Control of common scab disease can be reached by resistant cultivars or suppressive soils. Both mechanisms are likely to translate into particular potato microbiome profiles, but the relative importance of each is not known. Here, microbiomes of bulk and tuberosphere soil and of potato periderm were studied in one resistant and one susceptible cultivar grown in a conducive and a suppressive field. Disease severity was suppressed similarly by both means yet, the copy numbers of txtB gene (coding for a pathogenicity determinant) were similar in both soils but higher in periderms of the susceptible cultivar from conducive soil. Illumina sequencing of 16S rRNA genes for bacteria (completed by 16S rRNA microarray approach) and archaea, and of 18S rRNA genes for micro-eukarytes showed that in bacteria, the more important was the effect of cultivar and diversity decreased from resistant cultivar to bulk soil to susceptible cultivar. The major changes occurred in proportions of Actinobacteria, Chloroflexi, and Proteobacteria. In archaea and micro-eukaryotes, differences were primarily due to the suppressive and conducive soil. The effect of soil suppressiveness × cultivar resistance depended on the microbial community considered, but differed also with respect to soil and plant nutrient contents particularly in N, S and Fe.

• MeSH
• Actinobacteria klasifikace   genetika   růst a vývoj   patogenita   MeSH
• Archaea klasifikace   genetika   růst a vývoj   patogenita   MeSH
• Chloroflexi klasifikace   genetika   růst a vývoj   patogenita   MeSH
• dusík metabolismus   farmakologie   MeSH
• eukaryotické buňky metabolismus   MeSH
• faktory virulence genetika   metabolismus   MeSH
• genotypizační techniky MeSH
• mikrobiota genetika   MeSH
• náchylnost k nemoci imunologie   MeSH
• nemoci rostlin imunologie   mikrobiologie   MeSH
• odolnost vůči nemocem účinky léků   MeSH
• Proteobacteria klasifikace   genetika   růst a vývoj   patogenita   MeSH
• půdní mikrobiologie * MeSH
• RNA ribozomální 16S genetika   MeSH
• RNA ribozomální 18S genetika   MeSH
• síra metabolismus   farmakologie   MeSH
• Solanum tuberosum účinky léků   imunologie   mikrobiologie   MeSH
• železo metabolismus   farmakologie   MeSH
• zemědělské plodiny MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• dusík MeSH
• faktory virulence MeSH
• RNA ribozomální 16S MeSH
• RNA ribozomální 18S MeSH
• síra MeSH
• železo MeSH

Bacterial clone libraries of the gut microbiota of nurtured and starved Cylindroiulus fulviceps specimens displayed the predominance of the phyla Bacteroidetes (55 and 37 %, respectively) and Proteobacteria (40 and 35 %, respectively) and a high similarity to bacteria previously detected in the intestinal tract of termites and beetles, which are known to harbor symbiotic bacteria essential for digestive activity. Bacterial isolates were dominated by Proteobacteria (74 %), followed by members of the phyla Actinobacteria, Firmicutes and Bacteroidetes. PCR-DGGE fingerprints of the gut samples showed that intestinal bacteria were affected by starvation, although the change was not significant.

• MeSH
• Actinobacteria klasifikace   genetika   izolace a purifikace   MeSH
• Bacteria klasifikace   genetika   izolace a purifikace   MeSH
• Bacteroidetes klasifikace   genetika   izolace a purifikace   MeSH
• bakteriologické techniky MeSH
• členovci mikrobiologie   MeSH
• DNA bakterií genetika   MeSH
• DNA fingerprinting metody   MeSH
• ekosystém MeSH
• elektroforéza v agarovém gelu metody   MeSH
• fylogeneze MeSH
• gastrointestinální trakt mikrobiologie   MeSH
• klonování DNA metody   MeSH
• kultivační média MeSH
• metagenom MeSH
• molekulární sekvence - údaje MeSH
• Proteobacteria klasifikace   genetika   izolace a purifikace   MeSH
• RNA ribozomální 16S genetika   MeSH
• sekvenční analýza DNA MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DNA bakterií MeSH
• kultivační média MeSH
• RNA ribozomální 16S MeSH

As part of the EU project MULTIBARRIERS, six new endogenous aerobic bacterial isolates able to grow in the presence of BTmX (benzene, toluene, m-xylene) were characterized with respect to their growth specificities. Preliminary analysis included restriction fragment length polymorphism profiles and 16S rDNA sequencing. The diversity of these strains was confirmed by denaturing gradient gel electrophoresis. Additional aerobic bacterial strains were isolated from the rhizospheres of plants grown in polychlorinated biphenyl (PCB)-contaminated soils. Pot experiments were designed to show the beneficial effect of plants on the bacterial degradation of PCBs. The effect of PCB removal from soil was evaluated and bacteria isolated from three different plant species were examined for the presence of the bph operon.

• MeSH
• Actinobacteria klasifikace   genetika   metabolismus   MeSH
• aromatické uhlovodíky metabolismus   MeSH
• biodegradace MeSH
• chemické látky znečišťující vodu metabolismus   MeSH
• dekontaminace metody   MeSH
• ekosystém MeSH
• kořeny rostlin mikrobiologie   MeSH
• látky znečišťující půdu metabolismus   MeSH
• Proteobacteria klasifikace   genetika   metabolismus   MeSH
• půdní mikrobiologie MeSH
• rostliny mikrobiologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• aromatické uhlovodíky MeSH
• chemické látky znečišťující vodu MeSH
• látky znečišťující půdu MeSH

A selective Proteeae medium (SPM) for isolation and preliminary detection of species of genera Proteus, Morganella, and Providencia was evaluated. The SPM contains tryptose phosphate agar with phenolphthalein monophosphate (as substrate for phosphatase activity), bile salts and polymyxin B (as inhibitors). The selectivity of the SPM was tested by the ecometric method of quality assurance of culture media. Fourteen reference cultures of enterobacteria and fifty-four strains of Proteeae were tested for their absolute growth index (AGI). Ninety-five percent of tested Proteeae strains display an AGI above 2.5. The detected phosphatase activity proved to be able to discriminate colonies of members of the tribe Proteeae. The ability of SPM for primary isolation of members of Proteeae was tested on food and clinical material and 94 strains were isolated. In addition, the SPM was employed in routine practice of clinical microbiology. From 1016 clinical samples (stool, urine, vaginal and urethral swabs), 57 strains of Proteeae were detected by the SPM in contrast to 35 strains by the routine procedure. The difference amounts to nearly 40%.

• MeSH
• bakteriální infekce mikrobiologie   MeSH
• druhová specificita MeSH
• infekce spojené se zdravotní péčí mikrobiologie   MeSH
• kultivační média * chemie   MeSH
• lidé MeSH
• Morganella růst a vývoj   izolace a purifikace   MeSH
• potravinářská mikrobiologie MeSH
• Proteobacteria klasifikace   růst a vývoj   izolace a purifikace   MeSH
• Proteus růst a vývoj   izolace a purifikace   MeSH
• Providencia růst a vývoj   izolace a purifikace   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• Názvy látek
• kultivační média * MeSH