Cellular activities in the regulation of growth or adhesion/migration involve protein (lectin)-carbohydrate recognition at the cell surface. Members of the galectin family of endogenous lectins additionally bind distinct intracellular ligands. These interactions with protein targets explain the relevance of their nuclear and cytoplasmic presence. Expression profiling for galectins and accessible binding sites is a histochemical approach to link localization with cellular growth properties. Non-cross-reactive antibodies for the homodimeric (proto-type) galectins-1, -2 and -7 and the chimera-type galectin-3 (Gal-3) as well as the biotinylated lectins were tested. This analysis was performed with the FaDu squamous carcinoma cell line and long-term cultured human and porcine epidermal cells as models for malignant and normal cells of squamous cell epithelial origin. A set of antibodies was added for phenotypic cell characterization. Strong nuclear and cytoplasmic signals of galectins and the differential reactivity of labeled galectins support the notion of their individual properties. The length of the period of culture was effective in modulating marker expression. Cytochemical expression profiling is a prerequisite for the selection of distinct proteins for targeted modulation of gene expression as a step toward functional analysis.

• MeSH
• buněčná diferenciace fyziologie   MeSH
• buněčné jádro metabolismus   ultrastruktura   MeSH
• epitelové buňky metabolismus   MeSH
• fenotyp MeSH
• galektiny metabolismus   MeSH
• lidé MeSH
• molekuly buněčné adheze metabolismus   MeSH
• prasata MeSH
• růstové látky metabolismus   MeSH
• sacharidy fyziologie   MeSH
• signální transdukce fyziologie   MeSH
• spinocelulární karcinom metabolismus   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• galektiny MeSH
• molekuly buněčné adheze MeSH
• růstové látky MeSH
• sacharidy MeSH

Craniopharyngioma is a rare benign tumour originating from Rathke's pouch. This paper reports a tumour case studied with a set of markers defining protein-carbohydrate recognition. Expression of endogenous lectins and their reactive glycoligands is under differentiation-dependent control in many cell types. These parameters can be related to the degree of cell differentiation in tumours. Therefore, the expression patterns of endogenous lectins, namely galectins-1, -3, and -7, in the craniopharyngioma case were determined. Galectins-1 and -3 were also used to reveal glycoconjugates in cells and extracellular matrices, an approach that has heretofore relied largely on plant lectins. The staining pattern of craniopharyngioma is compared with that of two other types of ectodermally derived tumours, namely basal and squamous cell carcinomas. Clusters of polygonal and flattened cells with morphological characteristics of differentiated cells in the craniopharyngioma and the majority of poorly differentiated cells in squamous cell carcinomas were reactive with galectin-3. No binding of this probe was observed in cells of basal cell carcinomas and the majority of craniopharyngioma cells. In view of the lack of accessible binding in the basal layer of normal squamous epithelia where proliferative cells (including stem cells) are located, galectin-3 binding could be used to distinguish basal from suprabasal cells of squamous epithelial cells.

• MeSH
• bazocelulární nádory metabolismus   patologie   MeSH
• buněčná diferenciace fyziologie   MeSH
• dospělí MeSH
• epitel metabolismus   patologie   MeSH
• epitopy MeSH
• fenotyp MeSH
• fluorescenční mikroskopie MeSH
• galektiny metabolismus   MeSH
• histocytochemie MeSH
• keratiny metabolismus   MeSH
• kraniofaryngeom metabolismus   patologie   MeSH
• lektiny MeSH
• lidé MeSH
• monoklonální protilátky MeSH
• nádorové biomarkery MeSH
• nádory hypofýzy metabolismus   patologie   MeSH
• polysacharidy metabolismus   MeSH
• spinocelulární karcinom metabolismus   patologie   MeSH
• vazebná místa MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• epitopy MeSH
• galektiny MeSH
• keratiny MeSH
• lektiny MeSH
• monoklonální protilátky MeSH
• nádorové biomarkery MeSH
• polysacharidy MeSH

BACKGROUND/AIM: Components of the tear fluid contribute to the biochemical defence system of the eye. To reveal whether the immune mediator and lipopolysaccharide binding galectin-3 is present in tears, tear samples were collected from eyes in healthy and pathological states. Investigation of expression of galectin-3 and galectin-3 reactive glycoligands in normal human conjunctival and corneal epithelia was also initiated as a step to understand the role of galectin-3 in ocular surface pathology. METHODS: Immunoblot analysis using either a rabbit polyclonal or a mouse monoclonal antibody against galectin-3 was employed to detect galectin-3 in tear fluid. Galectin-3 expression in tissue specimens was detected by immunocytochemistry employing A1D6 mouse monoclonal antibody, and galectin-3 reactive glycoligands were visualised by lectin histochemistry using labelled galectin-3. RESULTS: Galectin-3 was found only in tears from patients with ocular surface disorders. It was expressed in normal corneal and conjunctival epithelia but not in lacrimal glands. Inflammatory leucocytes and goblet cells found in galectin-3 containing tear fluid also expressed galectin-3. Galectin-3 binding sites were detected on the surface of conjunctival and corneal epithelial cells co-localising with desmoglein. CONCLUSIONS: This study revealed expression of galectin-3 in tear fluid obtained from patients with eye diseases. The role of this endogenous lectin (produced by inflammatory as well as epithelial cells) in antimicrobial action and inflammation modulation could be expected.

• MeSH
• diferenciační antigeny analýza   MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• fluorescenční protilátková technika MeSH
• galektin 3 MeSH
• imunoblotting MeSH
• konjunktiva metabolismus   MeSH
• leukocyty metabolismus   MeSH
• lidé MeSH
• luminiscenční měření MeSH
• oční nemoci metabolismus   MeSH
• pohárkové buňky metabolismus   MeSH
• rohovkový epitel metabolismus   MeSH
• slzy chemie   MeSH
• western blotting MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• diferenciační antigeny MeSH
• galektin 3 MeSH