Surface of ultra-high-molecular-weight polyethylene (UHMWPE) was modified by chemical methods. Surface was firstly activated by Piranha solution and then grafted with selected amino-compounds (cysteamine, ethylenediamine or chitosan). The next step was grafting of some borane cluster compounds, highly fluorescent borane hydride cluster anti-B18H22 or its thiolated derivative 4,4'-(HS)2-anti-B18H20. Polymer foils were studied using various methods to characterize surface chemistry (X-ray photoelectron spectroscopy), roughness and morphology (atomic force microscopy, scanning electron microscopy), chemistry and polarity (electrokinetic analysis), wettability (goniometry) and photophysical properties (UV-Vis spectroscopy) before and after modification steps. Subsequently some kinds of antimicrobial tests were performed. Immobilization of anti-B18H22 in small quantities onto UHMWPE surface leads to materials with a luminescence. Samples grafted with borane clusters showed significant inhibition of growth for gram-positive bacteria (S. epidermidis). These approaches can be used for (i) luminophores on the base of polymers nanocomposites development and/or (ii) preparation of materials with antimicrobial effects.
Poly(ethylene imine)s (PEIs) have been widely studied for biomedical applications, including antimicrobial agents against potential human pathogens. The interactions of branched PEIs (B-PEIs) with environmentally relevant microorganisms whose uncontrolled growth in natural or engineered environments causes health, economic, and technical issues in many sectors of water management are studied. B-PEIs are shown to be potent antimicrobials effective in controlling the growth of environmentally relevant algae and cyanobacteria with dual-functionality and selectivity. Not only did they effectively inhibit growth of both algae and cyanobacteria, mostly without causing cell death (static activity), but they also selectively flocculated cyanobacteria over algae. Thus, unmodified B-PEIs provide a cost-effective and chemically facile framework for the further development of effective and selective antimicrobial agents useful for control of growth and separation of algae and cyanobacteria in natural or engineered environments.
- MeSH
- antiinfekční látky chemie farmakologie MeSH
- Chlamydomonas reinhardtii růst a vývoj MeSH
- flokulace MeSH
- iminy * chemie farmakologie MeSH
- Microcystis růst a vývoj MeSH
- polyethyleny * chemie farmakologie MeSH
- Synechococcus růst a vývoj MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
We have investigated the application of Ar plasma for creation of nanostructured ultra high molecular weight polyethylene (PE) surface in order to enhance adhesion of mouse embryonic fibroblasts (L929). The aim of this study was to investigate the effect of the interface between plasma-treated and gold-coated PE on adhesion and spreading of cells. The surface properties of pristine samples and its modified counterparts were studied by different experimental techniques (gravimetry, goniometry and X-ray photoelectron spectroscopy (XPS), electrokinetic analysis), which were used for characterization of treated and sputtered layers, polarity and surface chemical structure, respectively. Further, atomic force microscopy (AFM) was employed to study the surface morphology and roughness. Biological responses of cells seeded on PE samples were evaluated in terms of cell adhesion, spreading, morphology and proliferation. Detailed cell morphology and intercellular connections were followed by scanning electron microscopy (SEM). As it was expected the thickness of a deposited gold film was an increasing function of the sputtering time. Despite the fact that plasma treatment proceeded in inert plasma, oxidized degradation products were formed on the PE surface which would contribute to increased hydrophilicity (wettability) of the plasma treated polymer. The XPS method showed a decrease in carbon concentration with increasing plasma treatment. Cell adhesion measured on the interface between plasma treated and gold coated PE was inversely proportional to the thickness of a gold layer on a sample.
