We report that the cloned DNA harboring the long terminal repeat (LTR), v-src, LTR proviral structure is tumorigenic in chickens of the Prague congenic lines. The growth rate of these tumors is by far the highest in the recombinant CC.R1 line, the B haplotype of which is composed of the B-F/L4 and B-G12 subregions originating from different naturally occurring haplotypes. Some of the tumors induced by the LTR, v-src, LTR DNA are repeatedly transplantable in syngeneic chickens, maintain unaltered provirus, and express v-src mRNA. Differences in the response to challenge with Rous sarcoma virus (RSV) and LTR, v-src, LTR DNA on a given experimental model are compared and possible involvement of an interaction between B-F/L and B-G region genes is considered. Regression of the LTR, v-src, LTR DNA-induced tumors did not prevent the formation and growth of tumors induced subsequently by RSV.
- MeSH
- buněčné dělení genetika MeSH
- DNA nádorová fyziologie MeSH
- geny src fyziologie MeSH
- klonování DNA MeSH
- kur domácí MeSH
- nádory genetika mikrobiologie MeSH
- northern blotting MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- proviry MeSH
- repetitivní sekvence nukleových kyselin fyziologie MeSH
- Southernův blotting MeSH
- testy genetické komplementace MeSH
- viry ptačího sarkomu MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- DNA nádorová MeSH
Long-term persistence of the avian leukosis virus (ALV), the transformation-defective mutant of Prague strain Rous sarcoma virus subgroup C (td PR-C) was established in heterologous duck hosts after infection in mid-embryogenesis. Transient viraemia was observed for about 4 weeks after hatching and was lost in most of the infected ducks by about 6 months. Loss of viraemia was accompanied by the increasing synthesis of virus-neutralizing antibodies. In spite of strong virus-neutralizing antibodies, virus was detected by the cocultivation assay in duck tissues throughout the observation period up to 5 years. In the viraemic phase of infection, we found integrated proviruses in various tissues, preferentially in stomach muscle tissue and in the thymus. The long-term persistence of virus was frequently accompanied by liver necrosis and neoplastic diseases. Injection of td PR-C virus into early embryos resulted in more pronounced infection accompanied by an increased copy number of viral DNA per cell, high mortality and remarkable atrophy of thymus tissue in infected ducklings.
- MeSH
- DNA virů genetika izolace a purifikace MeSH
- hybridizace nukleových kyselin MeSH
- kachny mikrobiologie MeSH
- molekulová hmotnost MeSH
- neutralizační testy MeSH
- protilátky virové analýza MeSH
- ptačí leukóza imunologie patofyziologie MeSH
- restrikční mapování MeSH
- viremie patofyziologie MeSH
- virus ptačí leukózy genetika patogenita MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- DNA virů MeSH
- protilátky virové MeSH
The LTR, v-src, LTR provirus, which arose by the reverse transcription and integration of src mRNA in the H-19 hamster tumor, has been successfully rescued by fusion with chicken fibroblasts infected with Rous-associated virus RAV-1. One rescued virus, E6, acquired 1 kilobase of the 5' end of the gag gene structure. Recombination took place in the region of 15-nucleotide homology exactly between v-src exon (position 7054) and gag (position 1417). This recombination resulted in the alteration of src splice acceptor site sequences, but this site is maintained as a functional splice acceptor site. The nucleotide structure of the long terminal repeat of recombinant E6 virus suggests that it arose by the intermolecular jump of reverse transcription from RAV-1 to src mRNA and then the switch of templates between already depicted regions of homology. The second jump of reverse transcription was apparently an intramolecular event. The acquisition of 1 kilobase of the 5' gag by E6 resulted in maintaining the balance of unspliced and spliced E6 RNAs and assured the replication advantage of rescued E6 virus over rescued F6 virus, the genome of which corresponds to that present in ancestral H-19 cells.
- MeSH
- geny gag MeSH
- messenger RNA genetika MeSH
- molekulární sekvence - údaje MeSH
- onkogeny * MeSH
- proviry genetika MeSH
- rekombinace genetická MeSH
- repetitivní sekvence nukleových kyselin MeSH
- RNA virová genetika MeSH
- sekvence nukleotidů MeSH
- virová transformace buněk genetika MeSH
- virus ptačí leukózy genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Názvy látek
- messenger RNA MeSH
- RNA virová MeSH
