The concentrations of immunoglobulin E (IgE) were measured serially in 30 patients with severe burn injury. A characteristic finding was a rapid and dramatic increase of IgE in the serum of all patients, some of whom showed up to 20-fold increases. IgE was also found in considerable amounts in blister fluid and pleural effusions. In some patients raised IgE values were the only indicator of an anamnestic immediate allergic state. No correlation was found between the IgE levels and clinical characteristics of individual patients but significant changes seemed to be present only in patients with a burn covering more than 20 per cent of the body surface area.

• MeSH
• časové faktory MeSH
• dítě MeSH
• dospělí MeSH
• ELISA MeSH
• exsudáty a transsudáty imunologie   MeSH
• imunoglobulin E analýza   MeSH
• lidé MeSH
• pleurální výpotek imunologie   MeSH
• popálení imunologie   MeSH
• puchýř MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• imunoglobulin E MeSH

Peritoneal exudate cells (PEC) from conventional donors displayed in vitro the cytotocix effects on the rat sarcoma cell lines LWI3K2, LW13K3, LW13K4 and RSL but they were not cytotoxic to fibroblasts derived from normal rats of the same strains. PEC were also cytotoxic to human urinary bladder carcinoma cells (T-24) grown in vitro. Cytotoxicity was detectable already at the effector to target cell ratio of 1 : 1, and the effector cells from syngeneic, allogeneic and xenogeneic donors were equally effective. The cytotoxic effect was apparently exerted by macrophages because the addition of lymphocytes from PEC donors did not change the target cells. The addition of serum from PEC donors to a mixture of target and effector cells protected the tumour cells from the destruction by macrophages. Cytotoxicity was blocked by serum from all PEC donors tested (syngeneic, allogeneic and xenogeneic with regard to the target cells) but not by foetal calf serum.

• MeSH
• buněčné linie MeSH
• cytotoxické testy imunologické * MeSH
• exsudáty a transsudáty imunologie   MeSH
• fibroblasty imunologie   MeSH
• inbrední kmeny myší imunologie   MeSH
• makrofágy imunologie   MeSH
• myši MeSH
• nádory močového měchýře imunologie   MeSH
• ptačí sarkom imunologie   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

Administration of proteose peptone to donors of peritoneal exudate cells resulted not only in quantitative increase in the mono-histiocytic cells but also in their activation as judged by their rapid adherence to surfaces, strong acid phosphatase reaction and increased numbers of lysosomes and phagocytic vacuoles. The three-hour adherent fraction of peritoneal exudate cells (from normal as well as peptone-stimulated mice) contains a large number of activated lymphocytes and fibroblastoid cells and is not enriched in macrophages. Moreover, the adherent cells, when collected by scraping, are unsuitable for immediate biological use since they lose the enzymatic equipment. For purification of histiocytes and macrophages by adherence, the 2-day cultivation of peritoneal cells was found to be optimal, as judged by the number of acid phosphatase positive cells. Their decrease after 4-day cultivation may indicate over-growth of the fibroblast and epithelial cell lineages. The non-adherent fraction was found to be enriched in lymphocytes as well as in mast cells; numerous macrophages were observed, too.

• MeSH
• exsudáty a transsudáty imunologie   ultrastruktura   MeSH
• granulocyty imunologie   MeSH
• histiocyty imunologie   MeSH
• imunoadherenční reakce MeSH
• lymfocyty imunologie   MeSH
• makrofágy imunologie   MeSH
• mastocyty imunologie   MeSH
• monocyty imunologie   MeSH
• myši MeSH
• peritoneum imunologie   ultrastruktura   MeSH
• tvorba protilátek MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• antinukleární protilátky analýza   MeSH
• artritida imunologie   MeSH
• autoprotilátky analýza   MeSH
• exsudáty a transsudáty imunologie   MeSH
• fluorescenční protilátková technika MeSH
• lidé středního věku MeSH
• lidé MeSH
• ulcerózní kolitida imunologie   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antinukleární protilátky MeSH
• autoprotilátky MeSH