- MeSH
- biokompatibilní potahované materiály * chemie farmakologie MeSH
- buněčné linie MeSH
- myši MeSH
- plazmové plyny chemie MeSH
- polyethyleny * chemie farmakologie MeSH
- povrchové vlastnosti MeSH
- testování materiálů * MeSH
- zlato * chemie farmakologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Interactions between maltose functionalized hyperbranched poly(ethylene imine)s (95% maltose decoration denoted as Mal-PEI A; 33% maltose decoration denoted as Mal-PEI B) and red blood cells (RBCs) and between red blood cell membranes were investigated. We monitored the degree of hemolysis, the change in cell shape, the influence of polymers on the fluidity of the cell membrane and some cell membrane enzymes to determine their possible cytotoxic impact on them. To observe the extent of hemolysis, the RBCs were incubated with different concentrations of Mal-PEIs. The first significant lysis of RBCs was observed after 6h of incubation. Prolongation of the incubation time increased the number of ruptured cells. Moreover, we observed that Mal-PEI B was more hemolytic than Mal-PEI A in buffer solution. In contrast, an incubation of RBCs with Mal-PEIs in human plasma significantly decreased the hemolytic process and showed higher hemolytic property of Mal-PEI A compared to Mal-PEI B. Also several changes in the shape of the RBCs occurred after incubation with Mal-PEIs. Some of the erythrocytes shrank (echinocytes), but their morphology generally remained unchanged during the incubation. As shown by fluorescence experiments, both polymers induced the increase of fluidity of RBCs membranes. In summary, both types of hyperbranched poly(ethylene imine)s were practically non-hemolytic even at high polymer concentrations. Mal-PEI B was slightly more noxious than the Mal-PEI A in a buffer solution, while in blood plasma, the situation was opposite. Decrease of Na+/K+ ATPase and total ATPase enzymes activity was related with molecule size and number of maltose groups on the surface of molecule. The low hemolytic properties only observed at higher concentration (100μM and 400μM) indicated that Mal-PEIs are promising macromolecules in the area of drug delivery systems.
- MeSH
- adenosintrifosfatasy metabolismus MeSH
- erytrocytární membrána účinky léků MeSH
- erytrocyty účinky léků MeSH
- fluorescenční spektrometrie MeSH
- iminy chemie farmakologie MeSH
- lidé MeSH
- maltosa chemie farmakologie MeSH
- nanočástice MeSH
- polyethyleny chemie farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Nanocomposites consisting of diatomaceous earth particles and silver nanoparticles (silver NPs) with high antimicrobial activity were prepared and characterized. For the purpose of nanocomposite preparation, silver NPs with an average size of 28nm prepared by modified Tollens process were used. Nanocomposites were prepared using poly(diallyldimethylammonium) chloride (PDDA) as an interlayer substance between diatomite and silver NPs which enables to change diatomite original negative surface charge to positive one. Due to strong electrostatic interactions between negatively charged silver NPs and positively charged PDDA-modified diatomite, Ag/PDDA-diatomite nanocomposites with a high content of silver (as high as 46.6mgAg/1g of diatomite) were prepared. Because of minimal release of silver NPs from prepared nanocomposites to aqueous media (<0.3mg Ag/1g of nanocomposite), the developed nanocomposites are regarded as a potential useful antimicrobial material with a long-term efficiency showing no risk to human health or environment. All the prepared nanocomposites exhibit a high bactericidal activity against Gram-negative and Gram-positive bacteria and fungicidal activity against yeasts at very low concentrations as low as 0.11g/L, corresponding to silver concentration of 5mg/L. Hence, the prepared nanocomposites constitute a promising candidate suitable for the microbial water treatment in environmental applications.
- MeSH
- antibakteriální látky chemická syntéza chemie farmakologie MeSH
- antifungální látky chemická syntéza chemie farmakologie MeSH
- Bacteria účinky léků MeSH
- křemelina chemie farmakologie MeSH
- kvartérní amoniové sloučeniny chemie farmakologie MeSH
- mikrobiální testy citlivosti MeSH
- nanokompozity chemie MeSH
- organokovové sloučeniny chemická syntéza chemie farmakologie MeSH
- polyethyleny chemie farmakologie MeSH
- povrchové vlastnosti MeSH
- Saccharomyces cerevisiae účinky léků MeSH
- stříbro chemie farmakologie MeSH
- velikost částic MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- vztahy mezi strukturou a aktivitou MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- dextrany farmakologie MeSH
- DNA účinky záření MeSH
- gelová chromatografie MeSH
- heparin farmakologie MeSH
- myši MeSH
- nukleoproteiny účinky záření MeSH
- polyethyleny farmakologie MeSH
- polyvinyly farmakologie MeSH
- radiační účinky MeSH
- slezina účinky léků účinky záření MeSH
- spektrofotometrie MeSH
- thymus účinky léků účinky záření MeSH
- tritium MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- MeSH
- bazofily MeSH
- Brucella MeSH
- cytoplazma MeSH
- histiocyty MeSH
- imunizace MeSH
- králíci MeSH
- kultivační média MeSH
- lymfocyty cytologie MeSH
- lymfoidní tkáň imunologie MeSH
- mitóza účinky léků MeSH
- plazmatické buňky MeSH
- polyethyleny farmakologie MeSH
- protilátky MeSH
- sérový albumin farmakologie MeSH
- vosky farmakologie MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
