Plant secondary metabolism evolved in the context of highly organized and differentiated cells and tissues, featuring massive chemical complexity operating under tight environmental, developmental and genetic control. Biotechnological demand for natural products has been continuously increasing because of their significant value and new applications, mainly as pharmaceuticals. Aseptic production systems of plant secondary metabolites have improved considerably, constituting an attractive tool for increased, stable and large-scale supply of valuable molecules. Surprisingly, to date, only a few examples including taxol, shikonin, berberine and artemisinin have emerged as success cases of commercial production using this strategy. The present review focuses on the main characteristics of plant specialized metabolism and their implications for current strategies used to produce secondary compounds in axenic cultivation systems. The search for consonance between plant secondary metabolism unique features and various in vitro culture systems, including cell, tissue, organ, and engineered cultures, as well as heterologous expression in microbial platforms, is discussed. Data to date strongly suggest that attaining full potential of these biotechnology production strategies requires being able to take advantage of plant specialized metabolism singularities for improved target molecule yields and for bypassing inherent difficulties in its rational manipulation.

• Klíčová slova
• Genetically engineered cultures, In vitro culture, Natural products, Secondary metabolites, Synthetic biology,
• MeSH
• artemisininy izolace a purifikace   metabolismus   MeSH
• axenická kultura MeSH
• berberin izolace a purifikace   metabolismus   MeSH
• biologické přípravky izolace a purifikace   metabolismus   MeSH
• biotechnologie metody   MeSH
• buněčné kultury MeSH
• fytonutrienty biosyntéza   izolace a purifikace   MeSH
• metabolické inženýrství metody   MeSH
• naftochinony izolace a purifikace   metabolismus   MeSH
• paclitaxel biosyntéza   izolace a purifikace   MeSH
• rostlinné buňky chemie   metabolismus   MeSH
• rostliny chemie   genetika   metabolismus   MeSH
• sekundární metabolismus MeSH
• techniky tkáňových kultur MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• artemisinin MeSH Prohlížeč
• artemisininy MeSH
• berberin MeSH
• biologické přípravky MeSH
• fytonutrienty MeSH
• naftochinony MeSH
• paclitaxel MeSH
• shikonin MeSH Prohlížeč