The bodies of primary sensory neurons and their satellite glial cells (SGCs) are limited by the basal laminae from extracellular matrix of the dorsal root ganglia (DRG). The basal laminae displayed uniform immunofluorescence staining for laminin-1 in the sections of rat intact (naive) DRG. A proximal or distal ligature of the spinal nerves resulted in a heterogeneous immunostaining for laminin-1 around neuron-SGC units in the sections of the corresponding DRG. The pattern of irregular laminin-1 immunofluorescence was more extensive in the ipsilateral than the contralateral DRG of the operated rats. The immunofluorescence for laminin-1 exactly coincided with binding of Concanavalin-A as well as immunostaining for type IV collagen in both naive DRG and DRG affected by nerve ligature. Nidogen immunostaining decreased or fully disappeared at the surface of the SGCs consistently with immunofluorescence staining for laminin-1, but retained or increased in the endothelial cells and ED-1 positive cells invaded the DRG affected by nerve ligature. The results indicate an alteration of the content of basal laminae surrounding the bodies of primary sensory neurons and their SGSs following nerve constriction injury. A modulation of the basal laminae may be related with other cellular and molecular alterations related with peripheral neuropathic pain, for example, expansion of sympathetic sprouts.

• MeSH
• Staining and Labeling methods   MeSH
• Basement Membrane chemistry   MeSH
• Ectodysplasins analysis   MeSH
• Fluorescent Antibody Technique methods   MeSH
• Collagen Type IV analysis   MeSH
• Concanavalin A analysis   MeSH
• Rats MeSH
• Laminin analysis   MeSH
• Membrane Glycoproteins analysis   MeSH
• Neuroglia chemistry   pathology   MeSH
• Rats, Wistar MeSH
• Ganglia, Spinal chemistry   injuries   pathology   MeSH
• Constriction, Pathologic MeSH
• Animals MeSH
• Check Tag
• Rats MeSH
• Female MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Ectodysplasins MeSH
• Collagen Type IV MeSH
• Concanavalin A MeSH
• laminin 1 MeSH Browser
• Laminin MeSH
• Membrane Glycoproteins MeSH
• nidogen MeSH Browser