Genome analysis of Treponema zioleckii proved that, in this bacterium, besides chromosomal DNA, a relatively small extrachromosomal DNA element is present. This element was shown to be a double-stranded circular plasmid DNA of approximately 7 kbp; it was designated as pKT. The plasmid was characterized by molecular and bioinformatic analysis. No pKT homologous DNA sequences were detected in other rumen Treponema strains. The overall G+C content of the pKT plasmid is approximately 56 %, which is higher than in other Treponema plasmids or genomes. The Rep module of the pKT plasmid consisting of the rep gene and the region of repeats was identified within a 1.6-kbp fragment. The putative rep gene encodes the replication protein belonging to the pfam04796 RepA_C family of proteins with the highest similarity (25 % within 249 amino acids) to the RepA protein from the green sulfur bacterium Prosthecochloris aestuarii.

• MeSH
• bachor mikrobiologie   MeSH
• bakteriální proteiny genetika   MeSH
• DNA bakterií analýza   izolace a purifikace   MeSH
• fylogeneze MeSH
• genom bakteriální * MeSH
• hybridizace nukleových kyselin MeSH
• molekulární sekvence - údaje MeSH
• ovce MeSH
• plazmidy genetika   MeSH
• replikace DNA MeSH
• sekvence aminokyselin MeSH
• sekvenční analýza DNA MeSH
• Treponema genetika   izolace a purifikace   MeSH
• zastoupení bazí MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• bakteriální proteiny MeSH
• DNA bakterií MeSH