A new simple method used to eliminate polysaccharides that cause problems during DNA isolation was established for 6 different white-rot fungi using 1% hexadecyltrimethylammonium bromide (CTAB) as wash buffer and followed by centrifugation. Variation in the DNA yield and quality was ascertained using precipitating agents, detergents and cell-wall-hydrolyzing chitinase. Considerable amount of exopolysaccharides from fungal biomass was removed with the use of 1% CTAB wash buffer followed by centrifugation. The DNA varied in terms of yield and quality. For the DNA extraction use of 2% SDS in extraction buffer worked best for Pycnoporus cinnabarinus, Cyathus bulleri, Cyathus striatus and Cyathus stercoreus, while 2% CTAB worked best for Phanerochaete chrysosporium and Pleurotus ostreatus. Elimination of phenol and use of absolute ethanol for precipitating DNA resulted in good yield and quality of DNA. This DNA was amenable to restriction endonuclease digestion.

• MeSH
• Basidiomycota chemie   MeSH
• cetrimoniové sloučeniny chemie   MeSH
• cetrimonium MeSH
• chitinasy metabolismus   MeSH
• detergenty MeSH
• DNA fungální analýza   izolace a purifikace   metabolismus   MeSH
• fenol chemie   MeSH
• frakční precipitace MeSH
• mykologie metody   MeSH
• Phanerochaete chemie   MeSH
• Pleurotus chemie   MeSH
• polysacharidy chemie   MeSH
• restrikční enzymy metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• cetrimoniové sloučeniny MeSH
• cetrimonium MeSH
• chitinasy MeSH
• detergenty MeSH
• DNA fungální MeSH
• fenol MeSH
• polysacharidy MeSH
• restrikční enzymy MeSH

Eighty-three strains belonging to three species of the genus Trametes FR. (T. versicolor, T. hirsuta and T. ochracea) collected in different localities and on different substrates were screened for laccase production. The production of other lignin-modifying enzymes--manganese peroxidase (MnP) and lignin peroxidase (LiP)--and the decolorization ability were also determined in 21 of them. Production variability was relatively high and no significant correlation was found between the origin of the strains (locality, substrate) and the enzyme production. Dikaryons of all 3 species (but not of all their strains) exhibited LiP activity, which was not detected in the respective monokaryons.

• MeSH
• Basidiomycota enzymologie   MeSH
• lakasa MeSH
• lignin metabolismus   MeSH
• metoda Monte Carlo MeSH
• multivariační analýza MeSH
• nadmořská výška MeSH
• oxidoreduktasy izolace a purifikace   metabolismus   MeSH
• peroxidasy izolace a purifikace   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Bulharsko MeSH
• Česká republika MeSH
• Jugoslávie MeSH
• Názvy látek
• lakasa MeSH
• lignin peroxidase MeSH Prohlížeč
• lignin MeSH
• manganese peroxidase MeSH Prohlížeč
• oxidoreduktasy MeSH
• peroxidasy MeSH

The potential use of fungal pellets for decolorization of the textile dyeing wastewater was evaluated. The live pellets of the fungus Phanerochaete chrysosporium were found to remove more than 95% of the color of this wastewater within 1 d. The dye-removal capacity was a function of time and was proportional to the agitation rate; the optimum temperature was 30 degrees C. Both live and dead pellets were further examined in a repeated-batch mode for 5 d. The decolorization performance of live pellets remained high and stable for 5 d and they showed twice to thrice higher decolorization capacity than dead pellets.

• MeSH
• biodegradace MeSH
• chemické látky znečišťující vodu metabolismus   MeSH
• indoly metabolismus   MeSH
• Phanerochaete metabolismus   MeSH
• textilní průmysl metody   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• chemické látky znečišťující vodu MeSH
• indirubin MeSH Prohlížeč
• indoly MeSH

The effect of enhanced laccase (Lac) activity (obtained after copper addition to cultivation media) on decolorization of azo dye Orange G in two basidiospore-derived monokaryotic isolates of Pleurotus ostreatus was determined. The high Lac-producing isolate efficiently decolorized Orange G. The low-producing isolate showed only poor decolorization ability during cultivation in liquid medium and no decolorization on agar plates containing Orange G after a 25-d growth. A substantial enhancement of Lac activity caused by copper addition into cultivation media was detected in both isolates but, at the same time, the biomass production decreased and decolorization rate was reduced.

• MeSH
• aktivace enzymů účinky léků   MeSH
• azosloučeniny metabolismus   MeSH
• lakasa metabolismus   MeSH
• látky znečišťující životní prostředí metabolismus   MeSH
• měď farmakologie   MeSH
• peroxidasy metabolismus   MeSH
• Pleurotus klasifikace   enzymologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• azosloučeniny MeSH
• lakasa MeSH
• látky znečišťující životní prostředí MeSH
• manganese peroxidase MeSH Prohlížeč
• měď MeSH
• Orange G MeSH Prohlížeč
• peroxidasy MeSH

Molasses wastewater (vinasse; the by-product of distillation of fermented sugar) was decolorized and its chemical oxygen demand (COD) was reduced in static cultivation using the fungi Coriolus versicolor, Funalia trogii, Phanerochaete chrysosporium and Pleurotus pulmonarius ('Pleurotus sajorcaju'). The effect of cotton stalk on decolorizing and COD removing capability of four fungi was determined. In the entire concentration range tested (10-30%), wastewater was effectively decolorized by C. versicolor and F. trogii. Cotton stalk addition stimulated the decolorization activity of all fungi. The utilization of cotton stalk represents several advantages due to its function as an attachment place and as a source of nutrients; its use also reduces process costs.

• MeSH
• barva MeSH
• biodegradace * MeSH
• fermentace MeSH
• houby metabolismus   MeSH
• melasa * MeSH
• mikrobiologie vody MeSH
• odpad tekutý - odstraňování MeSH
• Pleurotus metabolismus   MeSH
• Polyporales metabolismus   MeSH
• průmyslový odpad * MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• průmyslový odpad * MeSH