tissue compared to other organs in group A (euthanized 30 dpi) was statistically significant. T. gondii concentrations were higher in liver and dorsal muscle samples from goats euthanized 90 dpi than in goats euthanized at 30 dpi, while the T. gondii concentration in hearts decreased. This study describes for the first time distribution of T. gondii parasites in post-weaned goat kids. New information about T. gondii predilection sites in goats and about the progression of infection between 30 and 90 dpi was achieved.
- MeSH
- játra parazitologie MeSH
- kosterní svaly parazitologie MeSH
- kozy MeSH
- kvantitativní polymerázová řetězová reakce metody veterinární MeSH
- magnetismus MeSH
- mozek parazitologie MeSH
- nemoci koz diagnóza parazitologie MeSH
- plíce parazitologie MeSH
- slezina parazitologie MeSH
- srdce parazitologie MeSH
- Toxoplasma izolace a purifikace MeSH
- toxoplazmóza zvířat diagnóza MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Thirty children aged from 2 to 15 with diagnosis “combined pulmonary and hepatic echinococcosis” were observed in the period from 2005 to 2011. Seventeen patients were undergone staging conventional echinococcectomy with interval not less than 2-3 weeks. Simultaneous endovisual echinococcectomy of the lung and liver was performed in 13 children. Our small experience showed that endosurgical intervention allows increasing volume of surgical intervention, excepting prolonged period between stages of echinococcectomy of the lung and liver. It shortens repetition factor of operative intervention, sufficiently decreases medicaments consumption, terms of staying in hospital, especially in postoperative period (7.2±1.2 b/d), terms of restoration of physical activity of patient, it gives a well cosmetic effect as well. Therefore, endovideosurgical echinococcectomy should take a priority in treatment of combined pulmonary and hepatic echinococcosis.
- MeSH
- arteria pulmonalis chirurgie parazitologie MeSH
- chirurgie operační metody využití MeSH
- dítě MeSH
- echinokokóza jater * chirurgie MeSH
- játra chirurgie parazitologie MeSH
- laparoskopie MeSH
- lidé MeSH
- mladiství MeSH
- plíce * chirurgie parazitologie MeSH
- předškolní dítě MeSH
- torakoskopie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
We have previously reported that Schistosoma mansoni larvae emerging from host lung at pH 7.5–7.8 and then fixed with diluted formaldehyde (HCHO) readily bind radiation-attenuated cercariae (RA) vaccine serum antibodies, as assessed by indirect membrane immunofluorescence (IF). Here we show that S. mansoni schistosomula emerging from lung pieces under 5% CO2 (pH ?7.3) readily bind RA vaccine serum antibodies, provided they have been incubated for 12 h at pH 7.5–7.8 in foetal calf serum-free RPMI medium, and fixed with diluted HCHO. Ex vivo larvae exposed during incubation to GW4869, a specific in-hibitor of tegument-bound, neutral sphingomyelinase (nSMase) displayed significantly diminished binding of RA vaccine serum antibodies, thus suggesting that nSMase activity at pH ?7.5 leads to exposure of lung-stage larvae surface membrane antigens to specific antibody detection. More importantly, ex vivo larvae readily bound antibodies directed to dipeptidic multiple antigen peptide constructs, based on S. mansoni-specific sequences in S. mansoni glyceraldehyde 3-phosphate dehydrogenase (SG3PDH). Lung-stage schistosomula IF reactivity was diminished following antiserum absorption with recombinant SG3PDH. The data together indicate that intact ex vivo, as well as, 5-day-old in vitro-grown larvae express SG3PDH on their surface mem-brane. The findings are discussed in relation to the importance of surface membrane proteins as candidate vaccine antigens.
- MeSH
- antigeny helmintové imunologie MeSH
- antigeny povrchové imunologie MeSH
- fluorescenční protilátková technika nepřímá MeSH
- glyceraldehyd-3-fosfátdehydrogenasa (NADP+) imunologie metabolismus MeSH
- křečci praví MeSH
- křeček rodu Mesocricetus MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- plíce parazitologie MeSH
- Schistosoma mansoni enzymologie MeSH
- schistosomiasis mansoni imunologie parazitologie MeSH
- zvířata MeSH
- Check Tag
- křečci praví MeSH
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- MeSH
- askarióza imunologie patologie veterinární MeSH
- imunologická odpověď na dávku MeSH
- larva MeSH
- plíce imunologie parazitologie MeSH
- počet parazitárních vajíček MeSH
- skot MeSH
- T-lymfocyty MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- Ascaris suum MeSH
- játra parazitologie MeSH
- larva migrans patologie veterinární MeSH
- ovce MeSH
- plíce parazitologie MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- MeSH
- imunologická odpověď na dávku MeSH
- makrofágy MeSH
- myši MeSH
- plíce cytologie imunologie parazitologie MeSH
- protilátky helmintové MeSH
- Toxocara canis imunologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
