Goeckerman therapy (GT) of psoriasis vulgaris is based on the application of crude coal tar and ultraviolet radiation. We investigated DNA damage by the number of micronucleated binucleated cells (MNBC) in lymphocytes, serum homocysteine, vitamin B12, folic acid, and two polymorphisms (C677T and A1298C) in the MTHFR gene in 35 patients with exacerbated psoriasis vulgaris classified according to the psoriasis area and severity index (PASI) score and treated by GT. The median of PASI score decreased from nineteen to five, and MNBC increased from 10 to 18‰ after GT (p < 0.001 in both cases). Correlations of MNBC with homocysteine (Spearman's rho = 0.420, p = 0.012) and vitamin B12 (rho = -0.389, p = 0.021) before the therapy were observed. Hyperhomocysteinemia was an independent predictor of genotoxicity (OR 9.91; 95% CI, 2.09-55.67; p = 0.003). Homocysteine was higher in females than in males (13 vs. 12 µmol/L, p = 0.045). In contrast, vitamin B12 levels in the females were lower than in the males (160 vs. 192 pmol/L, p = 0.047). Vitamin B12 in the females were negatively influenced by smoking status (160 pmol/L in smokers vs. 192 pmol/L in non-smokers, p = 0.025). A significantly higher MNBC was found in CC homozygous patients (A1298C polymorphism) than in AC heterozygotes (32 vs. 16‰, p = 0.005) and AA homozygotes (32 vs. 18‰, p = 0.036). Our data showed that homocysteine participates in the pathogenesis of psoriasis. Its serum levels correlated with MNBC and allowed the prediction of DNA damage to appear within GT. Both micronutrients status and homocysteine metabolic pathway contribute to the genotoxicity of GT.

• Klíčová slova
• Goeckerman therapy, folic acid, genotoxicity, homocysteine, psoriasis, vitamin B12,
• MeSH
• dehet uhelný terapeutické užití   MeSH
• dospělí MeSH
• homocystein krev   MeSH
• jednonukleotidový polymorfismus * MeSH
• keratolytika terapeutické užití   MeSH
• kyselina listová krev   MeSH
• lidé středního věku MeSH
• lidé MeSH
• methylentetrahydrofolátreduktasa (NADPH2) genetika   MeSH
• mikrojaderné testy MeSH
• mikroživiny krev   MeSH
• poškození DNA účinky léků   účinky záření   MeSH
• psoriáza krev   genetika   patologie   terapie   MeSH
• terapie ultrafialovými paprsky metody   MeSH
• vitamin B 12 krev   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• dehet uhelný MeSH
• homocystein MeSH
• keratolytika MeSH
• kyselina listová MeSH
• methylentetrahydrofolátreduktasa (NADPH2) MeSH
• mikroživiny MeSH
• MTHFR protein, human MeSH Prohlížeč
• vitamin B 12 MeSH